Antimicrobial susceptibility monitoring of Mycoplasma hyopneumoniae and Mycoplasma bovis isolated in Europe

Klein, U.; Jong, Anno de; Moyaert, Hilde; Garch, Farid El; Leon, Rocio; Richard‐Mazet, Alexandra; Rose, Markus; Maes, Dominiek; Pridmore, Andrew; Thomson, Jill R.; Ayling, Roger D.

doi:10.1016/j.vetmic.2017.04.012

Cited by 44 publications

(63 citation statements)

References 33 publications

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“…Also, Andersen and others [25], described the presence of alternative bacterial resistance mechanisms for tylosin in comparison to tylvalosin, which can explain the differences in MIC values between members of the 16membered ring class of macrolides. The tylosin and tilmicosin MIC values were in agreement with those found in M. hyosynoviae [10], M. hyorhinis [12] and M. hyopneumoniae [17,23]. Based on our data, the macrolides could still be utilised as first-line antimicrobial treatment for the control of mycoplasma disease in pigs.…”

Section: Discussionsupporting

confidence: 85%

“…Felde et al [22] described tulathromycin MIC 90 values of 1 and 4 μg/ml, 1-4 two-fold dilutions higher to those observed for tylvalosin after analysing Central European M. hyopneumoniae isolates. Conversely, Klein et al [23] described MIC 90 values for tulathromycin of 0.004 μg/ml, after analysing 50 European M. hyopneumoniae isolates, however, the authors did not include tylvalosin in their analysis. In addition, decreased susceptibility to tylosin and tilmicosin has been previously described in European M. hyosynoviae and M. hyopneumoniae isolates [10,24].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

et al. 2020

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Background: Mycoplasma (M.) hyopneumoniae, M. hyorhinis and M. hyosynoviae are significant pathogens for the porcine industry worldwide. The aim of the present study was to determine the antimicrobial susceptibility of six key antimicrobials (tylosin, tilmicosin, tylvalosin, lincomycin, tiamulin and valnemulin) routinely used for treating infections caused by these pathogens. Twenty-seven M. hyopneumoniae, 48 M. hyorhinis and 40 M. hyosynoviae field strains isolated from clinical samples from different Southern European countries between 2013 and 2018 using broth microdilution method were evaluated. Results: Tylvalosin exhibited the highest in vitro activity among the macrolides assayed, with MIC 90 values 4 to 5 twofold dilutions lower than those of tylosin and tilmicosin. The pleuromutilin valnemulin showed one of the highest in vitro activities against the three mycoplasma species. On the contrary, lincomycin exhibited the highest MIC values of the antimicrobials tested. Conclusions: The data obtained in the present study supports the use of pleuromutilins and macrolides for the control of infections caused by porcine mycoplasmas. The use of lincomycin for the treatment of porcine mycoplasma infections should be carefully evaluated due to the presence of circulating field isolates with decreased susceptibility to this antimicrobial.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

et al. 2020

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“…The susceptibility to valnemulin and tiamulin may have decreased in the period 1997 (Hannan et al, 1997) to 2013 (Tavio et al, 2014;Thongkamkoon et al, 2013). However, taken together, acquired antimicrobial resistance does currently not seem to constitute a major problem for treatment of M. hyopneumoniae infections (Klein et al, 2017), although the situation may be different for other bacteria complicating these infections. Finally, within a farm, strains with differences in antibiotic susceptibility may coexist (Thongkamkoon et al, 2013).…”

Section: Therapeuticsmentioning

confidence: 99%

Update onMycoplasma hyopneumoniaeinfections in pigs: Knowledge gaps for improved disease control

Maes

Sibila

Kuhnert

et al. 2017

Transbound Emerg Dis

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SummaryMycoplasma hyopneumoniae (M. hyopneumoniae) is the primary pathogen of enzootic pneumonia, a chronic respiratory disease in pigs. Infections occur worldwide and cause major economic losses to the pig industry. The present paper reviews the current knowledge on M. hyopneumoniae infections, with emphasis on identification and analysis of knowledge gaps for optimizing control of the disease. Close contact between infected and susceptible pigs is the main route of M. hyopneumoniae transmission. Management and housing conditions predisposing for infection or disease are known, but further research is needed to better understand M. hyopneumoniae transmission patterns in modern pig production systems, and to assess the impor-

