Antimicrobial resistant bacteria recovered from retail ground meat products in the US include a Raoultella ornithinolytica co-harboring blaKPC-2 and blaNDM-5

Abstract

Retail beef and pork, including processed products, can serve as vehicles for the zoonotic foodborne transmission of pathogens and antimicrobial resistant bacteria. However, processed and seasoned products like sausages, are not often included in research and surveillance programs. The objective of this study was to investigate retail ground beef and pork, including processed products, for the presence of common foodborne pathogens and antimicrobial resistant bacteria. We purchased 763 packages of fresh and fully cooked retail meat products during 29 visits to 17 grocery stores representing seven major grocery chains located in west and central Ohio. Each package of meat was evaluated for contamination with methicillin-resistant Staphylococcus aureus (MRSA), Salmonella spp., Enterobacteriaceae expressing extended-spectrum cephalosporin resistance, and carbapenemase-producing organisms (CPO). Only 3 of the 144 (2.1%) packages of fully cooked meat products contained any of these organisms, 1 with an extended-spectrum β-lactamase-producing (ESBL) Enterobacteriaceae and 2 with CPO. Among the 619 fresh meat products, we found that 85 (13.7%) packages were contaminated with MRSA, 19 (3.1%) with Salmonella, 136 (22.0%) with Enterobacteriaceae expressing an AmpC (blaCMY) resistance genotype, 25 (4.0%) with Enterobacteriaceae expressing an ESBL (blaCTX-M) resistance genotype, and 31 (5.0%) with CPO, primarily environmental organisms expressing intrinsic carbapenem resistance. However, one CPO, a Raoultella ornithinolytica, isolated from pork sausage co-harbored both blaKPC-2 and blaNDM-5 on IncN and IncX3 plasmids, respectively. Our findings suggest that fresh retail meat, including processed products can be important vehicles for the transmission of foodborne pathogens and antimicrobial resistant bacteria, including those with epidemic carbapenemase-producing genotypes.

Evaluation of canine raw food products for the presence of extended-spectrum beta-lactamase- and carbapenemase-producing bacteria of the order Enterobacterales

“…ESBL surveys of pork-based raw products for dogs have not been widely performed, but in a recent survey of ground raw pork and beef products intended for human consumption in the United States, ESBL-producing bacteria were more prevalent among pork products. 36 It is important to note that, within this study, ESBL organisms were isolated from products with a variety of protein sources including beef and poultry, which suggests that no products are without risks.…”

Antimicrobial Resistance Profile of Bacterial Isolates from Urinary Tract Infections in Companion Animals in Central Italy

“…The multidrug-resistant Raoultella ornithinolytica was recovered from canine urine samples in the present study. This bacterium was previously described in retail vegetables and meat [ 45 , 46 ], chicken products, poultry flock environments [ 47 , 48 ], and a septicemic calf [ 49 ]. Recently, it was isolated from feline urine in Austria, and it was evaluated as being MDR [ 50 ].…”

Improving the aroma profile of inoculated fermented sausages by constructing a synthetic core microbial community

“…Hence, the dominant genera were identified to be ubiquitously distributed, as they are present in all three spontaneously fermented sausages (SF 1 –SF 3 ). In several studies, the dominant genera were also identified in dry‐fermented sausages (Ballash et al., 2021; Juarez‐Castelan et al., 2019; Polka et al., 2015; Quijada et al., 2018; Zhang et al., 2021). The microbial diversity of the SF group was much higher than that of the IF 1–2 groups.…”

“…Hence, the dominant genera were identified to be ubiquitously distributed, as they are present in all three spontaneously fermented sausages (SF 1 -SF 3 ). In several studies, the dominant genera were also identified in dryfermented sausages (Ballash et al, 2021…”