Antimicrobial resistance profile in clinical isolates of Pseudomonas aeruginosa producing Extended-spectrum beta-lactamase (ESBL) at a teaching tertiary care hospital, Tamilnadu

Abstract

The spread of extended-spectrum betalactamase (ESBL)-producing clinical isolates of P. aeruginosa has dramatically increased worldwide and frequently reported in India. The present study was undertaken to identify the prevalence of ESBL producing Pseudomonas aeruginosa and its antimicrobial resistance profile. This prospective study was conducted at Clinical microbiology Laboratory of Sri Muthukumaran Medical college hospital and research Institute for a period of 9 months.. P. aeruginosa isolates from all samples were identified by standard protocols. Antibiotic sensitivity testing for all isolates was done using Kirby-Bauer disc diffusion method. Disc potentiation test was performed to detect ESBL production. ESBL producing pseudomonas aeruginosa(16/69) in this study was 23.18%. Prevalence rate of ESBL isolates among the MDR strains was 41.17%.We observed ESBL isolates with high resistance rate to tested antipseudomonal antibiotics than Non ESBL. All ESBL isolates were sensitive to Polymyxin and Colistin. This study gives an alarming sign towards high prevalence of cephalosporins due to ESBL emphasizes the need for early detection to prevent therapeutic failure. In addition ,our study results would be useful to formulate antibiotic policy and its compliances for effective infection control practices.