Antineoplastic Effects and Mechanisms of a New RGD Chimeric Peptide from Bullfrog Skin on the Proliferation and Apoptosis of B16F10 Cells

Jiang, Xuan; Zhang, Xin; Fu, Chao; Zhao, Ruili; Jin, Tao; Liu, Mengyue; Pan, Cunhong; Li, Liu An; Ma, Jingyun; Yu, Enyuan; Yu, Xiaoxue; Hu, Ye

doi:10.1007/s10930-021-09980-x

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…We also demonstrated that b-HIV could inhibit PC cell invasion and migration in vitro. Consistent with these observations, a number of studies (17)(18)(19) have found that G0/G1 cell-cycle arrest could prohibit cancer cell migration and invasion. What is more, multiple studies reported by different groups have revealed an association between the G0/G1 phase blockade and anti-PC effects.…”

Section: Discussionsupporting

confidence: 54%

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

et al. 2022

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Pancreatic cancer (PC) is marked with a low survival rate and lack of recognized effective treatment strategy. We investigated the antitumor effect of β-hydroxyisovaleryl-shikonin (β-HIVS) on PC and the associated working mechanism. Cell toxicity was determined using Cell Counting Kit-8 (CCK-8) assay. Acridine Orange/Ethidium Bromide (AO/EB) double-fluorescent staining assay accompanied by flow cytometry was utilized to estimate cell apoptosis. Cell cycle, reactive oxygen species (ROS), and mitochondrial membrane potential were all evaluated using flow cytometry. Transwell and wound healing assays were performed to evaluate cell migration and invasion. Protein expression was analyzed by Western blots. A xenograft mouse model was employed to determine the antitumor effect of β-HIVS in vivo. PC cell viability gradually decreased with increasing β-HIVS while apoptosis was enhanced together with cell-cycle blockage in the G0–G1 phases. β-HIVS induced mitochondrial dysfunction, ROS production, and DNA damage and inhibited the invasive and migratory ability of PC cells. We further confirmed the suppression of EMT and PI3K/AKT pathways as underlying mechanisms. The mouse model treated with the increasing dose of β-HIVS displayed decreased tumor growth rate, along with increased apoptosis. Thus, β-HIVS exerts antitumor effects on PC through inducing apoptosis, ROS production, decreasing mitochondrial membrane potential, and suppressing signal pathways, such as PI3K/AKT. In summary, β-HIVS might be a promising strategy for PC treatment.

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Section: Discussionsupporting

confidence: 54%

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

et al. 2022

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“…Temporin-La is an AMP that kills cancer cells by membrane destabilization and the chimeric peptide showed higher efficacy by altering mitochondrial membrane potential. 53,54 The specificity and efficacy of RGD based ACPs are controlled by the flanking amino acids and cyclised forms of these molecules are often more potent, specific and stable as cyclisation preserves specific RGD conformations. Incorporation of one d -amino acid into a cRGD (cyclic-RGD) induces kinks in the peptide backbone increasing α v β 3 and α v β 1 selectivity.…”

Section: Types Of Acps and Their Mode Of Actionmentioning

confidence: 99%

Peptide therapeutics in the management of metastatic cancers

2022

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“…The peptides, stored in amphibian skin granular glands, can be released in high concentrations into skin secretions when the amphibians are stressed or injured. Peptides, including antimicrobial peptides [ 18 , 19 ], antioxidant peptides [ 20 , 21 ], antiviral peptides [ 22 , 23 ], antitumor peptides [ 24 , 25 ], and other peptides [ 16 ], are extracted, as regulating body internal functions and fertility of traditional Chinese medicine, as well as the ancient Egyptian medicine to cure the pain and diarrhea [ 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Peptides Isolated from Amphibian Skin Secretions with Emphasis on Antimicrobial Peptides

Chen

Liu

Fang

et al. 2022

Toxins

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The skin of amphibians is a tissue with biological functions, such as defense, respiration, and excretion. In recent years, researchers have discovered a large number of peptides in the skin secretions of amphibians, including antimicrobial peptides, antioxidant peptides, bradykinins, insulin-releasing peptides, and other peptides. This review focuses on the origin, primary structure, secondary structure, length, and functions of peptides secreted from amphibians’ skin. We hope that this review will provide further information and promote the further study of amphibian skin secretions, in order to provide reference for expanding the research and application of amphibian bioactive peptides.

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Antineoplastic Effects and Mechanisms of a New RGD Chimeric Peptide from Bullfrog Skin on the Proliferation and Apoptosis of B16F10 Cells

Cited by 4 publications

References 15 publications

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

β-Hydroxyisovaleryl-Shikonin Exerts an Antitumor Effect on Pancreatic Cancer Through the PI3K/AKT Signaling Pathway

Peptide therapeutics in the management of metastatic cancers

Peptides Isolated from Amphibian Skin Secretions with Emphasis on Antimicrobial Peptides

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