Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers

Kocúreková, Tímea; Karahutová, Lívia; Bujňáková, Dobroslava

doi:10.3390/antibiotics10111303

Cited by 5 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). The results agree with those of Kocúreková et al [16] in Slovakia who found Tobramycin E. coli resistance from broilers in 1.96%. The most common mechanism of acquired resistance to aminoglycosides is the modification of the chemical structure of the antibiotic by bacterial enzymes.…”

Section: Tobramycin E Coli Resistance Before Covid-19 Appearancesupporting

confidence: 92%

“…Animal products may contain antimicrobial resistant bacteria as a result of fecal contamination during slaughter [32]. Commercial broilers can be reservoirs of virulent and resistant genes as well as Escherichia coli causing extra-intestinal infections, which can be a potential threat to humans via direct contact and food [16]. During the processing of meat, the bacteria from animal origin can contaminate other food items, the processing plant, or workers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Guergueb

Alloui

2023

RC FCV-LUZ

View full text Add to dashboard Cite

The effect of excessive use of biocides during the COVID-19, on the resistance of Escherichia coli to Tobramycin in poultry, meat was examined in this observational epidemiological study (Before and after COVID–19). Tobramycin E. coli resistant strains isolated from poultry meat before COVID-19 appearance were compared with those isolated after COVID-19 emergence. Univariable analyses were performed using t-test and chi-squared test. Odds ratios and 95% confidence intervals were used for statistically significant risk factor. Multivariate analysis was done with the binary logistic regression to detect an independent predictor, and with the principal component analysis (PCA), to analyze whether the Tobramycin resistance in E. coli was linked with the COVID-19 outbreak. Statistical significance was set at P<0.05. The frequency of Tobramycin E. coli resistant isolates was more important after COVID-19 emergence (12.5%) than before COVID-19 (2.1%). Graphical representation of PCA qualitative variables shows the interfactor relationship. A significant relationship between Tobramycin E. coli resistance and COVID-19 emergence (P=0.014), and the effect of the emergence of COVID-19 on the Tobramycin E. coli resistance was OR = 6.57 (95% Confidence interval (CI) 1.61-7.94). The probability of Tobramycin E. coli resistance linked with poultry meat bought after COVID-19 was 1.88 times more than before COVID-19 emergence. Poultry meat purchased after COVID-19 found related to Tobramycin resistance in E. coli. It seems possible that the overuse of biocides during COVID-19 increased the risk of Tobramycin E. coli resistance in poultry meat.

show abstract

Section: Tobramycin E Coli Resistance Before Covid-19 Appearancesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Guergueb

Alloui

2023

RC FCV-LUZ

View full text Add to dashboard Cite

show abstract

Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian Pathogenic Escherichia coli

Koratkar,

Bhutada,

Giram

et al. 2024

PHAGE

View full text Add to dashboard Cite

Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat

Dančová,

Gregová,

Szabóová

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

Escherichia coli isolates from intensive poultry production are associated with antimicrobial resistance and worldwide health problems. The aim of the study was to detect and evaluate the phenotypic and genotypic antimicrobial resistance, biofilm formation, phylogenetic typing, and virulence factors in E. coli isolates from the rectal swabs of chickens from two farms and swabs of chicken meat purchased from Slovakian food markets. Interpretative readings of minimal inhibitory concentration (MIC) revealed dominant resistance to ampicillin (>50%) in both groups. We also detected higher resistance to ciprofloxacin (45%), tetracycline, ampicillin + sulbactam, and trimethoprim + sulfonamide (each >30%). Here, 28.57% of the strains studied were multidrug-resistant (MDR). The formation of weak biofilms was confirmed in 8.8% of E. coli, while one of the strains obtained from chicken cloacal swabs was classified as a strong biofilm producer. The most frequently confirmed phylogenetic groups in E. coli were B1 and A1 in all groups. PCR detection revealed the presence of genes encoding tetracycline resistance (tetAB) and plasmid-mediated quinolone resistance (qnrABS), and Int1 (52.9%), Tn3 (76.5%), kpsMT II (8.8%), fimA (97.1%), cvaC (38.2%), and iutA (76.5%) genes in the strains studied. Our results demonstrate that chickens and chicken meat were the source of antibiotic-resistant, biofilm-forming, and virulent E. coli, representing a potential risk from the point of view of the One Health concept.

show abstract

Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers

Cited by 5 publications

References 60 publications

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian Pathogenic Escherichia coli

Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat

Contact Info

Product

Resources

About