Antimicrobial susceptibility, biofilm formation and genetic profiles of Escherichia coli isolated from retail chicken meat

Crecencio, Regiane Boaretto; Brisola, Maiara Cristiane; Bitner, Dinael Simão; Frigo, Angélica; Rampazzo, Luana; Borges, Karen Apellanis; Furian, Thales Quedi; Salle, Carlos Tadeu Pippi; Moraes, H. L. S.; Faria, Gláucia Amorim; Silva, Aleksandro S. Da; Stefani, Lenita M.

doi:10.1016/j.meegid.2020.104355

Cited by 28 publications

(23 citation statements)

References 32 publications

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“…In the present study, the highest resistance profile was observed for ampicillin (100%), followed by sulfamethoxazole and tetracycline (88.24% for both), and trimethoprim and chloramphenicol (82.35% ex aequo ); this finding is in accordance with other studies conducted in Brazil by Crecencio et al [ 58 ] and in Portugal by Manageiro et al [ 59 ]. Manageiro et al revealed that a high percentage of E. coli strains isolated from broilers and turkeys were resistant to the following antimicrobial agents: ciprofloxacin (90.6% and 79.5%), nalidixic acid (88.6% and 73.5%), ampicillin (75.7% and 80%), sulfamethoxazole (69.3% and 71.9%), tetracycline (66.3% and 85.9%), trimethoprim (54.5% and 49.7%), and chloramphenicol (34.2% and 52.4%).…”

Section: Discussionsupporting

confidence: 93%

“…Interestingly, multidrug resistance was observed in 81.3% of the isolates. Crecencio et al [ 58 ] showed the highest resistance of retail chicken meat to beta-lactams (39.5%), followed by sulfonamide combined with trimethoprim (36.9%) and polymyxin (33.4%).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, 71% of E. coli strains isolated from chicken exhibited weak and medium biofilm production ability, which is in agreement with the results of our present study. In comparison, Crecencio et al [ 58 ] showed that as many as 70.44% of E. coli strains isolated from retail chicken meat had moderate to strong biofilm formation ability. These discrepancies may depend on strain properties, culture conditions, environmental factors, and methodology [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

et al. 2021

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Background A plasmid-mediated mechanism of bacterial resistance to polymyxin is a serious threat to public health worldwide. The present study aimed to determine the occurrence of plasmid-mediated colistin resistance genes and to conduct the molecular characterization of mcr-positive Escherichia coli strains isolated from Polish poultry. Methods In this study, 318 E. coli strains were characterized by the prevalence of mcr1–mcr5 genes, antimicrobial susceptibility testing by minimal inhibitory concentration method, the presence of antimicrobial resistance genes was screened by PCR, and the biofilm formation ability was tested using the crystal violet staining method. Genetic relatedness of mcr-1-positive E. coli strains was evaluated by multilocus sequence typing method. Results Among the 318 E. coli isolates, 17 (5.35%) harbored the mcr-1 gene. High antimicrobial resistance rates were observed for ampicillin (100%), tetracycline (88.24%), and chloramphenicol (82.35%). All mcr-1-positive E. coli strains were multidrug-resistant, and as many as 88.24% of the isolates contained the blaTEM gene, tetracycline (tetA and tetB), and sulfonamide (sul1, sul2, and sul3) resistance genes. Additionally, 41.18% of multidrug-resistant, mcr-1-positive E. coli isolates were moderate biofilm producers, while the rest of the strains showed weak biofilm production. Nine different sequence types were identified, and the dominant ST was ST93 (29.41%), followed by ST117 (17.65%), ST156 (11.76%), ST 8979 (11.76%), ST744 (5.88%), and ST10 (5.88%). Moreover, the new ST was identified in this study. Conclusions Our results showed a low occurrence of mcr-1-positive E. coli strains isolated from Polish poultry; however, all the isolated strains were resistant to multiple antimicrobial agents and were able to form biofilms at low or medium level.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

et al. 2021

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“…These species are representative of food-poisoning pathogens detected in poultry meat. In addition, they can form bacterial biofilms, which are a source of antimicrobial resistance [ 27 , 28 , 29 , 30 ]. When S .…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and Antibiofilm Effect of ε-Polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in Tryptic Soy Broth and Chicken Juice

Lee

Park

et al. 2021

Foods

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ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (Salmonella, Listeria, E. coli, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing.

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“…The CDC estimates that Salmonella causes approximately 1.2 million diseases and 450 deaths each year in the United States and reports that it is the most common cause of food poisoning in June, July, and August [2,23]. As noted in many studies, E. coli [11,[24][25][26] and S. typhimurium [27][28][29][30][31][32] form a strong biofilm on the surface of non-living objects or living organisms. Cross-contamination in agro-food processing lines caused by biofilms is a serious concern in the food industry.…”

Section: Introductionmentioning

confidence: 99%

Detecting Bacterial Biofilms Using Fluorescence Hyperspectral Imaging and Various Discriminant Analyses

Lee

Park

Yoo

et al. 2021

Sensors

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Biofilms formed on the surface of agro-food processing facilities can cause food poisoning by providing an environment in which bacteria can be cultured. Therefore, hygiene management through initial detection is important. This study aimed to assess the feasibility of detecting Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium) on the surface of food processing facilities by using fluorescence hyperspectral imaging. E. coli and S. typhimurium were cultured on high-density polyethylene and stainless steel coupons, which are the main materials used in food processing facilities. We obtained fluorescence hyperspectral images for the range of 420–730 nm by emitting UV light from a 365 nm UV light source. The images were used to perform discriminant analyses (linear discriminant analysis, k-nearest neighbor analysis, and partial-least squares discriminant analysis) to identify and classify coupons on which bacteria could be cultured. The discriminant performances of specificity and sensitivity for E. coli (1–4 log CFU·cm−2) and S. typhimurium (1–6 log CFU·cm−2) were over 90% for most machine learning models used, and the highest performances were generally obtained from the k-nearest neighbor (k-NN) model. The application of the learning model to the hyperspectral image confirmed that the biofilm detection was well performed. This result indicates the possibility of rapidly inspecting biofilms using fluorescence hyperspectral images.

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Antimicrobial susceptibility, biofilm formation and genetic profiles of Escherichia coli isolated from retail chicken meat

Cited by 28 publications

References 32 publications

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

Antimicrobial and Antibiofilm Effect of ε-Polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in Tryptic Soy Broth and Chicken Juice

Detecting Bacterial Biofilms Using Fluorescence Hyperspectral Imaging and Various Discriminant Analyses

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