Antimutagenic, Cytoprotective and Antioxidant Properties of Ficus deltoidea Aqueous Extract In Vitro

Ooi, Theng Choon; Ibrahim, Farah Wahida; Ahmad, Shakirah; Chan, Kok Meng; Leong, Lek Mun; Mohammad, Nihayah; Siew, Ee Ling; Rajab, Nor Fadilah

doi:10.3390/molecules26113287

Cited by 10 publications

(12 citation statements)

References 32 publications

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“…These findings support the potential use of F. deltoidea for osteoporosis therapy in diabetes [28]. F. deltoidea aqueous extract at all concentrations tested (12.5-100 µg/mL) exhibited antioxidant power, suggesting the cytoprotective effect of F. deltoidea aqueous extract could be partly attributed to its antioxidant properties [27]. The report of T.C.…”

Section: Resultssupporting

confidence: 79%

“…Results showed that pretreatment of FDD (50 and 100 µg/mL) demonstrated remarkable protection against menadione-induced oxidative stress in V79 cells significantly by decreasing superoxide anion level. FDD at all concentrations tested (12.5-100 µg/mL) exhibited antioxidant power, suggesting the cytoprotective effect of FDD could be partly attributed to its antioxidant properties [27].…”

Section: Resultsmentioning

confidence: 92%

“…The report of T.C. Ooi and co-workers [27] highlights that F. deltoidea may provide a chemopreventive effect on mutagenic and oxidative stress inducers. Maizatul H.O.…”

Section: Resultsmentioning

confidence: 98%

“…The antioxidant activities of F. deltoidea aqueous extract (FDD) on menadione-induced oxidative stress were determined in a V79 mouse lung fibroblast cell line by Ooi and coworkers [27]. The ferric-reducing antioxidant power (FRAP) assay was conducted to evaluate FDD antioxidant capacity.…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Tkachenko¹,

Kurhaluk²,

Buyun³

et al. 2023

BHT

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Purpose: to investigate the antioxidant properties of the aqueous extracts derived from leaves of Ficus deltoidea using the model of human blood. For this purpose, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), content of aldehydic and ketonic derivatives of oxidatively modified proteins, total antioxidant capacity (TAC)] in the human erythrocytes after in vitro incubation with aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) were used. Resistance of human erythrocytes after in vitro treatment by aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) was evaluated by HCl-induced hemolysis using a percentage of hemolyzed erythrocytes per each 30 sec. and a total time of hemolysis. Methodology. The leaves of F. deltoidea were collected in M.M. Gryshko National Botanic Garden (Kyiv, Ukraine). Blood (10-20 mL) was obtained from normal volunteers via venipuncture (4 males and 5 females aged 28-53 years old). An erythrocyte suspension at 1% hematocrit was incubated with 4 mM phosphate buffer (pH 7.4) (control) and pre-incubated with the extract of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL, respectively) at 37 °C for 60 min. The level of lipid peroxidation was determined by quantifying the concentration of 2-thiobarbituric acid reacting substances (TBARS). The rate of protein oxidative destruction was estimated from the reaction of the resultant carbonyl derivatives of amino acid reaction with 2,4-dinitrophenylhydrazine (DNFH). The total antioxidant capacity (TAC) in the samples was estimated by measuring the TBARS level after Tween 80 oxidation. The HCl-induced resistance of erythrocytes was measured spectrophotometrically with 0.1M HCl. The significance of differences (significance level, p < 0.05) was examined using the Mann-Whitney U test. Scientific novelty. The treatment of human erythrocytes by extract derived from leaves of F. deltoidea at the final concentration of 5 mg/mL resulted in an increase of TBARS as biomarkers of lipid peroxidation and aldehydic derivatives of oxidatively modified proteins with a simultaneous decrease in the total antioxidant capacity compared to the untreated samples. On the other hand, in vitro treatment of human erythrocytes with an extract derived from leaves of F. deltoidea at the final concentration of 0.5 mg/mL resulted in the same values of TBARS level, aldehydic and ketonic derivatives of OMP, and total antioxidant capacity as the untreated samples. Extract of F. deltoidea at the final concentrations of 5 mg/mL and 0.5 mg/mL possess hemolytic properties to human erythrocyte suspension after 1-h incubation in vitro. Conclusions. The changes in the oxidative stress biomarkers using the in vitro model of human erythrocytes to evaluate the antioxidant activities of the aqueous extract derived from the leaves of F. deltoidea at the two final concentrations (5 mg/mL and 0.5 mg/mL) revealed that high concentration of extract (5 mg/mL) resulted in the increase of lipid peroxidation and protein oxidation with a simultaneous decline in the total antioxidant capacity. HCl-induced hemolysis was activated after the treatment by extract derived from the leaves of F. deltoidea at the two final concentrations. More studies are warranted in the future, to illustrate the potential and mechanisms of F. deltoidea in preventing oxidative stress using different cell models in vitro and different final concentrations of the extract. Also, further studies are warranted to identify the bioactive components that contribute to this protective effect.

