Antimicrobial susceptibility testing of rapidly growing mycobacteria by microdilution -$$$ Experience of a tertiary care centre

Set, Reena; Rokade, S; Agrawal, Shashank; Shastri, Jayanthi

doi:10.4103/0255-0857.58729

Cited by 11 publications

(8 citation statements)

References 6 publications

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“…With regard to tetracycline, the present study showed tigecycline antimicrobial activity, while minocycline and doxycycline did not, as previously reported by Wallace et al (2002). The isolate was resistant to imipenem which is in accordance with a previous study (Set et al 2010). Among aminoglycosides, amikacin was active, whereas tobramycin showed intermediate activity.…”

Section: Discussionsupporting

confidence: 92%

Nontuberculous mycobacteria in captive and pet reptiles

et al. 2017

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The aim of this study was to highlight the importance of nontuberculous mycobacteria species in the pathology of various reptilian pet species as well as their epidemiological significance of infection transmission to humans. Faeces samples from six living reptiles and organs from ten carcasses were submitted to bacteriological testing during the years 2003-2015. Positive colonies from one faeces sample and two organs showed the presence of a gene coding 65kDa antigen common for all mycobacteria. Further identification to the species level revealed that the isolates belong to Mycobacterium fortuitum and Mycobacterium avium subsp. hominissuis, later subjected to drug susceptibility testing which confirmed high resistance levels in both isolates. In conclusion, there is a great significance of the occurrence of nontuberculous mycobacteria in captive and pet reptiles, presenting reptiles as possible hosts representing a serious threat of transmission of high resistance mycobacterial isolates to humans. To our knowledge, this is the first report of M. avium subsp. hominissuis occurrence in reptiles.

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Section: Discussionsupporting

confidence: 92%

Nontuberculous mycobacteria in captive and pet reptiles

et al. 2017

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“…We have also found M. fortuitum resistant to clarithromycin. [63] Many studies have shown that M. fortuitum contains an inducible erythromycin methylase gene erm which may confer macrolide resistance. Some investigators believe that differences in antibiotic resistance rates in RGM maybe the result of population or geographical differences or may be due to the method of susceptibility testing.…”

Section: Resultsmentioning

confidence: 99%

Laboratory aspects of clinically significant rapidly growing mycobacteria

Set

Shastri

2011

Indian Journal of Medical Microbiology

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The pathogenic potential of the rapidly growing mycobacteria (RGM) has started being recognized. This is due to more sensitive and specific techniques in the laboratory. The RGM are generally defined as nontuberculous species of mycobacteria that show visible growth on agar media within 7 days. RGM are widely distributed in nature and have been isolated from natural water, tap water, and soil. Several biochemical tests, high performance liquid chromatography, and molecular techniques have been developed for rapid identification of these species. The American Thoracic Society and the Infectious Disease Society of America recommend that RGM should be identified to the species level using a recognized acceptable methodology such as polymerase chain reaction restriction enzyme analysis or biochemical testing and routine susceptibility testing of RGM should include amikacin, imipenem, doxycycline, the fluorinated quinolones, a sulphonamide or trimethoprim-sulphamethoxazole, cefoxitin, clarithromycin, linezolid, and tobramycin. The diseases caused by these organisms have varied manifestations. They have been responsible for a number of healthcare-associated outbreaks and pseudo-outbreaks. For recognition of outbreaks, it is important to be familiar with the causative organisms like RGM which are most frequently involved in healthcare-associated outbreaks and pseudo outbreaks. It is essential to intervene as soon as possible to interrupt this transmission. Large gaps still exist in our knowledge of RGM. Unquestionably more studies are required. Through this review, we wish to emphasize that reporting of RGM from clinical settings along with their sensitivity patterns is an absolute need of the hour.

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“…En este estudio, 8 de las 14 cepas (57%) de M. abscessus fueron inhibidas por IMP. Por el contrario, Heidarieh et al hallaron una sensibilidad para este antibiótico menor al 20% en cepas M. abscessus aisladas en varias regiones de Irán (10,(27)(28)(29)(30).…”

Section: Discussionunclassified

Comparação e avaliação de métodos quantitativos para determinar a susceptibilidade antimicrobiana de cepas do complexo Mycobacterium abscessos

Ramírez

Morcillo²,

Imperiale

et al. 2018

Rev. Cienc. salud

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Introducción: el complejo Mycobacterium abscessus incluye especies patógenas emergentes multirresis- tentes, lo cual limita las opciones terapéuticas para tratar las infecciones causadas por dichos microorganismos. En este estudio se compararon las concentraciones inhibitorias mínimas —cim— obtenidas mediante dos métodos cuantitativos, se establecieron los puntos de corte empleados en el micrométodo colorimétrico —mmc— y se evaluó la susceptibilidad antimicrobiana. Materiales y métodos: la cim de nue- ve antibióticos fue determinada mediante el mmc y la microdilución en caldo —mdc— para 19 cepas del complejo M. abscessus. El test F de Snedecor se utilizó para establecer la diferencia signi cativa de las cim entre los dos métodos y se determinaron los puntos de corte mediante la técnica de distribución de la probabilidad para el mmc. Resultados y discusión: se encontró una correlación de los resultados de la cim del 50% entre mmc y mdc para los antibióticos ensayados. Probablemente esta discrepancia en los resulta- dos se deba a diferencias en algunos parámetros técnicos de cada procedimiento. Todas las cepas fueron sensibles a la amikacina y resistentes a meropenem y ampicilina-sulbactam. Independientemente de la especie del complejo M. abscessus, las uoroquinolonas mostraron una baja actividad inhibitoria (0-25%) sobre los aislados clínicos, resultados que son similares a los reportados por otros autores. Los patrones de multirresistencia observados en las cepas analizadas sugieren la necesidad de utilizar las pruebas de susceptibilidad como herramientas que permitan orientar y optimizar las conductas terapéuticas en infecciones producidas por M. abscessus.

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Antimicrobial susceptibility testing of rapidly growing mycobacteria by microdilution -$$$ Experience of a tertiary care centre

Abstract: We wish to emphasize that reporting of rapidly growing mycobacteria from clinical settings, along with their sensitivity patterns, is an absolute need of the hour.

Cited by 11 publications

References 6 publications

Nontuberculous mycobacteria in captive and pet reptiles

Nontuberculous mycobacteria in captive and pet reptiles

Laboratory aspects of clinically significant rapidly growing mycobacteria

Comparação e avaliação de métodos quantitativos para determinar a susceptibilidade antimicrobiana de cepas do complexo Mycobacterium abscessos

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