Antimicrobial Weapons of Pseudomonas aeruginosa

Nolan, Laura M.; Allsopp, Luke P.

doi:10.1007/978-3-031-08491-1_8

Cited by 8 publications

(4 citation statements)

References 260 publications

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“…Yet, due to their replicative nature, phages are difficult to validate pharmacokinetically, as well as to set up administration schemes, and they are used mainly for compassionate treatments [42]. Interestingly, bacteria can also produce phage-like particles that they use to kill competing species [43]. These are called Tailocins and resemble head-less phage particles that kill by puncturing the bacteria membrane, resulting in death by dissipation of the proton-motive force and cellular collapse [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Engineering of Salmonella Phages into Novel Antimicrobial Tailocins

Woudstra,

Sørensen,

Brøndsted

2023

Cells

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Due to the extensive use of antibiotics, the increase of infections caused by antibiotic-resistant bacteria is now a global health concern. Phages have proven useful for treating bacterial infections and represent a promising alternative or complement to antibiotic treatment. Yet, other alternatives exist, such as bacteria-produced non-replicative protein complexes that can kill their targeted bacteria by puncturing their membrane (Tailocins). To expand the repertoire of Tailocins available, we suggest a new approach that transforms phages into Tailocins. Here, we genetically engineered the virulent Ackermannviridae phage S117, as well as temperate phages Fels-1, -2 and Gifsy-1 and -2, targeting the food pathogen Salmonella, by deleting the portal vertex or major capsid gene using CRISPR-Cas9. We report the production of Tailocin particles from engineered virulent and temperate phages able to kill their native host. Our work represents a steppingstone that taps into the huge diversity of phages and transforms them into versatile puncturing new antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Engineering of Salmonella Phages into Novel Antimicrobial Tailocins

Woudstra,

Sørensen,

Brøndsted

2023

Cells

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“…Yet, due to their replicative nature, phages are difficult to validate pharmokinetically as well as setting up administrative schemes and are used mainly for compassionate treatments (42). Interestingly, bacteria can also produce phage-like particles that they use to kill competing species (43). These are called Tailocins and resemble head-less phage particles (44).…”

Section: Discussionmentioning

confidence: 99%

Engineering ofSalmonellaphages into novel antimicrobial Tailocins

Woudstra,

Sørensen,

Brøndsted

2023

Preprint

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Due to the extensive use of antibiotics, the increase of infections caused by antibiotic resistant bacteria are now a global health concern. Phages have proven useful for treating bacterial infections and represent a promising alternative or complement to antibiotic treatment. Yet, other alternative exists, such as bacteria-produced non-replicative protein complexes that can kill their targeted bacteria by puncturing their membrane (Tailocins). To expand the repertoire of Tailocins available, we suggest a new approach transforming phages into Tailocins. Here we genetically engineered the virulentAckermannviridaephage S117, as well as temperate phages Fels-1, -2 and Gifsy-1 and -2 targeting the food pathogenSalmonella, by deleting theportal vertexormajor capsidgene using CRISPR-Cas9. We report the production of Tailocin particles from engineered virulent and temperate phages able to kill their native host. Our work represents a steppingstone to tape into the huge diversity of phages and transform them into versatile puncturing new antimicrobials.

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“…Some are phospholipases that alter the composition of lipid bilayers and cause cell lysis. Contact-independent killing typically involves other secretion systems that secret soluble factors such as bacteriocins and other small antimicrobial molecules that can diffuse through the environment and affect distant cells [ 98 , 99 , 100 ]. Rhs from Dickeya dadantii is an effector secreted by T6SS, which has a conserved central region and variable N- and C-terminal regions [ 101 ].…”

Section: The Love and Hate Relationshipmentioning

confidence: 99%

The Love and Hate Relationship between T5SS and Other Secretion Systems in Bacteria

Luo,

Chen,

Lian

et al. 2023

IJMS

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Bacteria have existed on Earth for billions of years, exhibiting ubiquity and involvement in various biological activities. To ensure survival, bacteria usually release and secrete effector proteins to acquire nutrients and compete with other microorganisms for living space during long-term evolution. Consequently, bacteria have developed a range of secretion systems, which are complex macromolecular transport machines responsible for transporting proteins across the bacterial cell membranes. Among them, one particular secretion system that stands out from the rest is the type V secretion system (T5SS), known as the “autotransporter”. Bacterial activities mediated by T5SS include adherence to host cells or the extracellular matrix, invasion of host cells, immune evasion and serum resistance, contact-dependent growth inhibition, cytotoxicity, intracellular flow, protease activity, autoaggregation, and biofilm formation. In a bacterial body, it is not enough to rely on T5SS alone; in most cases, T5SS cooperates with other secretion systems to carry out bacterial life activities, but regardless of how good the relationship is, there is friction between the secretion systems. T5SS and T1SS/T2SS/T3SS/T6SS all play a synergistic role in the pathogenic processes of bacteria, such as nutrient acquisition, pathogenicity enhancement, and immune modulation, but T5SS indirectly inhibits the function of T4SS. This could be considered a love–hate relationship between secretion systems. This paper uses the systematic literature review methodology to review 117 journal articles published within the period from 1995 to 2024, which are all available from the PubMed, Web of Science, and Scopus databases and aim to elucidate the link between T5SS and other secretion systems, providing clues for future prevention and control of bacterial diseases.

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Antimicrobial Weapons of Pseudomonas aeruginosa

Cited by 8 publications

References 260 publications

Engineering of Salmonella Phages into Novel Antimicrobial Tailocins

Engineering of Salmonella Phages into Novel Antimicrobial Tailocins

Engineering ofSalmonellaphages into novel antimicrobial Tailocins

The Love and Hate Relationship between T5SS and Other Secretion Systems in Bacteria

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