Antimicrobial susceptibilities and bacteriological characteristics of bovine Pseudomonas aeruginosa and Serratia marcescens isolates from Mastitis

Ohnishi, Mamoru; Sawada, Takuo; Hirose, Kazuhiko; Sato, Reiichiro; Hayashimoto, Mizuki; Hata, Enjô; Yonezawa, Chizuko; Kato, Harubumi

doi:10.1016/j.vetmic.2011.06.023

Cited by 32 publications

(27 citation statements)

References 14 publications

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“…In Japan, S. marcescens strains isolated from milk were most often resistant to cefuroxime (100%), ampicillin (98.2%) and trimethoprim-sulfamethoxazole (96.6%) [15]. Most (98.3%) strains of S. marcescens were susceptible to amikacin, and all to gentamicin and ciprofloxacin [15]. In the current study, two isolates of L. monocytogenes were resistant, and one susceptible to all the studied antibiotics.…”

Section: Discussionsupporting

confidence: 42%

“…Similarly, in Sweden, strains of S. aureus, E. coli and K. pneumoniae isolated from milk showed susceptibility to the used antibiotics (among others, to gentamicin, ciprofloxacin, trimethoprim-sulfamethoxazole) [8,29]. In Japan, S. marcescens strains isolated from milk were most often resistant to cefuroxime (100%), ampicillin (98.2%) and trimethoprim-sulfamethoxazole (96.6%) [15]. Most (98.3%) strains of S. marcescens were susceptible to amikacin, and all to gentamicin and ciprofloxacin [15].…”

Section: Discussionmentioning

confidence: 98%

“…Klebsiella pneumoniae and Serratia marcescens are rarely isolated from cases of mastitis; however, infections caused by these bacteria are difficult to treat [14]. In Poland and in Japan, the incidence of S. marcescens in cows with mastitis is 3.1% and 4.7%, respectively [10,15].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle

Skowron¹,

Sękowska²,

Kaczmarek³

et al. 2019

Ann Agric Environ Med.

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Introduction. Mastitis may result in physical, chemical and microbiological changes in milk and pathological lesions in the glandular tissue. Milk derived from cows with mastitis may become a cause of infections in humansw and animals. Objectives. The aim of this study was to assess the effectiveness of selected dipping agents in the inactivation of several bacteria that may cause mastitis in cattle. Materials and method. Three strains of each of the following species: Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Staphylococcus aureus and Listeria monocytogenes, isolated from milk, were used in the study. Identification of isolates was carried out using the automatic system VITEK2 Compact. Evaluation of the genetic similarity between the tested strains was made using the RAPD technique. Drug susceptibility of strains was evaluated with the disc diffusion method. Assessment of the effectiveness of iodine, stabilized iodine, povidone iodine and chlorhexidine was performed using fragments of skin from cow teats. Results. All the tested strains were genetically different. Most of them were susceptible to the studied antibiotics. Only two strains of L. monocytogenes were resistant to all the studied antibiotics. The percentage rate of reduction in the number of bacteria after using of dipping agents was very high (>90%). The most susceptible to the dipping preparations used were L. monocytogenes (99.6-99.9%). Stabilized iodine was the most effective dipping agent for all tested bacteria, causing a reduction rate in the number of bacteria from 99.80% (E. coli)-99.99% (S. aureus, L. monocytogenes). Conclusions. The results obtained may contribute to a reduction in udder infections in cows, especially mastitis, and improve the quality of the milk.

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Section: Discussionsupporting

confidence: 42%

Section: Discussionmentioning

confidence: 98%

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Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle

Skowron¹,

Sękowska²,

Kaczmarek³

et al. 2019

Ann Agric Environ Med.

