Antimicrobial resistance ofPasteurella multocidastrains isolated from pigs between 2010 and 2016

Abstract

Pasteurella multocida is one of the significant causes of respiratory infection outbreaks in the Korean pig industry. Although antimicrobial treatment is an effective strategy for controlling respiratory diseases, limited information is available regarding the antimicrobial susceptibility of the pathogens infecting Korean pigs. Therefore, in this study, we evaluated the antimicrobial resistance of P multocida against widely used antimicrobials in order to enable the selection of appropriate drugs and to evaluate any trends in resistance. A total of 454 isolates of P multocida were collected from all provinces in Korea between 2010 and 2016. Antimicrobial susceptibility of all isolates was determined using a broth microdilution method. The most frequently observed resistance was to sulphadimethoxine (76.0 per cent), followed by oxytetracycline (66.5 per cent), chlortetracycline (36.8 per cent) and florfenicol (18.5 per cent). Although no consistent increase or decrease in resistance was observed for most antimicrobials, resistance to fluoroquinolones tended to increase over the study period. A variety of resistance patterns were observed, most frequently for tetracyclines and sulphonamides. These findings could provide information enabling the selection of optimal antimicrobials for efficient treatment of pneumoniae pasteurellosis in pig farms, which would impede the emergence of antimicrobial resistance.

Antimicrobial resistance of Pasteurella multocida type B isolates associated with acute septicemia in pigs and cattle in Spain

“…Though previous authors found a high level of susceptibility (< 3% of isolates were resistant) to this drug [18,24,25,32], in our study 17.3% of P. multocida type B isolates from swine were resistant to it. In agreement with our results, a study carried out in Korea indicated that 18.5% of type A or D P. multocida isolates from pigs were resistant to florfenicol [27]. This drug, a phenicol used widely in veterinary medicine to treat pneumonia caused by P. multocida, belongs to the same antimicrobial group as chloramphenicol.…”

“…Petrocchi-Rilo et al [25] found 96.9% resistance (31/32 isolates) to clindamycin, while El Garch et al [28] described in Europe MIC values to lincomycin between 4 and 64 μg/mL in porcine isolates (the mode value was 32 μg/mL). According to the break point specified by the Clinical and Laboratory Standards Institute (CLSI) for lincomycin, all Petrocchi-R et al [25] Tang et al [18] Furian et al [26] El Garch et al [28] Timsit et al [29] Oh et al [27] This study isolates from El Garch et al [28] would have been classified as resistant. The low activity of lincosamides against Pasteurellaceae is well known [31] and the selection of this antimicrobial in the study reflects in the number of MDR patterns identified.…”

“…According to El Garch et al, the resistance rate of P. multocida isolates to tetracycline in Europe and Australia (between 11.5 and 32.2%) were the highest from among several antimicrobials tested [28]. Higher resistance values have been obtained for type A or D P. multocida from swine for tetracycline in China (58% [18]; and oxytetracycline in Korea (66.5% [27];, which may be related to the heavy use of antimicrobials in these countries compared to the European Union, especially in the pig industry [27]. Furian et al [26] also found high resistance to tetracycline in Brazil with 40% of the type A or D P. multocida isolates they had derived from pigs being resistant to this drug.…”

“…To the best of our knowledge, this study represents the first research performed in Europe about the antimicrobial susceptibility of capsular type B P. multocida derived from clinical samples from pigs and cattle affected with acute septicemia. However, the antimicrobial susceptibility of capsular types A and D from porcine isolates have been previously discussed for Spain [24,25], China [18], Brazil [26], and Korea [27]. In addition, other authors have studied antimicrobial resistance rates in P. multocida isolates from cattle in Europe [28] and the United States [29].…”

“…The percentage of P. multocida type B isolates resistant to penicillin may be lower because acute septicemia has appeared in Spain relatively recently. In contrast, ß-lactams remain effective in Korea where, according to one report, only 5.5% of P. multocida isolates from pigs were resistant to penicillin and 4.8% were resistant to ampicillin [27].…”