Antimicrobial susceptibility pattern of aerobes in diabetic foot ulcers in a South-Indian tertiary care hospital

Sekhar, M. Sonal; Unnikrishnan, M.K.; Rodrigues, Gabriel; Vyas, Navya; Mukhopadhyay, Chiranjay

doi:10.1016/j.foot.2018.07.002

Cited by 18 publications

(33 citation statements)

References 30 publications

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“…The search in the databases resulted in 507 studies; and, after sorting by title, abstract, and application of the inclusion and exclusion criteria, 43 articles were selected. Then, all of them were submitted to full and critical reading, culminating in the selection of 28 studies of which 16 (57.1%) were found in the Medline database (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) ; 6 (21.4%), in the BVS database (25)(26)(27)(28)(29)(30) , of which 66.7% (n = 4) in the LILACS database (26)(27)(28)30) and 33.3% (n = 2) in the IBECS databases (25,29) ; and 6 articles (21.4%) found at CINAHL (31)(32)(33)(34)(35)(36) .…”

Section: Resultsmentioning

confidence: 99%

“…As for the year of publication, 7.1% matched 2009 (9,25) ; 10.7%, 2010 (10)(11)31) ; 3.6%, 2012 (26) ; 3.6%, 2013 (12) ; 7.1%, 2014 (13,27) ; 10.7%, 2015 (14,(28)(29) ; 3.6%, 2016 (15) ; 25%, 2017 (16)(17)(18)(19)(20)30,32) ; 25% , 2018 (21)(22)(23)(24)(33)(34)(35) ; and 3.6%, 2019 (36) . Regarding the country of the study, 21.4% were done in India (12,15,18,(22)(23)(24) ; 21.4%, in Brazil (13,20,(26)(27)(28)30) ; 10.7%, in China (11,21,32) ; 7.1%, in Turkey (16,35) ; 10.7%, in Spain (25,29,36) ; and 7.1%, in Mexico (14,…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the etiology of ulcers with infection, 78.6% were due to diabetes (10)(11)(12)(14)(15)(16)(17)(18)(19)(21)(22)(23)(24)(25)28,(30)(31)(32)(33)(34)(35)(36) ; 21.4%, venous (9,13,20,(26)(27)29) ; and 3.6%, arterial (29) . Regarding the location of the studies, 35.7% were outpatient (13,16,20,23,(26)(27)29,31,33,36) , and 64.2% occurred in hospital un its (9)(10)(11)(12)(14)(15)(17)(18)(19)(21)(22)(24)…”

Section: Resultsmentioning

confidence: 99%

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Microbiological profile of leg ulcer infections: review study

et al. 2021

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Objectives: to analyze the microbiological profile of leg ulcers of patients treated at outpatient clinics and hospitals regarding the type of microorganism, microbiological selection of antibiotics, and techniques for the collection of culture material. Methods: literature review performed on LILACS, IBECS, MEDLINE, and CINAHL databases, resulting in a descriptive analysis of 27 studies. Results: 35.7% of the studies occurred in an outpatient care scenario; and 64.2% in hospitals. There was a predominance of swab (100%) in outpatient care and biopsy (55.5%) in the hospital. Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were more common at both levels of assistance. Methicillin-resistant Staphylococcus aureus was isolated in both. Conclusions: the microbiological profile of infections was similar, with the presence of resistant bacteria in both environments. This fact causes concern and raises the need for research to elucidate it. The studies did not compare the effectiveness between biopsy and swab.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Microbiological profile of leg ulcer infections: review study

et al. 2021

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“…Typically, infections are caused by P. aeruginosa, S. aureus, Escherichia coli, among others [21]. However, diabetic foot infections can be caused by Acinetobacter [22,23].…”

