Antimicrobial resistance, penicillin-binding protein sequences, and pilus islet carriage in relation to clonal evolution of Streptococcus pneumoniae serotype 19A in Russia, 2002–2013

Abstract

SUMMARYClonal changes of serotype 19A pneumococci have been appreciated in conjunction with growing prevalence of this serotype after implementation of the seven-valent pneumococcal conjugate vaccine (PCV7). In the present study, we characterized serotype 19A pneumococci collected in Russia within a decade preceding the implementation of PCV vaccination and described their clonal evolution. We retrospectively analyzed non-invasive serotype 19A isolates collected in 2002-2013. All isolates were subjected to multilocus sequence typing, antimicrobial susceptibility testing, determination of macrolide resistance genotype, molecular detection of pilus islet (PI) carriage, sequencing of penicillin-binding protein (PBP) genes. A total of 49 serotype 19A isolates represented 25 sequence types, of which 14 were newly described. The majority of isolates were distributed among clonal complex (CC) 663 (28%), CC230 (25%), CC156, and CC320 (14% each). CC663 and CC156 dominated in 2003, but were replaced by CC230 and CC320 later on; CC320 was only evident starting 2010. All isolates of CC663 and CC156 carried PI1; CC320 possessed both PI1 and PI2. The overall rate of altered amino acids in penicillin-nonsusceptible isolates was 13·9%, 7·2%, and 8·7% for PBP1a, PBP2b, and PBP2x, respectively. Our findings demonstrate that the clonal structure of serotype 19A pneumococci may evolve without PCV pressure.

Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines

“…A high incidence of IPD due to serotype 19A has been associated with a limited number of clonal complexes (CC199, CC320, and CC276). Contrary to that, a study from Russia carried out by Mayanskiy et al [50] demonstrated that local serotype distribution In our study, two isolates (1.8%) belonged to serotype 15A, which is not included in PCV-13. This serotype was previously associated with MDR and was isolated from most IPD cases in the post-PCV era in several post-industrial countries [51][52][53].…”

The prevalence of pilus islets in Streptococcus pneumoniae isolates from healthy children in Indonesia

“…In Russia, it was reported that among 49 S . pneumoniae isolates of serotype 19A isolated from healthy children, 59 % ( n =29) carried PI-1, whereas 14 % ( n =7) carried PI-2 [11]. In Japan, among 151 isolates of S.…”

“…In Russia, it was reported that among 49 S . pneumoniae isolates of serotype 19A isolated from healthy children, 59 % ( n =29) carried PI-1, whereas 14 % ( n =7) carried PI-2 [ 11 ]. In Japan, among 151 isolates of S. pneumoniae isolated from patients suffering on pneumococcal disease, 34 isolates that carried PIs belonged to serotypes 19F, 23F, 19A, 6E, 15B and 35B [ 12 ].…”

Antibiotic Resistance and Molecular Biological Characteristics of Non-13-Valent-Pneumococcal Conjugate Vaccine Serogroup 15 Streptococcus pneumoniae Isolated From Children in China

“…We wondered whether the prevalence of CC3397 in serogroup 15 S. pneumoniae results from antibiotic pressure. Many studies have reported clonal shift phenomena in other serotypes, as CC271 replaced CC983 among serotype 19F strains ( Li et al, 2013 ), ST81 replaced ST342 amongst serotype 23F pneumococcal isolates ( Ma et al, 2013 ), CC320 replaced CC230 in 19A strains ( Beall et al, 2011 ; Golden et al, 2015 ; Mayanskiy et al, 2017 ), and CC876 replaced CC875 in serotype 14 strains ( He et al, 2015 ). These examples of clonal shift phenomena in the same serotype may be caused by antibiotic pressure, as they followed the principle that CCs/STs expressing high antibiotic resistance will replace CCs/STs with lower antibiotic resistance.…”