Antimicrobial susceptibility of Streptococcus pneumoniae isolates causing LRTI in Najaf, Iraq

Motaweq, Zahraa Yosif; Naher, Habeeb S.

doi:10.1515/environ-2017-0007

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Streptococcus pnumonia showed a high sensitivity of 100% to ciprofloxacin, 50% to amoxicillin, ampicillin, clindamycin, erythromycin and metronidazole, while it had no resistance to azithromycin, chloramphenicol, gentamicin, tetracyclin, and cefixem. These results are in agreement with [28] and [31] which revealed that S.pneumonia was highly resistant to ampicillin, amoxicillin, cefixem, and azithromycin.…”

Section: Resultssupporting

confidence: 93%

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Isolation and identification of some predominant bacteria and assessment of TNF-α level in serum of patients with gingivitis

Jaseem,

Yasin

2023

Tikrit J. Pure Sci.

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This study was conducted to diagnose the aerobic bacterial species causing gingivitis, reveal the antibiotic susceptibility pattern and assess the level of tumor necrosis factor-alpha (TNF-α) in serum of patients with gingivitis. In total, 110 samples (including patient group and control group) were collected during the period from November 2021 until March 2022. Of which, 80 samples were collected by using oral swabs from patients attending the College of Dentistry at Tikrit University and outpatient clinics of both gender with different age groups. While the remaining 30 samples were collected from healthy individuals representing the control group. Identification of bacterial isolates was performed depending on micro and macroscopic cultural characteristics and biochemical tests. In addition to assessing the biochemical characteristics of the isolates, a VITKE2 compact system was used to ensure the identification of species level. The TNF-α concentrations in the serum were determined using an enzyme-linked immunosorbent assay known as a sandwich ELISA. Out of 80 samples, 60 (75%) samples showed positive bacterial growth cultures, while 20 (25%) samples showed no bacterial growth. The most common isolated bacteria species was Streptococcus mutans (18%), followed by Streptococcus mitis (13%), Staphylococcus aureus (12%) Streptococcus salivarius, Streptococcus pyogenes (8%) Staphylococcus epidermidis, Enterococcus feacalis, Escherichia coli, Klebsilla pneumoniae (6%), Rothia dentocariosa, Pseudomonas aeruginosa (4%), and Streptococcus pneumonia, Kocuria kristinae, Granulicatella adiacens (3%). The sensitivity of the bacterial isolates under study was tested to 11 antibiotics. Different species of bacteria showed various sensitivity patterns to several kinds of antibiotics. The study recorded a high significant difference (P=0.0007) between the patients (56.54 + 9.32 pg/ml) and the control group (31.88 + 7.44 pg/ml) concerning the level of TNF-α. In conclusion, the predominant bacteria identified from gingivitis patients were S. mutans and S. mitis. In addition, the levels of TNF-α in gingivitis patients were significantly higher than in the control group.

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Section: Resultssupporting

confidence: 93%

“…Granulicatella adiacens isolates were susceptible 100% to ampicillin, amoxicillin, chloramphenicol and tetracycline. This result is near to that of [31].…”

Section: Resultssupporting

confidence: 53%

Isolation and identification of some predominant bacteria and assessment of TNF-α level in serum of patients with gingivitis

Jaseem,

Yasin

2023

Tikrit J. Pure Sci.

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“…Problem of macrolide resistant pneumococcus has also been detected in study conducted by Niederman (2015). 13 Although the resistance pattern of cotrimoxazole, ceftriaxone and penicillin did not match with other studies the resistance to ciprofloxacin 13.48% was quite close to the study conducted by Zahraa et al 14 (2017) in which 16.2% of the strains of S. pneumoniae were resistant to ciprofloxacin.…”

Section: Discussioncontrasting

confidence: 43%

Gram Positive Bacteria causing Lower Respiratory Tract Infections and their Resistance Patterns in Kathmandu University Hospital

Sherchan¹,

Humagain

2020

Nep. Med. Coll. J.

