Antimicrobial Susceptibility Profiling of Staphylococcus aureus Isolates from Milk

Chaalal, Wafaa; Aggad, Hebib; Zidane, K; Saidi, Navid; Kihal, Mebrouk

doi:10.9734/bmrj/2016/24064

Cited by 11 publications

(12 citation statements)

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“…On our study, S. aureus was detected in 37.32% of the isolates. The presence of S. aureus in bulk milk tank in the current study was reported to be higher by 75%, 70.4% 55.7%, 55.26%, and 39.8%, in comparison to previous study from Norway, Brazil, Czech Republic, Algeria, and Kosova, respectively (Jørgensen et al, 2005;Rall et al, 2008;Zouharova & Rysanek, 2008;Chaalal et al, 2016;Mehmeti et al, 2017). By contrast, researchers from Switzerland, Oran, and Brazil reported lower levels of S. aureus than the current study (Muehlherr et al, 2003;Fagundes et al, 2010;Lee et al, 2012;Jamali et al, 2015).…”

Section: Discussioncontrasting

confidence: 90%

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

et al. 2020

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Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. Dne hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. On the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in 40 (75.4%) of the 53 S. aureus isolates. As a result of the study, a high incidence of MRSA was detected in bulk tank milk samples. Antibiotic-resistant bacteria are at risk of being transferred to humans via milk. For safe and healthy milk consumption, uncontrolled use of antibiotics in dairy cows should be avoided.

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Section: Discussioncontrasting

confidence: 90%

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

et al. 2020

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“…Multidrug resistance is defined as resistance of an isolate to two or more antibiotics (Olayinka et al, 2004). This finding is in consonance with the findings of Umaru et al (2013), Anueyiagu and Isiyaku (2015), Tessema (2016), andChaalal et al (2016) who reported cases of multidrug resistance among S. aureus isolated from dairy products in Zaria, Jos, Ethiopia, and Algeria respectively. The isolates were resistant to a combination of three, four, five, and six of the antibiotics tested.…”

Section: Antibioticssupporting

confidence: 77%

“…The percentage occurrence of S. aureus in fresh and fermented milk in parts of Nasarawa State, recorded in this study was 5.0%, which was lower than the 12.6 and 12.14% recorded by Umaru et al (2013) and Usman and Mustapha (2016), in studies conducted to determine the occurrence of S. aureus in fresh and fermented milk in Kaduna and Zaria, respectively. It was also lower than the 8.7% prevalence recorded by Okpo et al 2016 2015, and Chaalal et al (2016). Higher occurrence of the organism in dairy products in Kenya, Turkey, Bangladesh, and Algeria respectively.…”

Section: Statistical Analysesmentioning

confidence: 57%

Antibiotic resistant phenotypes of Staphylococcus aureus isolated from fresh and fermented milk in parts of Nasarawa State, Nigeria

Aliyu¹,

Abdullahi²,

Whong³

et al. 2019

Afr. J. Microbiol. Res.

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This work was aimed at determining the occurrence and antibiogram of Staphylococcus aureus isolated from fresh and fermented milk samples in parts of Nasarawa State, Nigeria. A total of 180 samples comprising of fresh raw milk, bulk milk, nono, and kindirmo were collected over a period of 6 months (May to October, 2017). Standard microbiological procedures were employed in the isolation, identification, characterisation, and determination of the antibiogram of S. aureus from the milk samples. Characterisation of the S. aureus isolates was by morphological, biochemical characteristics using conventional methods, Microgen® STAPH-ID kits. Confirmed isolates were tested for susceptibility or resistance to a panel of 11 commonly used antibiotics using the agar disc diffusion technique. Out of the 180 milk samples examined, 9 S. aureus were isolated giving a prevalence of 5.0%. The occurrence of S. aureus was higher in nono (12.1%) and kindirmo (10.6%) than in fresh raw milk (5.9%). The high occurrence of S. aureus in nono disproved the assertion that fermented foods are not good media for the survival and growth of S. aureus. The antibiotic susceptibility profile of the S. aureus isolates indicated all of the nine isolates were completely resistant to cefoxitin, ampicillin, and amoxicillin/clavulanic acid. The isolates were moderately resistant to erythromycin (22.2%), sulphamethoxazole/trimethoprim (22.2%), and tetracycline (44.4%). Five antibiotic resistance patterns were recorded among the isolates. All of the isolates had a multiple antibiotics resistance (MAR) index of 0.3 and above, an indication of possible antibiotic misuse in the areas studied.

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“…In all clinical isolates, the resistance rate of S. aureus to gentamicin was 7.7% which was similar (7.0%) with a study performed in Algeria [ 25 ]. In Algeria, no resistance to gentamicin was found in staphylococci in milk [ 54 , 55 ]. One S. aureus isolate exhibiting phenotypic resistance to gentamicin harboured the aac A- aph D gene which is in contrast to previous studies [ 16 , 33 ].…”

Section: Main Textmentioning

confidence: 99%

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

et al. 2018

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ObjectiveThe antimicrobial resistance of staphylococci rose worldwide. In total, 96 Staphylococcus isolates from food and clinical samples were collected from two provinces in Algeria. The antimicrobial susceptibility testing was performed and resistance-associated genes were detected.ResultsFifty-one strains were isolated from food samples and differentiated into 33 Staphylococcus aureus and 18 coagulase-negative staphylococci. Forty-five staphylococci were collected from hospital and community-acquired infection cases. All S. aureus isolated from food were resistant to penicillin and 45.5% were resistant to tetracycline. The resistance rates of 45 clinical Staphylococcus isolates were 86.7%, 48.9%, 37.8% and 20.0% to penicillin, tetracycline, erythromycin and kanamycin, respectively. Nine isolates were confirmed as MRSA from food and clinical isolates. One S. aureus originated from food was confirmed as vancomycin-resistant. Multidrug-resistance was observed among 25.5% and 53.3% of food and clinical staphylococci, respectively. The tetM/K, blaZ, aacA-aphD, ermC and mecA genes were detected in food and clinical isolates. ermA gene was not found. This study provided insight into the status of antimicrobial resistance of staphylococci isolated from food and clinical samples in Algeria. Further investigations and surveillance programmes are mandatory.

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Antimicrobial Susceptibility Profiling of Staphylococcus aureus Isolates from Milk

Cited by 11 publications

References 0 publications

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Antibiotic resistant phenotypes of Staphylococcus aureus isolated from fresh and fermented milk in parts of Nasarawa State, Nigeria

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

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