Antimicrobial Resistance of
            <i>Listeria monocytogenes</i>
            Strains Isolated from Humans in France

Morvan, Anne; Moubareck, Carole Ayoub; Leclercq, Alexandre; Hervé-Bazin, M.; Brémont, Sylvie; Lecuit, Marc; Courvalin, Patrice; Monnier, Alban Le

doi:10.1128/aac.01557-09

Cited by 217 publications

(195 citation statements)

References 39 publications

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“…has been studied in various food, environmental and clinical settings (Bertrand et al, 2005;Morvan et al, 2010;Granier et al, 2011;Jamali et al, 2015). Listeria monocytogenes has generally been shown to be more susceptible to antimicrobial agents then other species in the genus.…”

Section: Resultsmentioning

confidence: 99%

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Nielsen

Björkman

Kiil

et al. 2017

EFSA Supporting Publications

111

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This report presents the results of the project "Closing gaps for performing a risk assessment on Listeria monocytogenes in ready-to-eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis". The main objective was to compare L. monocytogenes isolates collected in the EU from ready-to-eat (RTE) foods, compartments along the food chain and from human cases by the use of WGS. A total of 1,143 L. monocytogenes isolates were selected for the study, including 333 human clinical isolates and 810 isolates from the food chain. The isolates were whole genome sequenced. The phylogeny showed a clear delineation between L. monocytogenes lineages and between clonal complexes within lineages. A range of typing methods were applied to the sequence data, providing the framework to answer questions on genetic diversity and epidemiological relationships. Retrospective analysis of nine outbreaks showed that WGS is a powerful tool in national and international outbreak investigations as WGS can accurately rule isolates in or out of outbreaks. Source attribution models showed bovine reservoir to be the main source of human disease although other sources also contributed and generally confidence intervals were high. Numerous consistent genetic linkages between a priori unlinked strains were identified, some of which involved isolates from multiple countries. The presence of putative markers conferring the potential to survive/multiply in the food chain and/or cause disease in humans was explored by detecting the presence of putative virulence genes, AMR genes and factors conferring the ability to persist in the food processing chain. This study has demonstrated one of the major benefits of WGS, which is the ability to address a wide range of questions including those on virulence, antimicrobial resistance, source attribution, surveillance and outbreak detection and investigation, in a single experiment.

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Section: Resultsmentioning

confidence: 99%

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Nielsen

Björkman

Kiil

et al. 2017

EFSA Supporting Publications

111

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“…On the other hand B. cereus and L. monocytogens are also Gram-positive bacteria like S. aureus but they are more resistant to carvacrol microemulsion Table 2 . That could be due to the spore forming nature of B. cereus or the presence of specific antimicrobial drug resistant genes in this microorganism 57 and in L. monocytogens 58,59 which are not present in S. aureus. Table 3 showed that formulation of carvacrol in microemulsion using the cationic surfactant CPC had significantly higher antibacterial activity than that formulated with the non-ionic T20.…”

Section: Antimicrobial Activity Of Carvacrol Microemulsionmentioning

confidence: 99%

Factors Affecting the Phase Behavior and Antimicrobial Activity of Carvacrol Microemulsions

Shaaban¹,

Edris²

2015

J. Oleo Sci.

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“…It appears that virtually the same profile has been identified over the years and in different countries. In relation to tetracycline, this research highlighted the extreme sensitivity of the 68 strains to this drug, contrasting with the emergence of clinical strains resistant to tetracycline, related to the gene tetM 5,22,23 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil

Reis

Barbosa

Rusak

et al. 2011

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented by chloramphenicol, erythromycin, tetracycline and rifampicin. The aim of this study was to analyze the antimicrobial susceptibility profile of strains isolated from human sources in the last four decades. METHODS: Sixty-eight strains were selected from the culture collection of the Laboratory of Bacterial Zoonoses/LABZOO/FIOCRUZ isolated in different regions of Brazil from 1970 to 2008 and primarily isolated from cerebrospinal fluid and blood culture. Susceptibility tests to antimicrobials drugs were evaluated using the criteria established by Soussy using the Kirby-Bauer method and E-Test strips were used to determine the minimum inhibitory concentration (MIC). RESULTS: Among the strains tested, serovar L4b (60.3%) was the most prevalent, followed by serovar 1/2a (20.6%), 1/2b (13.2%) and the more uncommon serovars 1/2c, 3b and 4ab (5.9%). All strains were susceptible to ampicillin, cephalothin, erythromycin, gentamicin, teicoplanin and vancomycin. Only one strain (1.5%) showed resistance to rifampin, and two (3%) were resistant to trimethoprim-sulfamethoxazole. MICs with values up to 2μg/ml reinforce the need for microbiological surveillance. CONCLUSIONS: The study demonstrated low prevalence of strains resistant to the antimicrobial drugs indicated in the treatment of human listeriosis. Monitoring antimicrobial resistance profile is still very important to determine adequate treatment, especially in immunocompromised patients.

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Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans in France

Cited by 217 publications

References 39 publications

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Factors Affecting the Phase Behavior and Antimicrobial Activity of Carvacrol Microemulsions

Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil

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