Antimicrobial Resistance of Escherichia coli Isolated from Chickens with Colibacillosis in and Around Harare, Zimbabwe

Saidi, Bamusi; Mafirakureva, Prettimore; Mbanga, Joshua

doi:10.1637/10325-081512-case.1

Cited by 40 publications

(32 citation statements)

References 13 publications

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“…This finding coincides with the findings of Karczmarczyk, et al, (2011) where resistance to Tetracycline was the highest followed by Cephalothin (cephalosporin), the Amoxicillin. This finding is also partly supported by the findings of Saidi et al, (2012) where E. coli isolates showed moderate rates of resistance to tetracycline and chloramphenicol.…”

Section: Discussionsupporting

confidence: 73%

Study of Antibiotic Resistance on Escherichia Coli in Commercial Poultry of Nepal

Khanal¹,

Raut²,

Paneru³

2017

Nep. Vet. J

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The continuous use of antibiotics in compound feed at sub-therapeutic level has been an integral part of commercial poultry production in Nepal, which is one of the factors that promotes bacterial resistance. Hence, with the objective to determine antibiotic resistance in commercial poultry of Nepal, this study was designed taking Escherichia coli as a flagship bacterium. The commercial layers and broilers birds brought to veterinary teaching hospital of Agriculture and Forestry University by commercial poultry producers for disease diagnosis and treatment were considered as clinical examination of birds were carried out followed by post mortem examination (PME). Those layer/broiler birds which were not taking antibiotic orally or parenterally for last 2 weeks and diagnosed with collibacillosis on PME were included in sampling frame. Air sacculitis, fibrinous pericarditis, fibrinous perihepatitis, and coligranuloma were major criteria for presumptive diagnosis of colibacillosis on PME. The first 40 for both broiler and layer birds totaling 80 that fulfilled the criteria were selected as samples, each representing a commercial farm. All necessary information on daily management practices and previous treatments were obtained from farmer's record book or sheets or face to face interview. Avian pathogenic E. coli was isolated from aseptically collected liver samples and confirmedby biochemical tests. Antibiogram of the isolates were investigated by means of Kirby-Bauer disc diffusion method. E. coli was isolated from all liver samples taken for the culture. It was found that E. coli were resistant most substantially towards Cephalexin (81.2%) and Amoxycillin (81.2%) followed by Tetracycline (78.8%), Colistin sulphate (n=50, 62.5%). Chloramphenicol (61.2%), Ciprofloxacin (55.0%), Enrofloxacin (53.8%), Levofloxacin (28.8%), however, no resistance was found against amikacin. The proportion of E. coli isolates that were resistance against Colistin sulphate (p<0.05), Chloramphenicol (p< 0.05), Tetracycline (p<0.001), Ciprofloxacin (p<0.01), Enrofloxacin (p<0.05) and Gentamicin (p<0.01) were significantly higher in layers compared to that of broilers. In conclusion, avian pathogenic E. coli were resistant towards several antibiotic molecules commonly used in commercial poultry of Nepal, and the resistance was higher in layers compared to broilers.

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Section: Discussionsupporting

confidence: 73%

Study of Antibiotic Resistance on Escherichia Coli in Commercial Poultry of Nepal

Khanal¹,

Raut²,

Paneru³

2017

Nep. Vet. J

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“…These findings are more or less similar to findings of Salehi and Bonab (2006) from Iran who reported similar antimicrobial resistance pattern of avian pathogenic E. coli isolates except that of ampicillin. Regarding the resistance of ampicillin in this study, findings corroborate with the findings of Saidi et al (2013) from Zimbabwe. From the present study, lowest resistance of E. coli isolates was observed to amikacin (25.6%) followed by amoxacillin-sulbactam (31.4%) and gentamicin (32.5%).…”

Section: Discussionsupporting

confidence: 90%

Isolation, virulence gene profiling with molecular cloning of ibeA gene and antibiogram of Escherichia coli from respiratory tract infections of broiler chicken in Kashmir, India

Nyrah

Wani

Nazir

et al. 2017

JANS

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he present study has determined prevalence, serological diversity, virulence gene profileand in vitro antibiogram of Escherichia coli (E. coli) isolates from respiratory tract of broiler chicken in Kashmir valley along with molecular cloning of ibeA gene, an important zoonotic among invasion-associated genes responsible for neonatal meningitis in humans. A total of 224 broilerchickens with a history of respiratory tract infections, in a flock size of 2692 birds from organised and unorganised poultry farms of Kashmir valley, were screened for E. coli isolation. The prevalence of E. coli in birds with respiratory tract infection was found to be 6.38 per cent. Serogrouping revealed that the E. coli isolates were typeable into 10 serogroups with prevalence rate of 8.8% for serogroup O2, along with few isolates rough or untypeable. The screening of E. coli isolates for virulence gene profile revealed the presence of iss in (89.53%), iucDin (85.46 %), tsh in (51.74 %), cva/cvi in (33.14 %), irp2in (55.23 %), papCin (33.72 %), vat in (31.39 %), astAin (38.95 %), fimCin (93.60 %) and ibeAin (1.16 %) isolates. The antibiogram screening of E. coli isolates revealed majority of isolates to be resistant to tetracycline (94.71%), ampicillin (92.67%) and cephalaxin (79.67%).However, most of the isolates were sensitive to amikacin (74.41%) followed by amoxacillin-sulbactam (68.60%) and gentamicin (67.44%). The study thus, suggests that the E. coli isolates from respiratory tract infection of poultry harbour differential virulence genes along with the genes of zoonotic interest and variable antimicrobial resistance, thus, opening the areas for devising dynamic, emergent and effective treatment line.

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“…4). Saidi et al (2016) showed that there were high prevalence of sensitivity of avian pathogenic E. coli (APEC) to ciprofloxacin (100%) and gentamycin (97.1%). The isolates showed moderate rates of sensitivity to chloramphenicol and neomycin in Harare, Zimbabwe.…”

Section: Antibiotic Sensitivity Testmentioning

confidence: 99%

Effect of Age and Season on Enteritis and Antibiotic Sensitivity Test of E. coli Isolated from Infected Chickens in Odisha, India

Majhi¹,

Pamia²,

Panda³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Antimicrobial Resistance of Escherichia coli Isolated from Chickens with Colibacillosis in and Around Harare, Zimbabwe

Cited by 40 publications

References 13 publications

Study of Antibiotic Resistance on Escherichia Coli in Commercial Poultry of Nepal

Study of Antibiotic Resistance on Escherichia Coli in Commercial Poultry of Nepal

Isolation, virulence gene profiling with molecular cloning of ibeA gene and antibiogram of Escherichia coli from respiratory tract infections of broiler chicken in Kashmir, India

Effect of Age and Season on Enteritis and Antibiotic Sensitivity Test of E. coli Isolated from Infected Chickens in Odisha, India

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