Antinociceptive activity and chemical composition of Wei–Chang–An–Wan extracts

Abstract

Context: Currently, famous traditional Chinese medicine formulas have undergone re-evaluation and development in China. Wei-Chang-An-Wan (WCAW) as one of them has been used for treating various gastrointestinal diseases for several decades. The secondary development of WCAW is in progress so as to interpret the effective material basis or find new pharmacological activity. Objective: To evaluate the antinociceptive effect of methanol extract of WCAW (ME) as well as four fractions (P.E., EtOAc, n-BuOH, H 2 O) and obtain information on the correlation between the contents of the fractions and antinociceptive effect. Materials and methods: ME was divided into four parts extracted by petroleum ether, ethyl acetate and n-butanol. Antinociceptive activity was evaluated by three models of acetic acid-induced writhing, formalin and hot-plate test in mice after repetitive administration of ME at 200, 400 or 800 mg/kg, P.E. 132 mg/kg, EtOAc 106 mg/kg, n-BuOH 176 mg/kg and H 2 O 176 mg/kg for six days. The chemical compounds were analyzed by HPLC-ESI-MS. Results: ME at 800 mg/kg inhibited acid-induced writhing by 84.69%, and reduced the licking time of second phase in formalin test by 53.23%. The inhibition rates in acid-induced writhing of P.E., EtOAc, n-BuOH and H 2 O were 27.79, 33.85, 38.97 and 37.69%, respectively, and in formalin test about 50%. They had no effect on the hot-plate test. HPLC-ESI-MS analysis showed that 68 chemical compounds were detected and 41 compounds were identified from ME. Discussion and conclusion: The results obtained herein indicate that WCAW possesses the antinociceptive activity that provides a new aspect in clinical application.

Gastrointestinal effect of methanol extract ofRadix Aucklandiaeand selected active substances on the transit activity of rat isolated intestinal strips

“…Weichang'an Pill, a traditional Chinese medicine (TCM) formula, has been used to treat irritable bowel syndrome and functional diarrhea for several decades (Hu et al, 2009;Liu et al, 2013;Wang et al, 2012). As a main component in Weichang'an pill, RA accounts for 25% of the prescription.…”

Based on UPLC-Q-TOF-MS/MS, Systematic Network Pharmacology, and Molecular Docking to Explore the Potential Mechanism of Fructus Aurantii for Major Depression Disorder

“…Fructus Aurantii (FA) is the dry immature fruit of the Citrus Aurantium L. It often used as a Chinese medicine for regulating qi and used in clinical practice and is one of the components of the most common Chinese medicine prescriptions, including Chaihu Shugan San and Weichang Anwan [8,9]. e main active ingredients of the FA include flavonoids, coumarins, volatile oils, and alkaloids, which exert an antidepressant effect [10], lower the cholesterol [11], have antitumor effect [12], promote the gastrointestinal power [13], and have other pharmacological activities.…”

Potential Metabolic Drug–Drug Interaction of Citrus aurantium L. (Rutaceae) Evaluating by Its Effect on 3 CYP450

“…Fructus aurantii (FA, Zhiqiao in Chinese), the dried and immature fruit of Citrus aurantium L. ( Rutaceae ) or its cultivars gathered from July, is used as an expectorant and digestant herb in traditional Chinese medicine, which is cited in Chinese Pharmacopoeia (1 part) of 2015 version. It is widely used in clinic as one component of several formulae, such as Xue-Fu-Zhu-Yu Tang ( Liu et al, 2004 ), decoction of Bulpleurum falcatum L. and FA ( Li et al, 2004 ), the formulae of FA and Magnolia bark which has produced relatively favorable effect to functional dyspepsia ( Huang et al, 2011 ; Xiong et al, 2015 ), Chaihu-Shugan-San ( Fan et al, 2012 ), and Wei-Chang-An-Wan ( Liu et al, 2013 ). FA extract is reported to have a wide range of potential therapeutic effects, such as hypocholesterolemic action ( Kim et al, 2011 ), anti-tumor action ( Zhou et al, 2007 ), prokinetic action ( Huang et al, 2011 ; Zhang et al, 2012 ), anti-ischemic action ( Kang et al, 2007 ), and anti-depressive action ( Fan et al, 2012 ; Zhang et al, 2012 ).…”