Antimicrobial Resistance of Vibrio Strains from Brackish Water on the Coast of Gyeongsangnamdo

Eun-Gyoung, OH; Son, Kwang-Tae; Ha, Kwang-Soo; Yoo, Hyun-Duk; Yu, Hong-Sik; Shin, Soon-Bum; Hee-Jung, Lee; Kim, Jihoe

doi:10.5657/kfas.2009.42.4.335

Cited by 10 publications

(10 citation statements)

References 31 publications

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“…Vibrio isolates from marine sources were known to possess a higher resistance to ampicillin (Ferrini, Mannoni, Suffredini, Cozzi, & Croci, ; Han, Walker, Janes, Prinyawiwatkul, & Ge, ). Particularly, 85.9% to 100% of the V. parahaemolyticus isolates ever collected from Korean coastal areas, estuaries and 96.9% of isolates from farmed fish and shellfish were found to be resistant to ampicillin (Kim et al, ; Lee, Lim, & Kim, ; Oh et al, ). Though, we could detect 100% resistance to ampicillin similarly as in a report from Korea about seafood‐borne V. parahaemolyticus (Jun et al, ).…”

Section: Resultsmentioning

confidence: 99%

Occurrence, virulence factors, and antimicrobial susceptibility patterns of Vibrio spp. isolated from live oyster (Crassostrea gigas) in Korea

Dahanayake

Silva

Hossain

et al. 2018

Journal of Food Safety

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This study determined the incidence, virulence factors, and antimicrobial susceptibility patterns of Vibrio spp. in live oysters (Crassostrea gigas) marketed in Korea. A total of 41 Vibrio spp. isolates including 23 Vibrio alginolyticus, 11 Vibrio fluvialis and 7 Vibrio antiquarius were isolated and identified. All Vibrio spp. isolates showed DNase, amylase, lipase, phospholipase, and gelatinase activities. For slime production, caseinase and β‐hemolysis isolates were positive in 68%, 78%, and 56%, respectively. PCR‐based assays showed that V. alginolyticus expressed collagenase, tlh, toxR, ctxAB, and VPI virulence genes in 100%, 100%, 78%, 39%, and 48% of the isolates, respectively. All V. fluvialis strains harbored hupO gene while 64% and 55% isolates were positive for toxR and vfh. Besides, V. antiquarius strains were positive for toxR, tlh, ctxAB, and VPI virulence genes in 57%, 86%, 43%, and 43% of the isolates, respectively. Multiple Antibiotic Resistance (MAR) index calculated with disk diffusion test results revealed that 54% of the isolates were having MAR indices >0.2. Our results reveal that consumption of raw oysters may cause potential risk for public health owing to the Vibrio spp. comprising putative virulence properties and antimicrobial resistance. Practical applications The genus Vibrio are most commonly associated with seafood‐borne diseases. Oysters are one of the most preferred seafood in Korea as well as throughout the world. However, it may harbor pathogenic Vibrio spp., can cause foodborne illnesses if consumed as raw or undercooked product. In this study, the prevalence and characteristics of Vibrio spp. isolated from oysters in Korean markets was investigated. Among a total of 41 isolates presumptively identified by biochemical tests as vibrios, the incidence of three Vibrio species was confirmed by gyrB sequencing. Most of the isolates showed positive results for pathogenicity tests and virulence gene tests indicating the putative virulence of Vibrio spp. Susceptibility for 14 antimicrobials were tested using disk diffusion method. All the isolates were resistant to ampicillin while resistance to some other antimicrobials were also observed. According to these results, it is worthwhile to raise consumer awareness that consumption of oysters from markets in Korea may pose a risk of Vibrio infection.

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Section: Resultsmentioning

confidence: 99%

Occurrence, virulence factors, and antimicrobial susceptibility patterns of Vibrio spp. isolated from live oyster (Crassostrea gigas) in Korea

Dahanayake

Silva

Hossain

et al. 2018

Journal of Food Safety

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“…The expected size of the amplified DNA was 251 bp for the tdh gene and 250 bp for the trh gene, respectively. Oh et al (2009) conducted a study to investigate the presence of the tdh and trh toxic genes in V. parahaemolyticus isolated from brackish water samples collected off the south coast of Korea, such as Geoje city, Tongyeong city, and Goseong Gun (Table 2). According to their results, 23 of 177 isolates were positive for the tdh gene, whereas all strains tested negative for the trh gene.…”

