Antimutagenic activity of wheat β-purothionin Tk-AMP-BP

Одинцова, Т. И.; Vassilieva, Ioulia; Korostyleva, Tatyana V.; Utkina, L. L.; Slavokhotova, Anna A.; Рогожин, Е. А.; Shiyan, A. N.; Pukhal’skii, V. A.; Zasukhina, G. D.

doi:10.1134/s102279541108014x

Cited by 8 publications

(5 citation statements)

References 15 publications

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“…Apparently, the increase in the transcriptional activity of р53 can explain the increased tolerance of cells treated with these mutagens to various mutagenic effects, which has been repeatedly shown by us [5,6]. The antimutagens had no effect on the activity of the transcription factor NFkB and its target gene IAP 1 both in RD cells and in blood cells.…”

Section: Comparative Analysis Of Gene Expression In Human Blood Cellsmentioning

confidence: 77%

“…In this regard, it is promising to study the effect of antimutagenic agents on the expression of genes that ensure cellular homeo stasis, whose disturbances lead to the development of various pathologies. For this reason, in this work we studied the natural antimicrobial peptide extracted from wheat seedlings, which consists of 45 amino acid residues and was found earlier to exhibit antimu tagenic properties [5]. For comparison, we have cho sen the crown compound (С 18 H 26 N 2 O 7 ), whose anti mutagenic properties we have also described earlier [6] and which is discussed in the literature as a promising anticancer drug [7].…”

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confidence: 99%

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Comparative analysis of gene expression in human blood cells and in rhabdomyosarcoma cells pretreated with antimutagens

Zasukhina

Шишкіна

Vasilyeva

et al. 2014

Dokl Biochem Biophys

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Section: Comparative Analysis Of Gene Expression In Human Blood Cellsmentioning

confidence: 77%

mentioning

confidence: 99%

Comparative analysis of gene expression in human blood cells and in rhabdomyosarcoma cells pretreated with antimutagens

Zasukhina

Шишкіна

Vasilyeva

et al. 2014

Dokl Biochem Biophys

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“…An interesting peculiarity of thionins is a higher total positive charge at neutral pH as well as significant membrane-active features that can impart cytotoxic effects toward some tumor cell lines in vitro. Their influence on the expression level of oncogenes, tumor suppressor genes [ 58 , 59 , 60 ], and ability to bind DNA can also decrease the toxic effect of heavy metal ions [ 61 ]. These properties are also found in thionins from wild dicots [ 62 , 63 , 64 , 65 , 66 ].…”

Section: Investigation Of Structural Determinants Of Other Amps (Tmentioning

confidence: 99%

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Рогожин

Ryazantsev

Smirnov

et al. 2018

Plants

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Cereal-derived bioactive peptides with antimicrobial activity have been poorly explored compared to those from dicotyledonous plants. Furthermore, there are a few reports addressing the structural differences between antimicrobial peptides (AMPs) from cultivated and wild cereals, which may shed light on significant varieties in the range and level of their antimicrobial activity. We performed a primary structure analysis of some antimicrobial peptides from wild and cultivated cereals to find out the features that are associated with the much higher antimicrobial resistance characteristic of wild plants. In this review, we identified and analyzed the main parameters determining significant antifungal activity. They relate to a high variability level in the sequences of C-terminal fragments and a high content of hydrophobic amino acid residues in the biologically active defensins in wild cereals, in contrast to AMPs from cultivated forms that usually exhibit weak, if any, activity. We analyzed the similarity of various physicochemical parameters between thionins and defensins. The presence of a high divergence on a fixed part of any polypeptide that is close to defensins could be a determining factor. For all of the currently known hevein-like peptides of cereals, we can say that the determining factor in this regard is the structure of the chitin-binding domain, and in particular, amino acid residues that are not directly involved in intermolecular interaction with chitin. The analysis of amino acid sequences of alpha-hairpinins (hairpin-like peptides) demonstrated much higher antifungal activity and more specificity of the peptides from wild cereals compared with those from wheat and corn, which may be associated with the presence of a mini cluster of positively charged amino acid residues. In addition, at least one hydrophobic residue may be responsible for binding to the components of fungal cell membranes.

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“…Rogozhin from black cumin (Nigella sativa) seeds, and the crown compound in exposure of human cells to ionizing radiation [58]. Previously, we detected antimutagenic properties of β-purothionin [59] and the effect of this drug on the expression of genes involved in the tumorigenesis process. It was shown that the antimutagens (thionines from wheat germ and cumin seeds and the crown compound) that we investigated in cells of healthy donors reduced expression of genes involved in carcinogenesis, while these drugs had almost no effect on normal cells.…”

Section: Approach To Reducing the Damaging Effects Of Radiation In Humentioning

confidence: 99%

The miRNA as human cell gene activity regulator after ionizing radiation

et al. 2017

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Abstract⎯This review presents analysis of the literature and our own research with respect to the role of miRNAs in the regulation of activity (expression) of genes controlling cellular homeostasis in human cells when exposed to ionizing radiation. Human cells, on one hand, can have increased resistance to radiation, which hinders the effectiveness of tumor treatment in radiotherapy. On the other hand, increased sensitivity to radiation may be accompanied by the development of several pathologies, including tumorigenesis. This paper examines the role of specific miRNAs in the formation of radioresistance and radiosensitivity of human cells and their impact on the respective target genes. Separate sections are devoted to the role of different miRNAs in radiation therapy of tumors of different localization, as well as their role in the bystander effect. A special section highlights features of gene activity and its regulators, miRNAs, in radiosensitive cells in patients with Down syndrome. The final section provides information about new approaches to change miRNA expression and, accordingly, their target genes by the action of plant and synthetic drugs (crown compounds) which reduce damaging effects of mutagens. It is assumed that antimutagens affecting the expression levels of miRNAs and structural genes may be used to correct the increase and decrease in cellular radioresponse, reducing the risk of development of pathological processes, including tumorigenesis.

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Antimutagenic activity of wheat β-purothionin Tk-AMP-BP

Cited by 8 publications

References 15 publications

Comparative analysis of gene expression in human blood cells and in rhabdomyosarcoma cells pretreated with antimutagens

Comparative analysis of gene expression in human blood cells and in rhabdomyosarcoma cells pretreated with antimutagens

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

The miRNA as human cell gene activity regulator after ionizing radiation

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