Antimicrobial Susceptibility of <i>Pasteurella multocida</i> Isolated From Cattle and Pigs

Yoshimura, Haruo; Ishimaru, M.; Endoh, Y. S.; Kojima, Akira

doi:10.1111/j.1439-0450.2001.00468.x

Cited by 13 publications

(14 citation statements)

References 12 publications

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“…The antimicrobial susceptibility data from this study indicate that cephalosporins, florfenicol, tetracyclines, and fluoroquinolones are the most effective drugs, a fact also reported in studies conducted in France, North America, and Japan [20–22]. The high resistance of the isolates against sulfonamides and cotrimoxazole has also been previously described [9].…”

Section: Discussionsupporting

confidence: 78%

Virulence Genes and Antimicrobial Resistance Profiles ofPasteurella multocidaStrains Isolated from Rabbits in Brazil

Ferreira

Felizardo

Gobbi

et al. 2012

The Scientific World Journal

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Pasteurella multocida is responsible for a wide range of diseases in domestic animals. In rabbits, the agent is related to nasal discharge, pneumonia, otitis media, pyometra, orchitis, abscess, and septicemia. One hundred and forty rabbits with respiratory diseases from four rabbitries in São Paulo State, Brazil were evaluated for the detection of P. multocida in their nasal cavities. A total of twenty-nine animals were positive to P. multocida isolation, and 46 strains were selected and characterized by means of biochemical tests and PCR. P. multocida strains were tested for capsular type, virulence genes, and resistance profile. A total of 45.6% (21/46) of isolates belonged to capsular type A, and 54.34% (25/46) of the isolates were untypeable. None of the strains harboured toxA or pfhA genes. The frequency of the other twenty genes tested was variable, and the data generated was used to build a dendrogram, showing the relatedness of strains, which were clustered according to origin. Resistance revealed to be more common against sulfonamides and cotrimoxazole, followed by erythromycin, penicillin, and amoxicillin.

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Section: Discussionsupporting

confidence: 78%

Virulence Genes and Antimicrobial Resistance Profiles ofPasteurella multocidaStrains Isolated from Rabbits in Brazil

Ferreira

Felizardo

Gobbi

et al. 2012

The Scientific World Journal

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“…For example, for 50% TAR and a bacteriostatic action, doses were 12 mg/kg (broth) and 232 mg/kg (serum) whilst for 90% TAR and a bactericidal level of kill, corresponding doses were 95 and 1123 mg/kg. Pasteurella multocida MIC distributions for a smaller number of oxytetracycline isolates of Japanese origin (Yoshimura et al, 2001) differed from the tetracycline MIC distributions of European origin obtained by de Jong et al (2014); for example, the MIC 90 s were 12.5 and 2 lg/mL, respectively. Figure 4 demonstrates the MIC distribution data after applying epidemiological cut-off values (ECOFF) to ensure normally distributed data.…”

Section: Dose Determination By Monte Carlo Simulationmentioning

confidence: 62%

“…Pasteurella multocida MIC distributions for a smaller number of oxytetracycline isolates of Japanese origin (Yoshimura et al ., ) differed from the tetracycline MIC distributions of European origin obtained by de Jong et al . (); for example, the MIC 90 s were 12.5 and 2 μ g/mL, respectively.…”

Section: Resultsmentioning

confidence: 97%

“…4). In addition, for comparative purposes, more limited Japanese oxytetracycline MIC distribution data for P. multocida in pigs were used (Yoshimura et al, 2001). Monte Carlo simulations were based on numerical values and incidence of each input variable and predicted: the daily dose at steadystate ( Fig 5); single loading doses for three time periods, 0-24, 0-48 and 0-72 h; and doses for three levels of bacterial kill, as described by Lees et al (2015a).…”

Section: Dose Determinationmentioning

confidence: 99%

“…Predicted daily doses at steady-state based on oxytetracycline MIC distribution data(Yoshimura et al, 2001) and using broth and serum pharmacodynamic data for Pasteurella multocida…”

