Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium

Vandenberg, Olivier; Houf, Kurt; Douat, Nicole; Vlaes, L; Retore, Patricia; Butzler, Jean‐Paul; Dediste, Anne

doi:10.1093/jac/dkl080

Cited by 105 publications

(91 citation statements)

References 30 publications

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“…The MICs of antibiotics against the C. upsaliensis strain are shown in Table 1. Antibiotic agents like penicillins, cephalosporins, and macrolides, including AZM, showed low MICs, consistent with previous reports (3)(4)(5). On the other hand, the MIC of fluoroquinolone (levofloxacin) was Ͼ8 g/ml, indicating resistance to fluoroquinolone, as seen in a previous report (4).…”

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confidence: 90%

First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

et al. 2015

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c We encountered a rare case of severe fatal infection in a 70-year-old woman due to Campylobacter upsaliensis, identified by PCR amplification and sequencing analysis of the 16S rRNA gene using DNA extracted from the isolates. To our knowledge, fatal sepsis due to this organism has never been described to date. CASE REPORTA 70-year-old woman was admitted to the emergency department complaining of loss of consciousness. On admission, her vital signs showed lethargy, hypothermia (35.1°C), hypotension (systolic blood pressure of 80 mm Hg), and tachycardia (pulse rate of 160 per minute). She did not have any recent episodes of diarrhea or enterocolitis, as assessed at her periodic visits to our hospital. Prolonged hypoglycemia with treatment resistance was also observed, and glucose was administered more than once. She had a history of left ureteral cancer, thyroid cancer, ectopic Cushing's syndrome, hypertension, and atrial fibrillation. Laboratory examination showed a normal white blood cell count, thrombocytopenia, a high serum transaminase level, and acute kidney injury (estimated glomerular filtration rate of 15.4 ml/min/liter). Severe metabolic acidosis and lactic acidosis were also observed. Enhanced computed tomography imaging led to a diagnosis of Stanford type A dissecting aortic aneurysm with cardiac tamponade. Enteritis and abscesses were not observed. Although pericardiocentesis was performed for the cardiac tamponade caused by the dissecting aortic aneurysm, the patient chose not to have artificial blood vessel replacement performed. Large amounts of vasopressors (noradrenaline and dopamine) and glucose were administered while the patient was in the intensive care unit. Cefazolin (2 g per day) was administered to prevent surgical site infection following pericardiocentesis rather than as a treatment for the original infection. However, severe hypotension, hypoglycemia, and liver failure worsened without improvement. The patient died due to multiorgan failure 2 days after admission.Bacterial isolates from the blood of the patient, which was taken at admission for the evaluation of hypertension, were positive in Bactec aerobic bottles alone (BD, Franklin Lakes, NJ, USA). Subculture of the positive blood culture bottle yielded growth after 72 h of microaerobic incubation at 37°C (2 days after the patient's death). Bacterial identification using API Campy (Sysmex-bioMérieux, Tokyo, Japan) did not yield a definitive identification. Therefore, PCR amplification and sequencing were performed to analyze the 16S rRNA gene using DNA extracted from the isolates. Genomic DNA from all strains grown were extracted by physical extraction using zirconia beads (Mora extraction kit; AMR Co., Gifu, Japan), according to the manufacturer's instructions. The universal primers 8UA (5=-AGAGTTTGATCMTGGCTCAG-3=) and 1485B (5=-ACGGGCGGTGTGTRC-3=) were used as described previously (1). We performed sequencing analysis using a GenBank BLAST search and EzTaxon (http://www.ezbiocloud.net /eztaxon/). The sequence of the 16S rRNA gen...