Section: Virulence Genes Of V Parahaemolyticus Isolates In Koreamentioning

confidence: 99%

“…About 15 of the isolates were resistant to three or more antibiotic agents, including tetracycline or ciprofloxacin, which are usually prescribed for V. parahaemolyticus infections. Oh et al (2009) investigated the antibiotic susceptibility of V. parahaemolyticus by analyzing the toxigenic and non-toxigenic strains isolated from brackish water samples on the south coast of Korea, such as in Geoje city, Tongyeong city, and Goseong Gun, to 15 antibiotics. A total of 177 V. parahaemolyticus strains were analyzed including 13% of the isolates with the tdh gene.…”

Section: Antibiotic Resistance Profiles Of V Parahaemolyticus Isolatmentioning

confidence: 99%

Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review

et al. 2018

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Background: Vibrio parahaemolyticus is one of the most common causes of seafood-borne illnesses in Korea, either directly or indirectly, by consuming infected seafood. Many studies have demonstrated the antibiotic susceptibility profile of V. parahaemolyticus. This strain has developed multiple antibiotic resistance, which has raised serious public health and economic concerns. This article reviews the food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of V. parahaemolyticus in Korea during 2003-2016. Main body: V. parahaemolyticus infections appeared to be seasonally dependent, because 69.7% of patient infections occurred in both August and September during 2003-2016. In addition, the occurrence of V. parahaemolyticus in marine environments varies seasonally but is particularly high in July, August, and September. V. parahaemolyticus isolated from aquaculture sources on the Korean coast varied in association with virulence genes, some did not possess either the tdh (thermostable direct hemolysin) or trh (tdh-related hemolysin) genes, and a few were positive for only the trh gene or both genes. The high percentage of ampicillin resistance against V. parahaemolyticus in the aquatic environment suggests that ampicillin cannot be used to effectively treat infections caused by this organism. Short conclusion: This study shows that the observed high percentage of multiple antibiotic resistance to V. parahaemolyticus is due to conventionally used antibiotics. Therefore, monitoring the antimicrobial resistance patterns at a national level and other solutions are needed to control aquaculture infections, ensure seafood safety, and avoid threats to public health caused by massive misuse of antibiotics.

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“…, (Neela et al, 2007;Oh et al, 2009 AM, Ampicillin; C, Chloramphenicol; CEP, Cefepime; CIP, Ciprofloxacin; GM, Gentamicin; CTT, Cefotetan; CTX, Cefotaxime; E, Erythromycin; K, Kanamycin; CF, Cephalothin; NA, Nalidixic acid; RA, Rifampin; S, Streptomycin; SXT, SulphamethoxazoleTrimethoprim; TE, Tetracyclin.…”

Section: 장염비브리오균 검출 현황 및 독소 유전자 보유율mentioning

confidence: 99%

Virulence Factors and Antimicrobial Susceptibility of Vibrio parahaemolyticus Isolated from the Oyster Crassostrea gigas

Kim¹,

An²,

Park³

et al. 2016

Korean Journal of Fisheries and Aquatic Sciences

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This study investigated the prevalence of Vibrio parahaemolyticus in the oyster Crassostrea gigas, which is commonly consumed raw. The presence of virulence factors and the antimicrobial susceptibility of isolates were also investigated. The overall prevalence rate of V. parahaemolyticus in oysters was 37.5% (36/96) and the range of concentrations was 30-11,000 MPN/100 g. PCR-based assays indicated that 9.6% (11/115) of the isolates were positive for the thermostable direct hemolysin-related hemolysin gene (trh), while none of the isolates were positive for the thermostable direct hemolysin gene (tdh). The Multiple Antibiotics Resistance (MAR) index was measured for 16 common antimicrobial agents and 46.1% (53/115) of the isolates had a MAR index > 0.2. The MAR index ranged from 0.07 to 0.73. The highest MAR index was observed with strain s150608, isolated in June 2015, which exhibited resistance to 11 antimicrobial agents. Our results demonstrate that oysters are high-risk sources of V. parahaemolyticus, although no antimicrobial agent was being used to promote growth or to treat bacterial infections in the sampled oyster-growing areas.

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Antimicrobial Resistance of Vibrio Strains from Brackish Water on the Coast of Gyeongsangnamdo

Cited by 10 publications

References 31 publications

Occurrence, virulence factors, and antimicrobial susceptibility patterns of Vibrio spp. isolated from live oyster (Crassostrea gigas) in Korea

Occurrence, virulence factors, and antimicrobial susceptibility patterns of Vibrio spp. isolated from live oyster (Crassostrea gigas) in Korea

Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review

Virulence Factors and Antimicrobial Susceptibility of Vibrio parahaemolyticus Isolated from the Oyster Crassostrea gigas

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