Antimicrobial susceptibility of Vibrio alginolyticus isolated from oyster in Korea

Abstract

Pathogenic Vibrio alginolyticus, a cause of severe infection in shellfish, as well as in humans, has been found at high frequency around all coastal areas of Korea. The aim of this study was to determine the occurrence of V. alginolyticus, to identify the strains isolated from oysters in West Sea, and to investigate their antimicrobial resistance profiles. Biochemical analyses of the 90 initially recovered presumptive V. alginolyticus colonies indicated that 16 isolates were V. alginolyticus. PCR analysis to detect the presence of the gyrB gene confirmed that 15 (93.8 %) of the 16 isolates were V. alginolyticus. These 15 isolates had the following profiles of resistance against 16 antibiotics: all isolates were resistant to ampicillin and vancomycin, and 26.7 % of the isolates exhibited resistance to cephalothin. A large number of isolates showed intermediate resistance to erythromycin (100 %) and rifampin (73.3 %). Five (33.3 %) of the V. alginolyticus isolates demonstrated multiple resistance to at least three antimicrobials.

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

“…(Odeyemi, ). Kang, Shin, Jang, Jung, and So () reported oyster‐borne V. alginolyticus in Korea showing 100% resistance to ampicillin and vancomycin, and 26.7% resistance to cephalothin. Also, intermediate resistance to erythromycin (100%) and rifampin (73.3%) was observed which was parallel to this study.…”

“…(Odeyemi, 2016). Kang, Shin, Jang, Jung, and So (2016) which was parallel to this study. However, another study has documented the V. parahaemolyticus from scallops exhibiting resistance to ampicillin and streptomycin (Lopatek et al, 2015).…”

NaCl promotes antibiotic resistance by reducing redox states in Vibrio alginolyticus

“…Antibiotic-resistant pathogenic V. alginolyticus, a cause of severe infection in fish and shellfish as well as humans, has been found at high frequency around coastal areas (Kang et al, 2016;Lajnef et al, 2012). An understanding of antibiotic-resistant mechanisms is necessary to control these drug-resistant pathogens (Lin et al, 2015).…”

“…Development of antibiotic resistance by Vibrio species represents a severe threat to both fish farming and human health, since pathogenic Vibrio species are not only a cause of severe infections in aquaculture but also a causative agent for superficial wound or external ear infections, as well as serious systemic infection in humans (Bier et al, 2015;Fernandez-Delgado et al, 2017;Jacobs et al, 2017). Recently, antibiotic-resistant Vibrio species, such as Vibrio alginolyticus, have been frequently isolated and are insensitive to existing antibiotics (Zavala-Norzagaray et al, 2015;Kang et al, 2016). Therefore, an understanding of their antibiotic resistance mechanisms is especially important to control these bacterial pathogens.…”

Detection of Diverse N-Acyl-Homoserine Lactones in Vibrio alginolyticus and Regulation of Biofilm Formation by N-(3-Oxodecanoyl) Homoserine Lactone In vitro

“…It also has a wide geographic distribution, with recorded presence in several marine environments, such as east (He et al, 2016) and south China Sea (Wu et al, 2016), west Korea sea (Kang et al, 2016), and Indian Ocean (Gauzere et al, 2016). This environmental opportunistic pathogen has long been a threat to fishing industry, and has been reported to cause human diseases worldwide.…”