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“…venerealis culture media but P. aeruginosa strains are increasingly becoming resistant to multiple classes of antibiotics and strains exhibiting a multiple resistance phenotype are emerging in humans, accentuating the fact that the results obtained here may be strain-specific (Gales, 2001). Indeed, 4.3% of previously evaluated bovine P. aeruginosa strains (n=116) exhibited resistance to gentamicin (Ohnishi et al, 2011) while a recent German study found that all C. fetus subsp. venerealis isolates tested (n=50) were susceptible to gentamicin, although this antibiotic is included in both Clark's and modified…”

Section: Discussionmentioning

confidence: 67%

“…Antimicrobial sensitivity testing of 116 bovine P. aeruginosa isolates showed that the majority of strains were susceptible to amikacin, cefepime, cefoperazone/sulbactam, ceftazidime, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin and tobramycin (Ohnishi et al, 2011). Such antibiotics could potentially be added to C. fetus subsp.…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostic protocols for bovine genital campylobacteriosis using comparative genomics and virulence studies

Indjein¹

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Bovine genital campylobacteriosis is difficult to identify due to problems with the isolation of its causative agent Campylobacter fetus subspecies venerealis. To optimise isolation methods, three selective culture and three transport and enrichment media (TEMs) were evaluated using a pure C. fetus subsp. venerealis culture, with and without simulated field contamination by Pseudomonas aeruginosa. For optimal C. fetus subsp. venerealis isolation and growth, TEMs should be returned from the field within 48h of collection and plated within 4h onto Campylobacter selective agar plates incubated for 48-72h at 37°C under microaerophilic conditions. Longer transport or TEM incubation resulted in overgrowth of P. aeruginosa limiting C. fetus subsp. venerealis recovery.In order to establish an isolate collection, the parA real-time PCR assay was used to screen bull preputial secretions prior to culturing positive samples. A more sensitive real-time PCR assay was designed from the ISCfe1 insertion element and used to assess additional preputial samples, compared to the parA real-time assay. A proportion of real-time negative samples was also cultured. Fourteen cultures were obtained from either ISCfe1 (8/43; 18.6%) or parA (6/51; 11.8%) negative samples. ISCfe1 and parA sequences were obtained from isolates not confirmed by conventional methods suggesting that both parA and ISCfe1 may be found in organisms other than C. fetus subsp. venerealis. Molecular testing of cultures (n=84) confirmed the presence of these sequences in isolates identified as other Campylobacter spp. or Arcobacter-like organisms, while they were not always detected in all biochemically confirmed C. fetus subsp. venerealis isolates. The lack of specificity of all methods highlights the need for more reliable C. fetus subsp. venerealis-specific gene targets.The draft genomes of two C. fetus subsp. venerealis biovars aligned to C. fetus subsp. fetus genome provided 26 unique C. fetus subsp. venerealis regions. In order to identify new diagnostic targets, 13 putative conventional and one real-time PCR assays designed from contigs common to both C. fetus subsp. venerealis biovars were developed to screen the Campylobacter-like culture collection (n=84). Isolates biochemically identified as organisms other than C. fetus subsp.venerealis were positive on the new real-time and six conventional PCR assays, indicating that they were not suitable subspecies-specific markers. Seven assays detected isolates phenotyped as C. fetus subsp. venerealis except for two aerotolerant isolates recognized as C. fetus subsp. venerealis by iii other published methods. These seven gene targets could be further investigated for real-time PCR assay development as in silico BLAST analysis confirmed their conservation in the genomes of five of our C. fetus subsp. venerealis isolates. However, further bioinformatics analysis revealed that they were found in several C. fetus subsp. fetus strains, underlining that a pan-genomics study including multiple C. fetus genomes is ne...

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Molecular Biology Perspective of Susceptibility and Resistance in Main Target Pathogens in the Respective Species and Antimicrobials of Concern

Pokludová

2020

Antimicrobials in Livestock 1: Regulation, Science, Practice

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Antimicrobial susceptibilities and bacteriological characteristics of bovine Pseudomonas aeruginosa and Serratia marcescens isolates from Mastitis

Cited by 32 publications

References 14 publications

Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle

Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle

Molecular diagnostic protocols for bovine genital campylobacteriosis using comparative genomics and virulence studies

Molecular Biology Perspective of Susceptibility and Resistance in Main Target Pathogens in the Respective Species and Antimicrobials of Concern

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