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confidence: 99%

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Pharmacokinetic/pharmacodynamic integration and modelling of oxytetracycline for the porcine pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida

Dorey

Pelligand

Cheng

et al. 2017

Vet Pharm & Therapeutics

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Pharmacokinetic–pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules of oxytetracycline for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) were determined in broth and porcine serum. PK/PD integration established ratios of average concentration over 48 h (C av0–48 h)/MIC of 5.87 and 0.27 μg/mL (P. multocida) and 0.70 and 0.85 μg/mL (A. pleuropneumoniae) for broth and serum MICs, respectively. PK/PD modelling of in vitro time–kill curves established broth and serum breakpoint values for area under curve (AUC 0–24 h)/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4 log10 reductions in bacterial count. Doses were then predicted for each pathogen, based on Monte Carlo simulations, for: (i) bacteriostatic and bactericidal levels of kill; (ii) 50% and 90% target attainment rates (TAR); and (iii) single dosing and daily dosing at steady‐state. For 90% TAR, predicted daily doses at steady‐state for bactericidal actions were 1123 mg/kg (P. multocida) and 43 mg/kg (A. pleuropneumoniae) based on serum MICs. Lower TARs were predicted from broth MIC data; corresponding dose estimates were 95 mg/kg (P. multocida) and 34 mg/kg (A. pleuropneumoniae).

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Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

Ziagham,

Gharibi,

Mosallanejad

et al. 2024

Veterinary Medicine & Sci

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BackgroundCompanion animals, including dogs and cats, are frequently identified as sources of Pasteurella multocida, a bacterium that can be transmitted to humans and cause infections.ObjectivesThis survey defines the prevalence, antibiotic sensitivity, capsular types, lipopolysaccharide (LPS) types and virulence factors of P. multocida isolated from cats.MethodsA total of 100 specimens from various cat breeds were collected. P. multocida was characterized using both biochemical tests and PCR. Genotypes of isolates were determined using capsular and LPS typing methods. Additionally, virulotyping was performed by detecting the presence of 12 virulence‐associated genes. Disk diffusion was used to determine the antibiotic sensitivity of the isolates.ResultsThe prevalence of P. multocida in cats was 29%. Among the isolates, the majority were capsular type A (96.5%) and type D (3.4%), with a predominant presence of type A. Twenty‐six of the isolates (89.66%) belonged to LPS genotype L6, whereas three isolates (10.3%) belonged to genotype L3. Among the 12 virulence genes examined, sodC, oma87, ptfA, nanB and ompH showed remarkable prevalence (100%). The toxA gene was detected in four isolates (13.8%). Variations were observed in other virulence genes. The nanH gene was present in 93.1% of the isolates, whereas the pfhA gene was detected in 58.6% of the isolates. The exbD‐tonB, hgbB, sodA and hgbA genes showed prevalence rates of 96.5%, 96.5%, 96.5% and 82.8%, respectively. Additionally, particular capsule and LPS types were associated with specific virulence genes. Specifically, the toxA and pfhA genes were found to be more prevalent in isolates with capsular type A and LPS genotype L6. Most isolates were resistant to ampicillin, clindamycin, lincomycin, streptomycin and penicillin.ConclusionsAccording to this epidemiological and molecular data, P. multocida from cats possess several virulence‐associated genes and are resistant to antimicrobial medicines commonly used in humans and animals. Thus, it is crucial to consider the public health concerns of P. multocida in humans.

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Antimicrobial Susceptibility of Pasteurella multocida Isolated From Cattle and Pigs

Cited by 13 publications

References 12 publications

Virulence Genes and Antimicrobial Resistance Profiles ofPasteurella multocidaStrains Isolated from Rabbits in Brazil

Virulence Genes and Antimicrobial Resistance Profiles ofPasteurella multocidaStrains Isolated from Rabbits in Brazil

Pharmacokinetic/pharmacodynamic integration and modelling of oxytetracycline for the porcine pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida

Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

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