Antimicrobial susceptibility of bovine clinical mastitis pathogens in Japan          and development of a simplified agar disk diffusion method for clinical          practice

Kawai, Kazuhiro; Kurumisawa, Tomomi; Shinozuka, Yasunori; Higuchi, Hidetoshi; Iwano, Hidetomo; Hayashi, Tomohito; Ozawa, Manao; Koike, Ryuji; Uchiyama, Mitsuru

doi:10.1292/jvms.21-0450

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…Additionally, swabs from these samples were cultured on blood agar plates (Eiken Kagaku, Tokyo, Japan) and incubated for 48 hours at 37°C. The bacterial colony obtained was identi ed by its 16S ribosomal RNA gene sequence as previously described (17). The sequence of the 16S 27F forward primer was 5′-AGAGTTTGATCCTGGCTCAG-3′, and the 1492R reverse primer was 5′-GGTTACCTTGTTACGACTT-3′.…”

Section: Animalmentioning

confidence: 99%

Biofilm characterization of Mycoplasma bovis co-cultured with Trueperella pyogenes

Nishi,

Gondaira,

Hirano

et al. 2024

Preprint

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Mycoplasma pneumonia caused by Mycoplasma bovis (M. bovis) is associated with severe inflammatory reactions in the trachea and lungs and can be difficult to treat with antibiotics. Biofilms play a significant role in the persistence of bacteria and contribute to chronic lesions. A recent study showed that polymicrobial interactions of species are an important factor in biofilm formation, but the detailed mechanism of biofilm formation of M. bovis remains unknown. Assuming multiple pathogen infections in bovine respiratory disease complex, this study examined the characterization of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Bacterium-like aggregation structures (> 10 µm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released extracellular matrix to connect with neighboring bacteria and form a mature biofilm on the plate. Biofilm formation in co-culture of M. bovis and T. pyogenes tended to increase compared to that in single culture of these bacteria. Additionally, some large aggregates (> 40 µm) composed of M. bovis and T. pyogenes were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induced biofilm formation, which was associated with increased resistance to antimicrobial agents, thereby exacerbating the progression of chronic Mycoplasma pneumonia.

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Section: Animalmentioning

confidence: 99%

Biofilm characterization of Mycoplasma bovis co-cultured with Trueperella pyogenes

Nishi,

Gondaira,

Hirano

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Antimicrobial susceptibility of bovine clinical mastitis pathogens in Japan and development of a simplified agar disk diffusion method for clinical practice

Abstract: Antimicrobial susceptibility of bovine clinical mastitis pathogens in Japan and development of a simplified agar disk diffusion method for clinical practice Kazuhiro KAWAI †1) 2) , Tomomi KURUMISAWA †1) 2) , Yasunori SHINOZUKA *1) 2) ,

Cited by 1 publication

References 18 publications

Biofilm characterization of Mycoplasma bovis co-cultured with Trueperella pyogenes

Biofilm characterization of Mycoplasma bovis co-cultured with Trueperella pyogenes

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