Antimicrobial susceptibility of Clostridium perfringens isolated from domestic and wild animal species in Brazil

Abstract

Clostridium perfringens is a microorganism commonly found in the microbiota of humans and animals and a potential cause of enteric, muscle or nervous diseases. The treatment of these diseases is based on antimicrobial therapy and it is extremely important to know the antimicrobial susceptibility profile of the strains present in the region. The aim of this study was to evaluate the antimicrobial susceptibility of C. perfringens isolated from domestic and wild animals in Brazil against seven different antimicrobials. Forty-one strains from the stool samples of cattle (n = 12), buffalo (n = 2), goat (n = 3), dogs (n = 12) and wild carnivores (n = 12) were examined. The minimum inhibitory concentration was determined by the agar dilution method using Brucella agar supplemented with 5% of sheep blood, 0.1% of vitamin K, 0.1% of hemin and concentrations ranging from 0,25 to 256,0 mg L -1 of the following antibiotics: erythromycin, florfenicol, metronidazole, oxytetracycline, penicillin, tylosin, and vancomycin. All C. perfringens strains were susceptible to florfenicol, metronidazole, penicillin and vancomycin. Two strains (4.9%) were resistant to erythromycin and tylosin, while five (12.2%) were resistant to oxytetracycline, one of which (2.4%) from an ocelot.

Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

“…However, it was not possible to determine the antibiotic use in the three farms whose manure was treated by anaerobic digestion. On the whole, the MIC 50 observed in manure and digestates for the antibiotic tested resembled those reported in animal feces [22,27,29,38,49,50], suggesting that storing manure and anaerobic digestion have no impact on the antimicrobial susceptibility of C. perfringens originating from farm animals.…”

Molecular characterization of some pathogenic bacterial strains and hematobiochemical profile in Barki sheep with diarrhea in Siwa Oasis

“…Mansoura Vet Med J 23:4 (2022) [59][60][61][62][63][64][65][66][67][68][69] Oligonucleotide Primers: The primers used were supplied by Metabion (Germany) and are listed in table (2), Cycling conditions used during polymerase chain reaction for detection of bacterial genes listed in table (3).…”