Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China

De-sheng, Meng; Yehui, Wu; Meng, Qingling; Qiao, Jun; Zhang, Xingxing; Ma, Shuai; Kuojun, Cai; Zhang, Jinsheng; Zibing, Cheng; Zhang, Zaichao; Xue-peng, Cai

doi:10.1016/j.jgar.2018.08.024

Cited by 36 publications

(37 citation statements)

References 32 publications

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“…Most of isolates, particularly obtained from pigs and chicken, in this study, were high resistance and multi-drug resistant. The high prevalence of resistance to antimicrobials in our study was similar to that reported in previous studies [1,29,33,35]. Antimicrobial agents are often used for the prevention and treatment of infectious diseases and growing promotion of food producing animals in intensive production.…”

Section: Discussionsupporting

confidence: 91%

“…In the present study, 4 MRSA were isolated from 3 pig samples and 1 chicken sample, but not other animals. The prevalence of MRSA in our studywas lower than most researches, both in China [1,29,33] and foreign countries [14,34]. However, the study developed in Chongqing did not detect mecA gene in all 32 identified goat-originated S. aureus [31].…”

Section: Discussioncontrasting

confidence: 84%

“…In this study, we investigated the prevalence of S. aureus in different food producing animals, including pigs, cattle, goats, rabbits and chicken, in Chongqing, China. To our understanding, this is the first comprehensive survey on livestock-associated S. aureus in Chongqing, although numerous epidemiological studies of this organism have been carried out in other regions of China [1,29,30], and a report about S. aureus isolated from goats in Chongqing [31] has been published. The average prevalence of S. aureus in our study was similar with those obtained from Jiangmen, China [30], but differed from other areas.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, a study carried out in Henan showed that 23.7% of animal samples collected from cows, swine, chicken and ducks, were positive for S. aureu [1]. Dan M et al showed that a high isolation rate of S. aureus (46.0%) was obtained from cattle farms in Xinjiang Province, China [29]. A longitudinal study in Ireland indicated the average prevalence of S. aureus colonization in pigs varied between 26% and 73% [32].…”

Section: Discussionmentioning

confidence: 99%

“…Although there have been a great deal of reports on the epidemiological studies of S. aureus originated from food producing animals in China in recent years [1,29,30], little is known about the prevalence and occurrence of this organism in Chongqing, China. In this study, we investigated the prevalence of S. aureus isolated from food animals in Chongqing.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Wang

Yao

Jia

et al. 2019

Preprint

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Background Livestock-associated Staphylococcus aureus is one of the most important etiological agents in both human and animals. It has been reported with high antimicrobial resistance and multiple staphylococcal superantigen genes in many countries and several provinces of China. However, large-scale investigation of this organism has not been documented in Chongqing, China. The aim of this study is to demonstrate the prevalence, antimicrobial susceptibility and some molecular characteristics of S. aureus acquired from animals in Chongqing. Results A total of 89 S. aureus isolates were cultured from 1371 samples picked up from March 2014 to December 2017. The isolates were originated from pigs (25), cattle (6), goats (10), rabbits (16) and chicken (32). Four MRSA strains were identified from 3 pig samples and 1 chicken sample. The isolates showed high resistance to penicillin (93.3%) and ampicillin (92.1%), but were more susceptible to amikacin and ofloxacin, since the resistance rates of these two drugs were less than 10%. Meanwhile, 74.2% isolates exhibited varying degree of MDR. Almost all strains, except for 3 chicken-originated isolates, were positive for blaTEM-1a, but did not harbor other ESBL genes. Nineteen staphylococcal SE/SEl/TSST-1 genes, except seq, were detected in isolates. The predominant genes were sei (58.4%), tst-1 (56.2%) and seg (51.7%). Conclusions The high antimicrobial resistance and prevalence of blaTEM-1a seriously reminded that it was urgent to standardize and cut down the usage of antimicrobials. The universal existence of staphylococcal toxin genes in isolated strains implied a potential threat of public health from animals to human through the food chain.