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“…Mycoplasma hyopneumoniae is intrinsically resistant to antibiotics which interfere with the polymerization of cell wall precursors, such as beta‐lactam antibiotics and to polymyxins, 14‐membered ring macrolides (such as oleandomycin and erythromycin), trimethoprim and sulfonamides. In vitro , acquired resistance has been documented for tetracyclines, 15‐membered ring macrolides (tulathromycin, gamithromycin), 16‐membered ring macrolides (tylosin, tilmicosin), lincosamides (lincomycin) and fluoroquinolones (Felde, Kreizinger, Sulyok, Hrivnak, et al, ; Felde, Kreizinger, Sulyok, Marton, et al, ; Klein et al, ; Tavio, Poveda, Assunção, Ramírez, & Poveda, ; Vicca et al, ; Vicca et al, ). It was shown that mutations in the 23S rRNA gene were responsible for resistance to macrolides and lincosamides (Stakenborg et al, ).…”

Section: Mycoplasma Hyopneumoniae Variabilitymentioning

confidence: 99%

Mycoplasma hyopneumoniaevariability:Current trends and proposed terminology for genomic classification

Betlach

Maes

Garza-Moreno

et al. 2019

Transbound Emerg Dis

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Mycoplasma hyopneumoniae (M. hyopneumoniae) is the aetiologic agent of enzootic pneumonia in swine, a prevalent chronic respiratory disease worldwide. Mycoplasma hyopneumoniae is a small, self-replicating microorganism that possesses several characteristics allowing for limited biosynthetic abilities, resulting in the fastidious, hostspecific growth and unique pathogenic properties of this microorganism. Variation across several isolates of M. hyopneumoniae has been described at antigenic, proteomic, transcriptomic, pathogenic and genomic levels. The microorganism possesses a minimal number of genes that regulate the transcription process. Post-translational modifications (PTM) occur frequently in a wide range of functional proteins. The PTM by which M. hyopneumoniae regulates its surface topography could play key roles in cell adhesion, evasion and/or modulation of the host immune system. The clinical outcome of M. hyopneumoniae infections is determined by different factors, such as housing conditions, management practices, co-infections and also by virulence differences among M. hyopneumoniae isolates. Factors contributing to adherence and colonization as well as the capacity to modulate inflammatory and immune responses might be crucial. Different components of the cell membrane (i.e. proteins, glycoproteins and lipoproteins) may serve as adhesins and/or be toxic for the respiratory tract cells. Mechanisms leading to virulence are complex and more research is needed to identify markers for virulence. The utilization of typing methods and complete or partial-gene sequencing for M. hyopneumoniae characterization has increased in diagnostic laboratories as control and elimination strategies for this microorganism are attempted worldwide. A commonly employed molecular typing method for M. hyopneumoniae is Multiple-Locus Variable number tandem repeat Analysis (MLVA). The agreement of a shared terminology and classification for the various techniques, specifically MLVA, has not been described, which makes inferences across the literature unsuitable. Therefore, molecular trends for M. hyopneumoniae have been outlined and a common terminology and classification based on Variable Number Tandem Repeats (VNTR) types has been proposed. K E Y W O R D S genomic classification, Mycoplasma hyopneumoniae, terminology, variability, Variable Number Tandem Repeats | 1841 BETLACH ET AL.

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Antimicrobial susceptibility monitoring of Mycoplasma hyopneumoniae and Mycoplasma bovis isolated in Europe

Cited by 44 publications

References 33 publications

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

Update onMycoplasma hyopneumoniaeinfections in pigs: Knowledge gaps for improved disease control

Mycoplasma hyopneumoniaevariability:Current trends and proposed terminology for genomic classification

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