Antimutagenicity Activity of Different Fractions of Zataria multiflora, 
 Achillea wilhelmsii and Camellia sinensis using Ames Test

Dehghan-No, Gholamreza; Sharififar, Fariba; Moshafi, Mohammad Hassan; Behravan, Effat; Dehghan-No, Ali; Rezaei-Gha, Reza

doi:10.3923/jms.2013.459.464

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The plant has anti Herpers simplex, antimutagenicity and antibacterial effects (Mandegary et al, 2013;Sharififaret al, 2007;Arabzadehet al, 2013;Dehghan-Noodeh et al, 2013). This plant contains flavonoids, phenolic acids and terpenoids (Sharififaret al, 2007;Ali et al, 2000;Shaiq et al, 2000).…”

Section: Zataria Multiflora Boiss (Known In Persianmentioning

confidence: 99%

In vitro evaluation of anticholinesterase, antioxidant and antibacterial activity of different fractions of Zataria multiflora boiss

2015

Int. J. Biosci.

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Zataria multiflora has been widely used for health promotion purpose. We have studied anticholinesterase, antioxidant and antibacterial effects of different fractions of a standard extract of Z. multiflora. Rosmarinic acid (RA) content of plant was determined by spectrophotometric method and anticholinesterase and antioxidant activity of various fractions was studied using colorimetric method and diphenylpicrylhydrazyl (DPPH) assay respectively. Minimum inhibitory concentration (MIC) of fractions against a variety of bacteria was determined by agar dilution method. Results: RA content of the plant was 2.2% (w/w). Chloroform fraction (CHF) exhibited the greatest anticholinesterase effect (94.05± 7.6%) followed by petroleum ether fraction (PEF) (88.7±5.1%).Methanolic fraction (MF) exhibited the highest DPPH inhibition (93.3± 4.1%) followed by PEF and CHF (88.7± 3.7% and 85.4± 2.5% respectively). The least MIC was due to PEF and CHF against Gram-negative and Grampositive bacteria respectively. Conclusion: These results indicated that PEF and CHF would be a good candidate for further studies.

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Section: Zataria Multiflora Boiss (Known In Persianmentioning

confidence: 99%

In vitro evaluation of anticholinesterase, antioxidant and antibacterial activity of different fractions of Zataria multiflora boiss

2015

Int. J. Biosci.

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Antimutagenic activity of major fractions of Zataria multiflora Boiss by Ames method

Dehghannoudeh¹,

Moshafi²,

Ohadi³

et al. 2015

Asian J Pharm

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Z ataria multiflora is a medicinal plant that has been interested in antimutagenicity effect because of its high antioxidant activity and richness of flavonoids. Antimutagenicity effect of total extract of the plant has been reported previously. Aerial parts of Z. multiflora were extracted by petroleum ether, chloroform and 80% methanol by liquid-liquid extraction method consequently. The fractions were concentrated in vacuum and dried at 40°C in oven. The genotype of two standard strains of Salmonella typhimurium (TA98, TA100) was confirmed by the evaluation of two important factors of histidine requirement and the presence of R factor. The minimum inhibition concentration (MIC) of the fractions against these two strains was determined by agar dilution method. From each fraction, various concentrations less than MIC were studied for anti-mutagenic test. The sample along with bacterial strain and mutagen agent were incubated at 37°C for 48 h. The number of revertant colonies was counted and compared with control plates. Our results showed that all fractions especially petroleum ether and chloroform ones maintain the number of colonies in the standard range in control plates and prevent from the growth of many strains of bacteria and increase of revertant colonies enhancement in a concentration-dependent manner. This effect was prominent against TA100 starin. Methanolic fraction exhibited anti-mutagen activity just in the highest used concentration in the presence of TA98.

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Antimutagenicity Activity of Different Fractions of Zataria multiflora, Achillea wilhelmsii and Camellia sinensis using Ames Test

Cited by 2 publications

References 11 publications

In vitro evaluation of anticholinesterase, antioxidant and antibacterial activity of different fractions of Zataria multiflora boiss

In vitro evaluation of anticholinesterase, antioxidant and antibacterial activity of different fractions of Zataria multiflora boiss

Antimutagenic activity of major fractions of Zataria multiflora Boiss by Ames method

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