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Wang

Yao

Jia

et al. 2019

Preprint

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Prevalence and characterization of Staphylococcus aureus from different processing points in beef processing plants in Shandong, China

Zhang,

Liu,

Yang

et al. 2024

Food Safety and Health

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Staphylococcus aureus (S. aureus) is a common zoonotic foodborne pathogen that poses a serious threat to the microbial control of meat processing, especially the multidrug resistant (MDR) strain. This study aimed to investigate the prevalence, antimicrobial susceptibility, virulence genes, biofilm formation ability (BFA), and molecular types of S. aureus isolated from two beef‐processing plants in China. The results revealed that the prevalence of S. aureus was 17.1% (60/350), including one methicillin‐resistant strain. The chilled carcass showed the highest detection rate among the seven sampling points. The isolates exhibited high resistance to penicillin (88.3%) and lincomycin (80.0%), and 75.0% of the isolates showed multidrug resistance (MDR). All isolates carried hla and clfa and critical toxin genes (seb, pvl, and tst) were also detected. Additionally, 96.7% of isolates exhibited strong and moderate BFA. The isolates were typed by Multilocus Sequence Typing (MLST) and Staphylococcus Protein A (spa) typing methods, resulting in nine different types for each method. The predominant type was ST7‐t091, accounting for 68.3% of the isolates. Our research suggests that there may be a critical control point in the chilling room, and the high MDR rate and strong BFA increase the difficulty of control and the risk of transmission.

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Prevalence, antimicrobial resistance, and staphylococcal toxin genes of bla_TEM‐1a‐producing Staphylococcus aureus isolated from animals in Chongqing, China

Dong

Wang

Zhang

et al. 2022

Veterinary Medicine & Sci

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Background Staphylococcus aureus infection of livestock animals and humans is a major public health issue. There are reports of antimicrobial resistance and multiple staphylococcal superantigen genes in many countries and several provinces of China, but the status in Chongqing, China is uncertain. Objectives The aim of this study was to determine the prevalence, antimicrobial susceptibility, and other molecular characteristics of S. aureus isolates from livestock animals in Chongqing. Methods Staphylococcus aureus was isolated and identified by selective enrichment and amplification of the nuc gene from 1371 samples collected at farms in Chongqing. The agar dilution method was used to determine the resistant phenotype, and extended spectrum β‐lactamase genes were amplified by PCR. Methicillin‐resistant S. aureus was verified by the presence of the mecA gene, and the presence or absence of SE, SEl, and TSST‐1 genes was detected in the isolates. Results We cultured 89 S. aureus isolates from 1371 samples between March 2014 and December 2017. These isolates were from pigs, cattle, goats, rabbits, and chickens. There were four methicillin‐resistant S. aureus strains (three from pigs and one from a chicken). The 89 isolates had high resistance to penicillin (93.3%) and ampicillin (92.1%), but most were susceptible to amikacin and ofloxacin, with resistance rates below 10%. A total of 62.9% of the isolates had varying degrees of multidrug resistance. Almost all strains, except for three isolates from chickens, were positive for bla TEM‐1a . There were 19 of 20 tested staphylococcal SE/SEl/TSST‐1 genes present (all except for seq ), and the predominant genes were sei (58.4%), tst‐1 (56.2%), and seg (51.7%). Conclusions The high antimicrobial resistance and prevalence of bla TEM‐1a reinforce the need to reduce the usage of antimicrobials in livestock. The universal existence of staphylococcal toxin genes implies a potential threat to public health by animal‐to‐human transmission via the food chain.

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Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China

Cited by 36 publications

References 32 publications

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Prevalence and characterization of Staphylococcus aureus from different processing points in beef processing plants in Shandong, China

Prevalence, antimicrobial resistance, and staphylococcal toxin genes of bla_TEM‐1a‐producing Staphylococcus aureus isolated from animals in Chongqing, China

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Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China

Cited by 36 publications

References 32 publications

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Prevalence and characterization of Staphylococcus aureus from different processing points in beef processing plants in Shandong, China

Prevalence, antimicrobial resistance, and staphylococcal toxin genes of blaTEM‐1a‐producing Staphylococcus aureus isolated from animals in Chongqing, China

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Product

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Prevalence, antimicrobial resistance, and staphylococcal toxin genes of bla_TEM‐1a‐producing Staphylococcus aureus isolated from animals in Chongqing, China