Antimicrobial susceptibility and tetracycline resistance determinant genotyping of Gallibacterium anatis

Bojesen, Anders Miki; Vázquez, María Elena Sánchez del Río; Bager, Ragnhild J.; Ifrah, Dan; González, Carlos; Aarestrup, Frank Møller

doi:10.1016/j.vetmic.2010.08.011

Cited by 40 publications

(43 citation statements)

References 17 publications

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“…De igual manera un tratamiento eficaz con antibióticos específicos permitiría mantener altos niveles de salud animal. Sin embargo, en México, los productores de aves, suelen fracasar en el tratamiento con antibióticos utilizados contra la infección de G. anatis (15) , debido a que los organismos pertenecientes a la familia de las Pasteurellaceae muestran resistencia recurrente debido al uso desmedido de antibióticos no específicos (13) . Es por esto, que el objetivo del presente trabajo fue determinar la susceptibilidad y resistencia a diferentes antibióticos, incluyendo los más utilizados para el tratamiento de G. anatis.…”

Section: Se Consiguieron Identificar 23 Aislamientos De Gallibacteriuunclassified

“…La patogenia de G. anatis está poco definida, por lo que existen pocos estudios que expliquen la farmacocinética de este microorganismo, lo que ha inclinado al productor a medicar desmedidamente esta enfermedad, ocasionando que la bacteria desarrolle una resistencia marcada, como se ha demostrado (30,31) , al comparar los fenotipos de resistencia con los informes de otros taxones de Pasteurellaceae, encontrándose una alta multirresistencia de las tetraciclinas y sulfametoxazol del 92 y 97 % en los aislamientos de campo de Gallibacterium. Confirmando así los resultados obtenidos en el presente estudio, donde la mayoría de los aislamientos presentaron resistencia marcada con las tetraciclinas y los betalactámicos.…”

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“…Sin embargo, aunque la resistencia parezca ser muy común entre los aislamientos de G. anatis, los que no son de campo, muestran menor resistencia a algunos antibióticos (30) .…”

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Antimicrobial resistance of Gallibacterium anatis isolates from breeding and laying commercial hens in Sonora, Mexico

Chávez

Barrios

Xóchihua

et al. 2017

Rev. Mex. Cienc. Pecu.

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RESUMENSe consiguieron identificar 23 aislamientos de Gallibacterium anatis, a través de pruebas convencionales de cultivo y bioquímicas, confirmándose su identificación a través del método de PCR. Se les efectuó la prueba de difusión en placa para determinar la resistencia antimicrobiana a los antibióticos más utilizados en el área avícola. Los aislamientos fueron más susceptibles a ceftiofur (73 %) y florfenicol (68 %); todos los aislamientos mostraron resistencia marcada a penicilina, tilosina, lincomicina, ampicilina, enrofloxacina, oxitetraciclina, norfloxacina y cefalexina. La presencia de Gallibacterium anatis se asocia a problemas respiratorios y reproductivos en las poblaciones de gallinas de postura comercial. Los aislamientos mostraron una resistencia marcada a distintos antibióticos, probablemente por la medicación desmedida ante la presencia de este microorganismo. PALABRAS CLAVE: Gallibacterium anatis, PCR, Resistencia, Susceptibilidad, Antimicrobianos. ABSTRACTIt was able to identify 23 isolates of Gallibacterium anatis through conventional culture and biochemical tests, and identification confirmed through PCR method. A disk diffusion test determined antimicrobial resistance to commonly used antibiotics in the poultry area. Isolates were more susceptible to ceftiofur (73 %) and florfenicol (68 %). All isolates showed marked resistance to penicillin, tylosin, lincomycin, ampicillin, enrofloxacin, oxytetracycline, norfloxacin and cephalexin. The presence of Gallibacterium anatis is associated with respiratory and reproductive problems in populations of commercial laying hens. Isolates showed marked resistance to different antibiotics, probably due to excessive medication in the presence of this organism.

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Section: Se Consiguieron Identificar 23 Aislamientos De Gallibacteriuunclassified

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Antimicrobial resistance of Gallibacterium anatis isolates from breeding and laying commercial hens in Sonora, Mexico

Chávez

Barrios

Xóchihua

et al. 2017

Rev. Mex. Cienc. Pecu.

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“…Besides constituting a part of the normal microflora of the upper respiratory tract and lower genital tract in chickens (4)(5)(6), Gallibacterium anatis has recently been recognized as a major cause of lesions in the reproductive tracts of egg layers (7)(8)(9)(10), causing a drop in egg production and increased mortality (11). Multiple-drug resistance (12) and a substantial antigenic diversity (13) make it difficult to prevent the negative effects of G. anatis using traditional antimicrobial agents and vaccines. Thus, novel prevention and treatment strategies are urgently needed.…”

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The Fimbrial Protein FlfA from Gallibacterium anatis Is a Virulence Factor and Vaccine Candidate

et al. 2013

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cThe Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in egg-laying chickens, leading to decreased egg production worldwide. Widespread multidrug resistance largely prevents treatment of this organism using traditional antimicrobial agents, while antigenic diversity hampers disease prevention by classical vaccines. Thus, insight into its pathogenesis and knowledge about important virulence factors is urgently required. A key event during the colonization and invasion of mucosal surfaces is adherence, and recently, at least three F17-like fimbrial gene clusters were identified in the genomes of several G. anatis strains. The objective of this study was to characterize the putative F17-like fimbrial subunit protein FlfA from G. anatis 12656-12 and determine its importance for virulence. In vitro expression and surface exposure of FlfA was demonstrated by flow cytometry and immunofluorescence microscopy. The predicted function of FlfA as a fimbrial subunit protein was confirmed by immunogold electron microscopy. An flfA deletion mutant (⌬flfA) was generated in G. anatis 12656-12, and importantly, this mutant was significantly attenuated in the natural chicken host. Furthermore, protection against G. anatis 12656-12 could be induced by immunizing chickens with recombinant FlfA. Finally, in vitro expression of FlfA homologs was observed in a genetically diverse set of G. anatis strains, suggesting the potential of FlfA as a serotype-independent vaccine candidate This is the first study describing a fimbrial subunit protein of G. anatis with a clear potential as a vaccine antigen.

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“…Thus, G. anatis infections lead to a drop in egg production and increased mortality in commercial layers [10]. Unfortunately, widespread multiple-drug resistance [11] hinders treatment with traditional antimicrobial agents, while substantial antigenic diversity [12] among disease-causing field isolates hampers disease prevention by classical vaccination with inactivated whole cell bacterins. Hence, novel prevention strategies are urgently needed.…”

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confidence: 99%

In silico prediction of

Bager

Kudirkienė

Piedade

et al. 2014

Vet Res

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The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309.

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Antimicrobial susceptibility and tetracycline resistance determinant genotyping of Gallibacterium anatis

Cited by 40 publications

References 17 publications

Antimicrobial resistance of Gallibacterium anatis isolates from breeding and laying commercial hens in Sonora, Mexico

Antimicrobial resistance of Gallibacterium anatis isolates from breeding and laying commercial hens in Sonora, Mexico

The Fimbrial Protein FlfA from Gallibacterium anatis Is a Virulence Factor and Vaccine Candidate

In silico prediction of

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