Section: Discussionmentioning

confidence: 99%

A Study on Acinetobacter baumannii and Staphylococcus aureus Strains Recovered from the Same Infection Site of a Diabetic Patient

et al. 2019

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Diabetic foot ulcer infections are frequently polymicrobial in nature and exhibit increased morbidity and mortality, as well as, treatment failures. Interactions between Acinetobacter baumannii and Staphylococcus aureus were studied, which showed strain-dependent changes in growth and antibiotic susceptibility. This study examined the interactions between two clinical strains of A. baumannii (1929) and S. aureus (1928) that were recovered from skin and soft tissues of a diabetic patient. When S. aureus 1928 and A. baumannii 1929 were co-cultured together, there was no significant decrease in growth in either clinical strains, indicating that both strains can coexist in the same site of infection. Additionally, neither strains experienced statistically significant changes in susceptibility. These findings highlight that these two pathogens can be found in the same niche of infection, which may lead to more aggressive outcome of the infection.

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“…Microbiological processing. One of the wound swabs was processed for aerobic bacteria by streaking on MacConkey agar (HiMedia) (34). Individual colonies were processed by biochemical and microbiological techniques (35).…”

Section: Methodsmentioning

confidence: 99%

Microbial Community Distribution and Core Microbiome in Successive Wound Grades of Individuals with Diabetic Foot Ulcers

Jnana

Muthuraman

Varghese

et al. 2020

Appl Environ Microbiol

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Diabetic foot ulcer (DFU) is a major complication of diabetes with high morbidity and mortality rates. The pathogenesis of DFUs is governed by a complex milieu of environmental and host factors. The empirical treatment is initially based on wound severity since culturing and profiling the antibiotic sensitivity of wound-associated microbes is time-consuming. Hence, a thorough and rapid analysis of the microbial landscape is a major requirement toward devising evidence-based interventions. Toward this, 122 wound (100 diabetic and 22 nondiabetic) samples were sampled for their bacterial community structure using both culture-based and next-generation 16S rRNA-based metagenomics approach. Both the approaches showed that the Gram-negative microbes were more abundant in the wound microbiome. The core microbiome consisted of bacterial genera, including Alcaligenes, Pseudomonas, Burkholderia, and Corynebacterium in decreasing order of average relative abundance. Despite the heterogenous nature and extensive sharing of microbes, an inherent community structure was apparent, as revealed by a cluster analysis based on Euclidean distances. Facultative anaerobes (26.5%) were predominant in Wagner grade 5, while strict anaerobes were abundant in Wagner grade 1 (26%). A nonmetric dimensional scaling analysis could not clearly discriminate samples based on HbA1c levels. Sequencing approach revealed the presence of major culturable species even in samples with no bacterial growth in culture-based approach. Our study indicates that (i) the composition of core microbial community varies with wound severity, (ii) polymicrobial species distribution is individual specific, and (iii) antibiotic susceptibility varies with individuals. Our study suggests the need to evolve better-personalized care for better wound management therapies. IMPORTANCE Chronic nonhealing diabetic foot ulcers (DFUs) are a serious complication of diabetes and are further exacerbated by bacterial colonization. The microbial burden in the wound of each individual displays diverse morphological and physiological characteristics with unique patterns of host-pathogen interactions, antibiotic resistance, and virulence. Treatment involves empirical decisions until definitive results on the causative wound pathogens and their antibiotic susceptibility profiles are available. Hence, there is a need for rapid and accurate detection of these polymicrobial communities for effective wound management. Deciphering microbial communities will aid clinicians to tailor their treatment specifically to the microbes prevalent in the DFU at the time of assessment. This may reduce DFUs associated morbidity and mortality while impeding the rise of multidrug-resistant microbes.

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Antimicrobial susceptibility pattern of aerobes in diabetic foot ulcers in a South-Indian tertiary care hospital

Cited by 18 publications

References 30 publications

Microbiological profile of leg ulcer infections: review study

Microbiological profile of leg ulcer infections: review study

A Study on Acinetobacter baumannii and Staphylococcus aureus Strains Recovered from the Same Infection Site of a Diabetic Patient

Microbial Community Distribution and Core Microbiome in Successive Wound Grades of Individuals with Diabetic Foot Ulcers

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