Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Pigs with Respiratory Diseases on Farms in China

Zhao, Zhanqin; Xue, Yue; Wang, Chen; Ding, Ke; Wu, Bin; He, Qigai; Xiao, Cheng; Chen, Huanchun

doi:10.1292/jvms.10-0184

Cited by 14 publications

(18 citation statements)

References 13 publications

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“…Results from the Australian study and from other studies confirmed our observation of a broad distribution of B . bronchiseptica MIC values for trimethoprim/sulfamethoxazole and generally high MICs for β-lactam antibiotics [ 7 , 18 , 20 – 21 , 23 , 25 – 26 , 28 , 34 , 49 ]. A former study from Germany investigated MICs of isolates collected about 10 years before (between 2000 and 2003) and presented MIC 50 and MIC 90 values similar to MICs of this study, except for ceftiofur, neomycin, tetracycline, streptomycin and trimethoprim/sulfamethoxazole for which the MIC 90 values seem to have increased by two dilution steps over recent years [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

et al. 2015

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Bordetella bronchiseptica causes infections of the respiratory tract in swine and other mammals and is a precursor for secondary infections with Pasteurella multocida. Treatment of B. bronchiseptica infections is conducted primarily with antimicrobial agents. Therefore it is essential to get an overview of the susceptibility status of these bacteria. The aim of this study was to comparatively analyse broth microdilution susceptibility testing according to CLSI recommendations with an incubation time of 16 to 20 hours and a longer incubation time of 24 hours, as recently proposed to obtain more homogenous MICs. Susceptibility testing against a panel of 22 antimicrobial agents and two fixed combinations was performed with 107 porcine isolates from different farms and regions in Germany and 43 isolates obtained from companion animals in Germany and other European countries. Isolates with increased MICs were investigated by PCR assays for the presence of resistance genes. For ampicillin, all 107 porcine isolates were classified as resistant, whereas only a single isolate was resistant to florfenicol. All isolates obtained from companion animals showed elevated MICs for β-lactam antibiotics and demonstrated an overall low susceptibility to cephalosporines. Extension of the incubation time resulted in 1–2 dilution steps higher MIC50 values of porcine isolates for seven antimicrobial agents tested, while isolates from companion animals exhibited twofold higher MIC50/90 values only for tetracycline and cefotaxime. For three antimicrobial agents, lower MIC50 and MIC90 values were detected for both, porcine and companion animal isolates. Among the 150 isolates tested, the resistance genes bla BOR-1 (n = 147), bla OXA-2, (n = 4), strA and strB (n = 17), sul1 (n = 10), sul2 (n = 73), dfrA7 (n = 3) and tet(A) (n = 8) were detected and a plasmid localisation was identified for several of the resistance genes.

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Section: Discussionmentioning

confidence: 99%

“…bronchiseptica isolates from swine or companion animals in Europe or other countries [ 7 , 18 – 22 ]. A comparison between results from these studies can not be reliably performed due to the different methods of susceptibility testing used [ 22 – 25 ]. However, decreased antimicrobial susceptibility of B .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

et al. 2015

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“…Due to variation in antibiogram of isolates from different places no uniform antibiotic regimen may be recommended for treatment of bordetellosis in pigs. In china, Zhao et al [25] reported similar variation (~40 antibiogram types) in antibiotic sensitivity pattern among B. bronchiseptica isolates from healthy pigs of different provinces.…”

Section: Resultsmentioning

confidence: 99%

Occurrence ofBordetellaInfection in Pigs in Northern India

Kumar

Singh

Bhardwaj

et al. 2014

International Journal of Microbiology

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Bordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries. In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile. The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively. Observations revealed that there may be other bordetellae infecting pigs because about 50% of the samples positive using mPCR for genus specific amplicons failed to confirm presence of B. bronchiseptica. Of the pig sera tested with MAT and ELISA for Bordetella antibodies, 67.6% and 86.3% samples, respectively, were positive. For antigen detection mPCR was more sensitive than conventional bacterioscopy while for detection of antibodies neither of the two tests (MAT and ELISA) had specificity in relation to antigen detection. Study indicated high prevalence of infection in swine herds in Northern India.

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“…Of note, no clear guidelines are available currently for human infection, however, various agents including b-lactam/b-lactamase inhibitor combinations, carbapenems, tetracycline, and fluouroquinolones have been used successfully. In the patient reported by Dlamini et al [9] been shown to be susceptible to a variety of agents including tetracycline, fluouroquinolones, and b-lactam antibiotics [21,22]. Trimethoprim as well as sulfonamides are considered ineffective.…”

Section: Discussionmentioning

confidence: 99%

Bordetella bronchisepticaPneumonia Post-Liver Resection in a Patient with Liver Cirrhosis and Cholangiocarcinoma

BonattiHugo

CraigBrian

TheckumparampilNithin

et al. 2016

Surgical Infections Case Reports

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Background: Although Bordetella bronchiseptica is primarily an animal pathogen, cases of human disease caused by this pathogen have been published recently, most frequently pneumonia in immunocompromised patients. In human disease, transmission through animal vectors may play a key role. Although no standardized sensitivity testing is available for this pathogen in human disease, animal isolates are sensitive to most b-lactam antibiotics. Case Report: A 62-year-old Caucasian male with Child-Pugh class A cirrhosis caused by chronic hepatitis C infection underwent uneventful left lateral segmentectomy for a 3 cm cholangiocarcinoma. Within 48 h, he developed altered mental status, temperature of 39.4°C, leukocytosis (white blood cell count: 13,000/mm 3), and dyspnea followed by hypotension requiring vasopressor support and intubation. Computed tomography (CT) scan demonstrated left lower lobe pneumonia. Empiric antibiotic therapy including vancomycin (1 g every 12 h) and piperacillin-tazobactam (3.5 g every 6 h) was initiated and his signs of sepsis resolved within two days. Bordetella bronchiseptica was cultured from sputum. Upon questioning, the patient reported close contact with several pet cats on the days prior to admission. Antibiotics were continued for a total of seven days and he was discharged in good condition doing well at his six-month follow-up. Conclusions: Immunocompromised patients may develop infection with Bordetella bronchiseptica especially if they are in close contact with animals known to be a reservoir of this pathogen. If diagnosed early and treated appropriately, the outcome is favorable.

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Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Pigs with Respiratory Diseases on Farms in China

Cited by 14 publications

References 13 publications

Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

Occurrence ofBordetellaInfection in Pigs in Northern India

Bordetella bronchisepticaPneumonia Post-Liver Resection in a Patient with Liver Cirrhosis and Cholangiocarcinoma

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