Antimicrobial Resistance Pattern in E. coli Isolated from Placental Tissues of Pregnant Women in Low-Socioeconomic Setting of Pakistan

Saraf, Viqar Sayeed; Bhatti, Theophilus; Javed, Sundus; Bokhari, Habib

doi:10.1007/s00284-022-02769-w

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…aureus, and P. aeruginosa, three of the most common bacterial strains accountable for tissue infection and healing failure. − As expected, coaxial membranes showed a lower antibacterial activity with respect to non-coaxial structures, being effective only against S. aureus, but not toward E.…”

Section: Discussionmentioning

confidence: 52%

Tuning the Drug Release from Antibacterial Polycaprolactone/Rifampicin-Based Core–Shell Electrospun Membranes: A Proof of Concept

Gruppuso

Guagnini

Musciacchio

et al. 2022

ACS Appl. Mater. Interfaces

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The employment of coaxial fibers for guided tissue regeneration can be extremely advantageous since they allow the functionalization with bioactive compounds to be preserved and released with a long-term efficacy. Antibacterial coaxial membranes based on poly-ε-caprolactone (PCL) and rifampicin (Rif) were synthesized here, by analyzing the effects of loading the drug within the core or on the shell layer with respect to non-coaxial matrices. The membranes were, therefore, characterized for their surface properties in addition to analyzing drug release, antibacterial efficacy, and biocompatibility. The results showed that the lower drug surface density in coaxial fibers hinders the interaction with serum proteins, resulting in a hydrophobic behavior compared to non-coaxial mats. The air-plasma treatment increased their hydrophilicity, although it induced rifampicin degradation. Moreover, the substantially lower release of coaxial fibers influenced the antibacterial efficacy, tested against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Indeed, the coaxial matrices were inhibitory and bactericidal only against S. aureus, while the higher release from non-coaxial mats rendered them active even against E. coli. The biocompatibility of the released rifampicin was assessed too on murine fibroblasts, revealing no cytotoxic effects. Hence, the presented coaxial system should be further optimized to tune the drug release according to the antibacterial effectiveness.

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Section: Discussionmentioning

confidence: 52%

Tuning the Drug Release from Antibacterial Polycaprolactone/Rifampicin-Based Core–Shell Electrospun Membranes: A Proof of Concept

Gruppuso

Guagnini

Musciacchio

et al. 2022

ACS Appl. Mater. Interfaces

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“…Rizvi et al, reported CRE prevalence of 4.5% in COVID-19 patients [ 120 ]. Saraf et al, reported imipenem resistant E. coli from mothers with preterm deliveries from low socioeconomic settings [ 121 ]. Afridi et al, reported an overall CRE frequency of 6.5% (136/2100) in Karachi population with all isolates sensitive to colistin, tigecycline, and fosfomycin [ 122 ].…”

Section: Resultsmentioning

confidence: 99%

A systematic review and meta-analysis of carbapenem resistance and its possible treatment options with focus on clinical Enterobacteriaceae: Thirty years of development in Pakistan

Umair,

Walsh,

Mohsin

2024

Heliyon

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“…The current study was conducted in continuation of our previously published work on E. coli induced UTIs as a risk factor for preterm births in Pakistan [ 55 , 56 ]. Another rationale for selection of the study population and UPEC as particular variant of Extraintestinal pathogenic Escherichia coli (ExPEC) was its significant relevance with a highly drug resistant and virulent clonal group A of E. coli [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections

Khan,

Saraf,

Siddiqui

et al. 2024

BMC Microbiol

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Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli. Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate the presence of clinically important strains of E. coli in Pakistani women having uncomplicated cystitis and pyelonephritis. Women belonging to low-income groups were exclusively included in the study. Seventy-four isolates from urine samples were processed, phylotyped, and screened for the presence of two Single Nucleotide Polymorphisms (SNPs) particularly associated with a clinically important clonal group A of E. coli (CgA) followed by antibiotic susceptibility testing and genome sequence analysis. Results Phylogroup B2 was most prevalent in patients and 44% of isolates were positive for the presence of CgA specific SNPs in Fumarate hydratase and DNA gyrase subunit B genes. Antibiotic susceptibility testing showed widespread resistance to trimethoprim-sulfamethoxazole and extended-spectrum beta-lactamase production. The infection analysis revealed the phylogroup B2 to be more pathogenic as compared to the other groups. The genome sequence of E. coli strain U17 revealed genes encoding virulence, multidrug resistance, and host colonization mechanisms. Conclusions Our research findings not only validate the significant occurrence of multidrug-resistant clonal group A E. coli (CgA) in premenopausal Pakistani women suffering from cystitis and pyelonephritis but also reveal the presence of genes associated withvirulence, and drug efflux pumps. The detection of highly pathogenic, antimicrobial-resistant phylogroup B2 and CgA E. coli strains is likely to help in understanding the epidemiology of the pathogen and may ultimately help to reduce the impact of these strains on human health. Furthermore, the findings of this study will particularly help to reduce the prevalence of uncomplicated UTIs and the cost associated with their treatment in women belonging to low-income groups.

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Antimicrobial Resistance Pattern in E. coli Isolated from Placental Tissues of Pregnant Women in Low-Socioeconomic Setting of Pakistan

Cited by 4 publications

References 31 publications

Tuning the Drug Release from Antibacterial Polycaprolactone/Rifampicin-Based Core–Shell Electrospun Membranes: A Proof of Concept

Tuning the Drug Release from Antibacterial Polycaprolactone/Rifampicin-Based Core–Shell Electrospun Membranes: A Proof of Concept

A systematic review and meta-analysis of carbapenem resistance and its possible treatment options with focus on clinical Enterobacteriaceae: Thirty years of development in Pakistan

Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections

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