Antimitotic Activity on Sea Urchin Embryonic Cells of Seven Antiparasitic Morita-Baylis-Hillman Adducts: A Potential New Class of Anticancer Drugs

Leite, Jocelmo C. A.; Claudio, G. L.; Silva, Fábio P.L.; Sousa, Suervy C.O.; Vasconcellos, Mário L. A. A.; Marques‐Santos, Luis Fernando

doi:10.2174/157340612804075070

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These compounds might reveal interesting biotechnological applications even in the field of human medicine (Thompson, 1995) since they might have antitumor activity against some cell lines. Indeed, sea urchin embryos have been successfully used to test the antimitotic activity of candidate compounds for chemotherapy, in oncological research (Leite et al, 2012), and the induction of blockage at the first mitotic division might suggest a similar antimitotic effect on fast dividing tumour cells (Gutierrez, 2016). Different tissues demonstrated variable responses to microcystins (Yi et al, 2011) and a recent study (Zhang et al, 2011) demonstrated that the expression level of p53 increased when human Sertoli cells were exposed to microcystins, suggesting that they induce apoptosis by modulating the expression of p53, as well as modulating the expression of Bcl-2 proteins.…”

Section: Discussionmentioning

confidence: 99%

Growth and toxicity of Halomicronema metazoicum (Cyanoprokaryota, Cyanophyta) at different conditions of light, salinity and temperature

et al. 2019

View full text Add to dashboard Cite

Cyanobacteria may live in the water column and in the benthos of aquatic environments, or be symbionts of other organisms, as in the case of Phormidium-like cyanobacteria, known to influence the ecology of freshwater and marine ecosystems. A strain of Phormidium-like cyanobacteria has been recently isolated as a free-living epiphyte of leaves of Posidonia oceanica (L.) Delile in the Mediterranean sea and its biology and ecology are herein investigated. It was identified as Halomicronema metazoicum, previously known uniquely as a symbiont of marine sponges. We cultivated it in a range of light irradiances, temperatures and salinities, to establish the most suitable conditions for the production of allelopathic and toxic compounds. The bioactivity of its spent culture medium was measured by means of standard toxicity tests performed on two model organisms. Our results indicate that at least two bioactive compounds are produced, at low and high irradiance levels and at two temperatures. The main compounds influencing the survival of model organisms are produced at the highest temperature and high or intermediate irradiance levels. The present research contributes to the understanding of critical toxigenic relationships among cyanobacteria and invertebrates, possibly influencing the ecology of such a complex environment as P. oceanica. Future isolation, identification and production of bioactive compounds will permit their exploitation for biotechnologies in the field of ecological conservation and medical applications.

show abstract