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First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

et al. 2015

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“…However, 100% isolates were resistant to nalidixic acid, ciprofloxacin and sulphamethoxazole-trimethoprim. Susceptibility tests for campylobacter species are not standardized (there is no recommendation by the CLSI), so there are variabilities in the susceptibility reported (Vandenberg et al, 2006). However, we used disk diffusion method for the susceptibility testing of C. jejuni and C. coli strains used in this study followed by determination of MIC of the intermediately resistant and resistant strains by agar dilution method (Luangtongkum et al, 2007).…”

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confidence: 99%

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Kabir

Asakura

Shiramaru

et al. 2015

Asian J. Med. Biol. Res

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Abstract:The current study aimed to identify the selected number of Campylobacter strains of poultry origin in India that were isolated in the Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan to the species level with the aid of cdtA/B/C gene-based multiplex PCR assays as well as to investigate their antimicrobial resistance profiles. C. jejuni (4 strains) and C. coli (16 strains) were identified. The poultry isolates identified were subjected to susceptibility testing with the aid of disk diffusion method using 12 antimicrobial agents. Again, the resistant and intermediate isolates confirmed by the disk diffusion method were subjected to determination of minimum inhibitory concentration by agar dilution method. To elucidate the mechanism of quinolone resistance, a total number of 20 quinolone resistant strains were subjected to sequence determination and analysis of the gyrA gene in the quinolone-resistance determining region. The results of the disk diffusion method were consistent with the results of the agar dilution method with slight variation in case of ampicillin, levofloxacin, ofloxacin and fosfomycin. All C. jejuni and C. coli strains had the Thr 86 to Ile substitution in gyrA. Results of this study support the emergence of resistance of C. jejuni and C. coli strains of poultry origin in India to a variety of antimicrobials especially fluoroquinolones.

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“…However, susceptibility to oxytetracycline and erythromycin was detected in the same investigation in contrast to our study. In the study of Vandenberg et al (2006), 78.7% of the A. butzleri isolates were found to be susceptible to ampicillin and erythromycin, and all (100%) were susceptible to tetracycline. In the studies of Atabay and Aydin (2001) on samples from broiler chickens, and of Kabeya et al (2004) on beef, pork and chicken meat in Japan, the Arcobacter spp.…”

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confidence: 98%

“…The presence of Arcobacter spp. has been demonstrated in foods of animal origin, various animal faeces (such as cattle, sheep, dog, rabbit, chicken), the environment and water in many studies (Kabeya et al 2003;Van Driessche et al 2003;Vandenberg et al 2006;Houf et al 2008;De Smet et al 2011;Shah et al 2011;Noh et al 2012;Suelam 2012). In their study, Figueras et al (2012) increased the number of currently characterised Arcobacter spp.…”

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Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method

Yesilmen¹,

Vural²,

Erkan³

et al. 2017

Vet. Med.

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ABSTRACT:In this study, the presence of Arcobacter spp. was investigated in the faeces of cattle, sheep, goats, dogs and cloacal swab samples of chickens using the 16S rDNA-RFLP method. The prevalence of Arcobacter in these species was found to be 13%, 12%, 16%, 4% and 33%, respectively. On the other hand, Arcobacter spp. could not be isolated from rabbit faeces. A total of 78 (13%) Arcobacter spp. isolates were obtained from the 500 faecal samples and 100 cloacal swab samples examined in this study. From these 78 Arcobacter isolates, 24 (30.8%), 20 (25.6%), 11 (14.1%), 8 (10.7%), 4 (5.1%), 3 (3.9%) and 2 (2.6%) were identified by 16S rDNA-RLFP as A. cryaerophilus, A. butzleri, A. skirrowii, A. cloacae, A. cibarius, A. halophilus, and A. nitrofigilis, respectively. All A. cryaerophilus (n = 24) isolates were found to be resistant to cloxacillin; all A. butzleri (n = 20) and A. skirrowii isolates were found to be resistant to penicillin/novobiocin, cefoperazone, tetracycline and cloxacillin. It was determined in this study that clinically healthy cattle, sheep, goats, dogs and chickens are reservoirs of Arcobacter spp.

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Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium

Abstract: Fluoroquinolones should be considered in the treatment of severe C. upsaliensis and A. butzleri infection. When clinically indicated, erythromycin remains the first choice for the treatment of intestinal campylobacteriosis caused by C. concisus and C. fetus.

Cited by 105 publications

References 30 publications

First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method

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