Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant

Łuczkiewicz, Aneta; Jankowska, Katarzyna; Fudala-Książek, Sylwia; Olańczuk-Neyman, K.

doi:10.1016/j.watres.2010.08.007

Cited by 205 publications

(168 citation statements)

References 36 publications

Supporting

Mentioning

145

Contrasting

Unclassified

Order By: Relevance

“…E. coli isolates with MDR phenotype were detected in all samples, comprising from 6 % (in VR and DMOut) to 13 % (in WMOut) of isolates. The positive selection of bacteria with resistance patterns, previously described in wastewater processes (Łuczkiewicz et al 2010;Ferreira da Silva et al 2007;Novo and Manaia 2010), was also observed in this study for both of the WWTPs. Resistance rate to AMC, STX, CIP, and LVX noted for E. coli isolated from treated wastewater (WTW and DTW) was higher than that observed in corresponding raw wastewater (WRW and DRW), but the differences were not statistically significant (in Fisher's exact test).…”

Section: E Coli With Antibiotic and Multiple-antibiotic Resistancesupporting

confidence: 85%

“…However, other important aspects of wastewater discharge are currently under debate. It is suspected that clinically relevant bacteria and mobile genetic elements can survive the wastewater treatment plant processes (Reinthaler et al 2003;D'Costa et al 2006;Łuczkiewicz et al 2010) and be disseminated in the receiving waters (Iwane et al 2001;Li et al 2009;Czekalski et al 2012). Additionally, human-associated bacteria are regarded as important vectors of gene transmission (D'Costa et al 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

2015

Wastewater and Public Health

View full text Add to dashboard Cite

In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n=84) and 3.05 % (n=8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

show abstract

Section: E Coli With Antibiotic and Multiple-antibiotic Resistancesupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

2015

Wastewater and Public Health

View full text Add to dashboard Cite

show abstract

“…The multiple resistance frequency of E. coli was very high up to 90.6% in the present study, which is much higher than 24.2% for the Italian study. 32 It suggested that more serious contamination with ARB occurred in Plant A and Plant B. Tetracycline had the highest resistance frequency of 70.8%, which was much higher than that of Łuczkiewicz et al (23%), 24 followed by ampicillin (65.1%) ( Table 4). As a third generation cephalosporin, ceazidime was observed to have the lowest frequency of 9.0% in the present study.…”

Section: Discussionmentioning

confidence: 89%

Antibiotic resistance, plasmid-mediated quinolone resistance (PMQR) genes and ampC gene in two typical municipal wastewater treatment plants

Ying

et al. 2014

Environ. Sci.: Processes Impacts

View full text Add to dashboard Cite

Antibiotic resistant bacteria and plasmid-mediated quinolone resistance genes and ampC gene were investigated for Escherichia coli isolates from two typical municipal wastewater treatment plants in both dry and wet seasons by using the antibiotic susceptibility test and PCR assay, respectively. The results showed that 98.4% of the isolates (1056) were found resistant to antibiotic(s) tested and 90.6% showed multiple resistances to at least three antibiotics. Tetracycline was found to have the highest resistance frequency (70.8%), followed by ampicillin (65.1%), whereas ceftazidime had the lowest resistance frequency of 9.0%.Moreover, 39.2% of the E. coli isolates were carrying plasmids. intI1 had the highest detection rate in the plasmids (38.1%), followed by qnrS, ampC, qnrB, intI2 and aac(6 0 )-Ib-cr. The disinfection process (UV and chlorination) could significantly reduce the number of bacteria, but percentage of the resistant bacteria, resistance frequency for each antibiotic, MAR index and detection rate of the plasmid-mediated resistance genes were all found increasing in the effluents of biological units. The results of this study showed that a more frequent horizontal gene transfer occurred in the biological units. Wastewater treatment plants were an important medium for the recombination and dissemination of antibiotic resistance genes in the environment. Environmental impactThe wide application of antibiotics can lead to environmental contamination with antibiotic residues and development of bacterial resistance to antibiotics. This has become a global public concern. A variety of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) were widely detected in various environmental media. Effluents from wastewater treatment plants (WWTPs) are suspected to be one of the main anthropogenic sources for ARGs and ARB. Therefore, it is important to understand the removal and dissemination of ARGs and ARB in WWTPs. Plasmids are the major vectors of horizontal gene transfer (HGT). Quinolone resistance genes and ampC gene are two kinds of ARGs contained in the plasmids normally found in many Enterobactericeae isolated from clinical samples. Unfortunately, little is known about their occurrence and characteristics in WWTPs. This study aimed to investigate the antibiotic resistance proles in Escherichia coli isolated from two typical municipal wastewater treatment plants (activated sludge and oxidation ditch processes), to characterize plasmid-mediated quinolone resistance genes, ampC gene and integrons by the polymerase chain reaction (PCR), and to assess the effects of treatment processes on the removal of ARB and ARGs in the WWTPs. The results showed that the treatment processes especially disinfection processes (UV and chlorination) could signicantly reduce the number of bacteria, but increase bacterial resistance in the biological treatment units. The ndings reect the importance of WWTPs as a reservoir of ARB and ARGs, and as a medium for their recombination and dissemination in the en...

show abstract

“…The observed bacterial elimination (2 log) after passage through the WWTP is typical of activated sludge processes (Łuczkiewicz et al, 2010;Novo and Manaia, 2010). Although more than 90% of integrons were eliminated, the normalized copy number of class 1 and class 2 integrons was not reduced, and around 10 16 integrons were released each day into environmental water ( Figure 5).…”

Section: Hospital Effluent Impact On Integron Dissemination T Staldermentioning

confidence: 93%

Quantitative and qualitative impact of hospital effluent on dissemination of the integron pool

et al. 2013

View full text Add to dashboard Cite

There is increasing evidence that human activity, and especially the resulting effluent, has a major role in the dissemination of bacterial antibiotic-resistance determinants in the environment. Hospitals are the major antibiotic consumers and thus facilitate the spread of antibiotic resistance. Questions are increasingly being raised about the management of hospital effluents, but their involvement in antibiotic-resistance dissemination has never been assessed. Integrons are a paradigm of genetic transfer between the environmental resistome and both commensal and pathogenic bacteria. In order to assess the impact of hospital activities on antibiotic-resistance dissemination in the environment, we monitored integrons and their gene cassettes in hospital effluents, and their release in the environment. We found that bacterial communities present in a hospital effluent contained a high proportion of integrons. In terms of both their gene cassette diversity and gene cassette arrays, the urban effluent and municipal wastewater treatment plant (WWTP) influent were most similar, whereas the hospital effluent and recirculation sludge exhibited very specific patterns. We found that anthropogenic activities led to the release of abundant integrons and antibiotic-resistance gene cassettes, but we observed no specific impact of hospital activities on the receiving environment. Furthermore, although the WWTP did not reduce the normalized integron copy number, it reduced the diversity of gene cassette arrays contained in the raw wastewater, underlining the effect of the biological treatment on the anthropogenic integron pool arriving at the WWTP.

show abstract

Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant

Cited by 205 publications

References 36 publications

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

Antibiotic resistance, plasmid-mediated quinolone resistance (PMQR) genes and ampC gene in two typical municipal wastewater treatment plants

Quantitative and qualitative impact of hospital effluent on dissemination of the integron pool

Contact Info

Product

Resources

About