Antineutrophil cytoplasmic antibodies in sera from colectomised ulcerative colitis patients and its relation to the presence of pouchitis.

Esteve, María; Mallolas, Judith; Klaassen, Julieta; Abad-Lacruz, A; González-Húix, Ferrán; Cabré, Eduard; Fernández‐Bañares, Fernando; Bertrán, X; Condom, Enric; Martí-Ragué, Joan; Gassull, Miquel A.

doi:10.1136/gut.38.6.894

Cited by 42 publications

(27 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of 250 unique studies identified using the search strategy, 11 studies met the inclusion criteria and were included in the meta‐analysis (eight cohort, three case–control) . There were no randomised controlled trials that addressed this question.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, anti‐ Saccharomyces cerevisiae antibody (ASCA) directed against a cell‐wall component of the yeast, S. cerevisiae , is more specific for CD (seen in 29–69% patients) and uncommon in UC (0–29%). Some studies have shown that the presence of ANCA, especially in high titre, may increase the risk of pouchitis after IPAA, but the results have been conflicting . ASCA‐positivity has been associated with an increased risk of post‐operative fistulae and CD‐like condition of the pouch, but it is unclear whether it influences the risk of pouchitis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Meta‐analysis: serological markers and the risk of acute and chronic pouchitis

Singh

Sharma

Loftus

et al. 2013

Aliment Pharmacol Ther

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Meta‐analysis: serological markers and the risk of acute and chronic pouchitis

Singh

Sharma

Loftus

et al. 2013

Aliment Pharmacol Ther

View full text Add to dashboard Cite

show abstract

“…UC-ANCA have not been shown to impair neutrophil function,7 their presence is not related to the severity or extent of the disease,3 17 18and in a high percentage of patients they may remain for years after colectomy 1718 This evidence argues against a decisive role of UC-ANCA in tissue damage.…”

Section: Discussionmentioning

confidence: 99%

Antineutrophil antibodies associated with ulcerative colitis interact with the antigen(s) during the process of apoptosis

Mallolas

2000

Gut

Self Cite

View full text Add to dashboard Cite

Background-Cell death by apoptosis seems to be an important mechanism for translocation to the cell surface of a variety of intracellular components capable of inducing autoantibody production. Aims-To identify the cellular location of antigen (Ag)-antineutrophil cytoplasmic antibodies (ANCA) in non-apoptotic human neutrophils, and to assess if ANCA associated with ulcerative colitis reacts with neutrophil antigen(s) during neutrophil apoptosis. The cellular distribution of Ag-ANCA in apoptotic neutrophils was also investigated. Methods-Sera from 18 ulcerative colitis patients known to be positive for perinuclear IgG-ANCA (titre >1/320), as assessed by indirect immunofluorescence (IIF), were analysed by immunofluorescent confocal laser scanning microscopy. ANCA were identified with fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) in non-apoptotic and apoptotic neutrophils, respectively. Apoptotic and non-apoptotic DNA was labelled with FITC and propidium iodide, respectively. Cycloheximide was added to polymorphonuclear leucocyte culture to induce apoptosis. Results-Three patterns of scanning laser immunofluorescence microscopy in nonapoptotic neutrophils were observed with respect to cellular ulcerative colitis associated ANCA distribution: (1) diVuse nuclear localisation (16.7%); (2) nuclear localisation in the nuclear periphery (50%); and (3) mixed nuclear and cytoplasmic localisation (33.4%). In all sera ANCA fluorescence colocalised almost completely with apoptotic DNA, with persistence of a diVuse and intense fluorescence. No significant changes in ANCA titres were found in non-apoptotic neutrophils. Conclusions-The antigen(s) of ANCA associated with ulcerative colitis seems to be localised in most cases in the neutrophil nucleus. The almost identical colocalisation of ANCA and apoptotic cleaved DNA suggests that intracellular DNA redistribution during neutrophil apoptosis may play a role in antigen exposure to the immune system and ANCA production in ulcerative colitis. (Gut 2000;47:74-78)

show abstract

“…An association of atypical pANCA with a more refractory type of disease and with the development of pouchitis after colectomy has been described [30,[36][37][38][39]. The association with pouchitis, however, has not been found in other studies [40,41]. In CD, ANCA have been found in a subgroup of patients, termed UC-like CD, characterized by left-sided colonic inflammation, rectal bleeding, mucus discharge, urgency and treatment with topical agents [42].…”

Section: Auto-antibodies Atypical Pancamentioning

confidence: 97%