Antimutagenic and antigenotoxic effects of vegetable matrices on the activity of pesticides

Isidori, Marina; Caterino, Emanuela; Criscuolo, Emma; Fatigati, Veronica; Liguori, Giovanni; Parrella, Alfredo

doi:10.1080/02652030902896194

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…However, some controversial data exist regarding fludioxonil and cyprodinil. The Ames mutagenicity data reported by EFSA for these two fungicides gave negative results, whereas more recently, Isidory and coworkers [Isidori et al, 2009] reported that these compounds were shown to be poten-tially mutagenic with both the Ames assay and the SOS chromotest. Moreover, in vitro chromosomal aberration was observed with fludioxonil on CHO cells as well as unscheduled DNA synthesis in primary rat hepatocytes [EFSA, 2007].…”

Section: Discussionmentioning

confidence: 97%

Genotoxicity of pesticide mixtures present in the diet of the French population

Graillot

Takakura

Hégarat

et al. 2012

Environ and Mol Mutagen

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Consumers may be simultaneously exposed to several pesticide residues in their diet. A previous study identified the seven most common pesticide mixtures to which the French population was exposed through food consumption in 2006. The aim of this study was to investigate if the seven mixtures are potentially cytotoxic and genotoxic and if so, whether compounds in a same mixture have a combined effect. The cytotoxicity and genotoxicity of the seven mixtures were investigated with a new assay (γ-H2AX) using four human cell lines (ACHN, SH-SY5Y, LS-174T, and HepG2). Mixtures were tested at equimolar concentrations and also at concentrations reflecting their actual proportion in the diet. Irrespective of the cell line tested, parallel cytotoxicity of the seven mixtures was observed. Only one mixture was genotoxic for the HepG2 cells at concentrations = 3 μM in equimolar proportion and at 30 μM in actual proportion. Caspase 3/7 activity, the comet assay, and reactive oxygen species production were also investigated using the same mixture and HepG2 cells. Our results suggest that pesticide metabolites from the mixture generated by HepG2 cells were responsible for the observed damage to DNA. Among the five compounds in the genotoxic mixture, only fludioxonil and cyprodinil were genotoxic for HepG2 cells alone at concentrations = 4 and 20 μM, respectively. Our data suggest a combined genotoxic effect of the mixture at low concentrations with a significantly higher effect of the mixture of pesticides than would be expected from the response to the individual compounds. Environ. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 97%

Genotoxicity of pesticide mixtures present in the diet of the French population

Graillot

Takakura

Hégarat

et al. 2012

Environ and Mol Mutagen

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“…On the other hand, the populations studied in this work come from a rural area, where a great amount of vegetables and fruit are consumed. Isidori et al (2009) reported the antimutagenic and antigenotoxic effects of vegetables on the activity of several pesticides using the Ames test. Thus, we could hypothesize that substances present in this kind of diet might have exerted a protective effect against pesticide exposure.…”

Section: Discussionmentioning

confidence: 99%

Biomonitoring of rural workers exposed to a complex mixture of pesticides in the municipalities of Tianguá and Ubajara (Ceará state, Brazil): Genotoxic and cytogenetic studies

Paiva¹,

Cabral²,

Soares³

et al. 2011

Environ and Mol Mutagen

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In recent years, the use of pesticides in agriculture has been steadily increasing, and associations between exposure to agricultural chemicals and DNA damage and cancer have been reported. Brazil is one of the world leaders in pesticide use; however, studies that evaluate the impact of pesticide exposure on cancer incidence and mortality are very scarce in the Brazilian population. The alkaline comet assay and the chromosome aberration (CA) test were used to evaluate primary DNA damage in the peripheral blood lymphocytes of workers exposed to a complex mixture of pesticides in two small rural communities in the municipalities of Tianguá and Ubajara, located in the western part of Ceará State (Northeast Brazil), which are among the largest agricultural areas of the state. The comet assay showed that the damage index and damage frequency observed in the exposed groups were significantly higher in relation to the controls (P < 0.05). On the other hand, no differences were detected regarding structural and numerical CAs in the communities evaluated. Additionally, the observed levels of DNA strand breaks and frequencies of CAs, stratified for exposure time, were not statistically different for individuals of either rural community. Our results suggest that the damages caused by pesticides in our study area were not great enough to induce permanent mutations or to interfere with mitotic apparatus formation; minimal pesticide damages could have undergone cellular repair, explaining the absence of structural and numerical CAs.

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“…In one study, co-incubation of phenolic extract from Fresh bean and aflatoxin B1 was reported to significantly reduce the mutagenicity and thus the possibility of chemical complex formation was proposed as a mechanism [141]. Antimutagenic effects of vegetable matrices on the activity of pesticides were observed in Ames test and SOS Chromotest [144]. In that study, the antimutagenicity of vegetables was proposed to be related to the antioxidant activity.…”

Section: Gautam Et Almentioning

confidence: 98%

Fruits and Vegetables as Dietary Sources of Antimutagens

Gautam¹,

Saxena²,

Kumar³

2016

J Food Chem Nanotechnol

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Mutation is the process leading to heritable changes in the genetic material of an organism and caused mainly by the external factors, including chemical and physical agents, or can also occur spontaneously due to errors in DNA replication, repair, and recombination. Agents contributing to the mutagenic burden in the environment could be from industrial sources, wide spectrum applications of biocides in the agriculture, and other contaminants. As many of these mutagenic chemicals can induce severe disorders in humans including cancer and a large spectrum of inherited diseases, it is important to detect such mutagenic agents precisely and rapidly, and also look for an approach to combat them. Natural occurring dietary antimutagens primarily from health protective foods such as fruits and vegetables could provide a mechanism to counteract the deleterious effect of these mutagens. The World Health Organization (WHO) indicates that one-third of all cancer deaths are preventable and that diet is closely linked to cancer prevention. These health protective phytochemicals particularly antimutagenic ones could provide an effective solution to these concerns. The current review deals with understanding of the mutagenic events, methods of its analysis and a brief compilation of the existing scientific findings related to the dietary sources having potential to counteract the effects of the mutagenic exposures from different sources. The review would provide an opportunity to look into the science, think about the possible future perspectives and mechanism to translate the outcome of the scientific research for benefits of the mankind.

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Antimutagenic and antigenotoxic effects of vegetable matrices on the activity of pesticides

Cited by 5 publications

References 22 publications

Genotoxicity of pesticide mixtures present in the diet of the French population

Genotoxicity of pesticide mixtures present in the diet of the French population

Biomonitoring of rural workers exposed to a complex mixture of pesticides in the municipalities of Tianguá and Ubajara (Ceará state, Brazil): Genotoxic and cytogenetic studies

Fruits and Vegetables as Dietary Sources of Antimutagens

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