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Respiratory tract infection due to Gram positive bacteria is a common cause of morbidity and mortality worldwide. This study of one year duration was carried out to determine the antibiotic resistance pattern of Gram-positive bacteria from patients with lower respiratory tract infection visiting Kathmandu University Hospital. A total of 1,556 respiratory samples including sputum and endotracheal aspirates were processed following standard guidelines. Antibiotic resistance pattern was determined following Clinical Laboratory Standard Institute guidelines. Gram positive bacteria from total sample were 6.6%. Out of them 86.4% were Streptococcus pneumoniae and 13.6% were Staphylococcus aureus. Majority of S. pneumoniae, 53.9% were resistant to azithromycin, followed by cotrimoxazole 40.4%, ciprofloxacin 13.5% and Ceftriaxone 1.1% but all isolates were sensitive to penicillin. A total of 92.9% S. aureus were methicillin and Ciprofloxacin resistant, 64.3% were resistant to cotrimoxazole and 28.6% resistant to amikacin but all strains were sensitive to linezolid. Majority of multidrug resistant Staphylococcus were detected among patients of age more than 60 years. A total of 77.8% patients had lower respiratory tract infection with acute exacerbation of chronic obstructive pulmonary disease. Majority of diabetic patients had multidrug resistant organism. 70.69% patients of age above 60 had multidrug resistant isolates. Multidrug-resistant Gram positive bacteria were observed in respiratory samples. For effective management of lower respiratory tract infections detailed microbiological diagnosis and susceptibility testing is required.

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“…More S. pneumoniae were isolated from males 15 (60%) than females 10 (40%) This may be due to the fact that smoking is more common in males (12) . Despite variations in percent of isolates, Al-Taaie (13) found that pneumococci had a total incidence among those aged over 49 years.…”

Section: Isolation and Identifi Cation Of Streptococcus Pneumoniaementioning

confidence: 98%

Distribution of Virulence genes in Streptococcus pneumoniae Isolated from Different Baghdad Hospitals

Fahran¹,

Al-Saadi²

2021

Indian Journal of Forensic Medicine & Toxicology

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Streptococcus pneumoniae causes serious infections (pneumonia, meningitis and bacteremia) with significant morbidity and mortality rates. The goal of this study is to isolate and identify S. pneumoniae from clinical samples in Baghdad City hospitals in order to explore the presence of three virulence genes (lytA, ply and psaA) in clinical isolates. A total of 120 separate clinical samples were obtained from inpatients and outpatients with upper and lower respiratory tract infections at different hospitals in Baghdad. Twenty-five isolates of S. pneumoniae were identified by using cultural, morphological and biochemical characteristics as well as the diagnosis by VITEK2 system. Clinical samples were including sputum 19 isolates (76%) and urine 6 isolates (24%). The results of collected specimens showed that male's percentage is highest than female's patients, which was out of 25 isolates are 15 (60%) males and 10 (40%) females, The results also showed that it is much more common in elderly people than in younger people. The genomic DNA of bacterial isolates was applied in a polymerase chain reaction (PCR) to amplify certain genes. The PCR results were: Six isolates (24%) show positive results for the presence of lytA gene. Five isolates (20%) showed positive results for the presence of ply gene. Twenty-three isolates (92%) show positive results for the presence of psaA gene. As concluded from the present study, that the results exhibited that the presence of the virulence genes can be considered as a serious issue.

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Antimicrobial susceptibility of Streptococcus pneumoniae isolates causing LRTI in Najaf, Iraq

Cited by 5 publications

References 13 publications

Isolation and identification of some predominant bacteria and assessment of TNF-α level in serum of patients with gingivitis

Isolation and identification of some predominant bacteria and assessment of TNF-α level in serum of patients with gingivitis

Gram Positive Bacteria causing Lower Respiratory Tract Infections and their Resistance Patterns in Kathmandu University Hospital

Distribution of Virulence genes in Streptococcus pneumoniae Isolated from Different Baghdad Hospitals

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