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 92%

“…The report of T.C. Ooi and co-workers [27] highlights that F. deltoidea may provide a chemopreventive effect on mutagenic and oxidative stress inducers. Maizatul H.O.…”

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

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In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Tkachenko¹,

Kurhaluk²,

Buyun³

et al. 2023

BHT

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“…The level of reactive oxygen species (ROS), specifically for superoxide anion and hydrogen peroxide were assessed as previously described 63 , 64 . Briefly, the treated LN-18 cells were administered at different time-point intervals before being harvested.…”

Section: Methodsmentioning

confidence: 99%

Curcumin piperidone derivatives induce anti-proliferative and anti-migratory effects in LN-18 human glioblastoma cells

Razali

Lam

Rajab

et al. 2022

Sci Rep

Self Cite

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Curcumin has demonstrated potential cytotoxicity across various cell lines despite its poor bioavailability and rapid metabolism. Therefore, our group have synthesized curcuminoid analogues with piperidone derivatives, FLDP-5 and FLDP-8 to overcome these limitations. In this study, the analogues were assessed on LN-18 human glioblastoma cells in comparison to curcumin. Results from cytotoxicity assessment showed that FLDP-5 and FLDP-8 curcuminoid analogues caused death in LN-18 cells in a concentration-dependent manner after 24-h treatment with much lower IC50 values of 2.5 µM and 4 µM respectively, which were more potent compared to curcumin with IC50 of 31 µM. Moreover, a significant increase (p < 0.05) in the level of superoxide anion and hydrogen peroxide upon 2-h and 6-h treatment confirmed the oxidative stress involvement in the cell death process induced by these analogues. These analogues also showed potent anti-migratory effects through inhibition of LN-18 cells’ migration and invasion. In addition, cell cycle analysis showed that these analogues are capable of inducing significant (p < 0.05) S-phase cell cycle arrest during the 24-h treatment as compared to untreated, which explained the reduced proliferation indicated by MTT assay. In conclusion, these curcuminoid analogues exhibit potent anti-cancer effects with anti-proliferative and anti-migratory properties towards LN-18 cells as compared to curcumin.

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In Vitro Genotoxic and Antigenotoxic Effects of Ten Novel Synthesized 4‐Thiazolidinone Derivatives

Alaylar,

Güllüce,

Turhan

et al. 2023

Chemistry & Biodiversity

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Heterocyclic compounds are found in a variety of drug molecules, and bioactive natural products. 4‐Thiazolidinones (4‐TZDs), which represent an important class of heterocyclic compounds, are of great interest today with their diverse bioactivities. In this study, ten novel 4‐TZD derivatives (C1–C10) were synthesized, characterized by spectroscopic techniques, and their genotoxic, and antigenotoxic properties were investigated in vitro using the Ames Salmonella/microsome mutagenicity assay in the concentration range of 0.2–1.0 mM/plate. The results revealed that none of the compounds were mutagenic on the three different Salmonella typhimurium strains up to the highest concentration tested. Furthermore, in our study, C1, C4, C6, and C9 showed significant, ranging from moderate to strong, antigenotoxic effects against mutagen‐induced DNA damage at relatively higher doses. Among these, C4 had the best potential to inhibit the number of revertant colonies induced by 9‐aminoacridine (9‐AA), with a maximum inhibition rate of 47.9 % for 1.0 mM/plate. As a result, preliminary knowledge about the safety of the use of ten novel synthesized 4‐TZD compounds likely to exhibit many bioactivities was obtained in this study. In addition, the significant in vitro antimutagenic activity of some derivatives increases the importance of studies for the development of new pharmacological agents for cancer prevention.

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Antimutagenic, Cytoprotective and Antioxidant Properties of Ficus deltoidea Aqueous Extract In Vitro

Cited by 10 publications

References 32 publications

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Curcumin piperidone derivatives induce anti-proliferative and anti-migratory effects in LN-18 human glioblastoma cells

In Vitro Genotoxic and Antigenotoxic Effects of Ten Novel Synthesized 4‐Thiazolidinone Derivatives

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