Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice

Figueiredo, Jozi Godoy; Bitencourt, Flávio da Silveira; Beserra, Ingrid Gonçalves; Teixeira, C.S.; Luz, Patrícia Bastos; Bezerra, Eduardo Henrique Salviano; Mota, Mário Rogério Lima; Assreuy, Ana Maria Sampaio; Cunha, Fernando de Queiróz; Cavada, Benildo S.; Alencar, Nylane Maria Nunes de

doi:10.1007/s00210-009-0448-2

Cited by 21 publications

(24 citation statements)

References 45 publications

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“…The involvement of the opioid system in lectin activity has already been demonstrated, as in the naloxone‐induced reversion of the antinociceptive effect of lectins from Bryothamnion seaforthii , Bryothamnion triquetrum , and C. boliviana [4,7] and the opioid regulation of hyperinsulinemia by the lectin from Canavalia ensiformis (which is 99% homologous with ConBr) [41].…”

Section: Discussionmentioning

confidence: 99%

“…First described for algal lectins [4], antinociceptive effects were subsequently observed in mice injected intravenously with leguminous lectins. Thus, lectins extracted from Lonchocarpus sericeus and Canavalia grandiflora inhibited mechanical hypernociception [5,6], while the lectin of Canavalia boliviana inhibited central and peripheral nociception via the opioid system [7]. In another recent study from our group, Diocleinae lectins ( Canavalia maritima , C. grandiflora , Dioclea guianensis , Dioclea violacea, and Cratylia floribunda ) produced antinociceptive effects inhibiting acetic acid–induced writhing in mice when administered orally [8].…”

Section: Introductionmentioning

confidence: 99%

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Opioid‐like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis

Pires

Assreuy

Lopes

et al. 2011

Fundamemntal Clinical Pharma

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The objective of this study was to evaluate the antinociceptive effects of a lectin from Canavalia brasiliensis (ConBr) when administered orally to murine models of chemical and thermal nociception. ConBr up to 100 mg/kg produced significant and dose-dependent antinociceptive effects: 81% reduction in abdominal writhing induced by 0.6% acetic acid; 26 and 52% reduction in early- and late-stage paw licking, respectively, induced by 2.5% formalin; and 155% increase in reaction latency (heightened thermal pain threshold). In all models, the antinociceptive effect was reversed by the lectin-binding carbohydrate α-d-methyl-mannoside and by the nonselective opioid antagonist naloxone. The antinociceptive effect observed in the formalin test was inhibited by the δ-selective antagonist naltrindole and the κ-selective antagonist nor-binaltorphimine but not by the μ-selective antagonist cyprodime. In conclusion, when administered orally to Swiss mice, the ConBr lectin displayed antinociceptive activity, both peripheral and central, mediated by the opioid system and involving δ-and κ-receptors and the lectin domain.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Opioid‐like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis

Pires

Assreuy

Lopes

et al. 2011

Fundamemntal Clinical Pharma

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“…The referred experimental model has been widely applied to verify analgesic activity promoted by lectins present in vegetal species (Holanda et al, 2009) and algae , as observed in a lectin-like protein purified from Clitoria fairchildiana seeds, in which dose-dependent antinociceptive activity was also reported (Leite et al, 2012). The lectinic site seems to be involved significantly on the lectin-promoted antinociceptive activity, as reported in the analgesic agglutinins isolated from C. boliviana seeds and the red algae Pterocladiella capilacea, in which previous association of these lectins with their specific carbohydrates (glycose and mucin) blocked the protein from exert its function against the painful stimulus, resulting in no contortion inhibition (Figueiredo et al, 2009;Silva et al, 2010).…”

Section: Acetic Acid-induced Abdominal Contortion Modelmentioning

confidence: 88%

“…The effect of lectins on painful stimuli has presented wide variation of stimulated receptors. The algae lectins appear to act mainly on the peripheral nervous system (Bitencourt et al, 2008;Vanderlei et al, 2010), and the legume lectins appear to act at the Central Nervous System (de Freitas Pires et al, 2013;Figueiredo et al, 2009), although it is not a universal claim. Figure 6.…”

Section: Formalin Methodsmentioning

confidence: 99%

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

Lacerda¹,

Moreira²,

Nascimento³

et al. 2015

J. Med. Plants Res.

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Lectins are proteins present in all living beings capable of interacting specifically and reversibly to carbohydrates or glycoconjugates form. They stand out in this family of lectins legumes, which are of special scientific interest because they have different biological functions. This study investigated the presence of a lectin in Brazilian seeds of Mucuna, proceeding with their isolation and evaluation of its antinociceptive effects, anti-inflammatory and antihemolytic activity. Saline extracts of Mucuna pruriens were investigated for the presence of hemagglutinating activity by serial dilution and applied to Sephacryl S-200HR columns and DEAE Sephacel HiPrepFF to purify their lectin. After assessing the purity and molecular weight by polyacrylamide agarose gel electrophoresis (PAGE), the hemolytic activity on human erythrocytes was determined, as well as its antinociceptive action by models of writhing induced by acetic acid and formalin, in addition to its anti-inflammatory activity by testing the paw edema induced by carrageenan. The results indicated that Brazilian Mucuna seeds had a lectin that was purified from seeds having molecular weight of 60.0 kDa and only able to agglutinate erythrocytes of rabbit. The lectin showed an antihemolytic effect on human erythrocytes by not causing hemolysis in these cells compared to the negative control. The administration of lectin in rodents intraperitoneally inhibited the writhing by 99% (2.5 mg/kg), induced by acetic acid, as well as in the method where the formalin nociceptive stimulus was reduced by 51% in stage one and 77% in stage two of the test, using 2.5 mg/kg lectin. The anti-inflammatory activity demonstrated a decrease in paw edema induced by carrageenan in 72% better result than indomethacin control where there was a reduction of only 48% of edema. The study supports the presence of a lectin in Brazilian seeds of M. pruriens with antinociceptive, anti-inflammatory and antihemolytic activities.

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“…A considerable number of marine invertebrate lectins have already been purified and characterized, and considerable attention is focused on its biological recognition role [8][9][10][11][12]. The Table I summarizes the origin, specificity, and biological activity from some lectins [5,[17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35]. Then, they come as ancestral molecules that show a high degree of conservation during the evolution to superior vertebrates [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Holothuria grisea agglutinin (HGA): the first invertebrate lectin with anti‐inflammatory effects

Moura

Aragão

Melo

et al. 2012

Fundamemntal Clinical Pharma

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Holothuria grisea agglutinin (HGA) is a dimeric lectin of molecular mass 228 kDa by gel filtration with monomers of 105 kDa by SDS-PAGE. The lectin is highly thermostable as it retains full activity for 1 h at 70 °C. Unlike other lectins purified from marine invertebrates, the hemagglutination activity of HGA does not require any divalent metal ions. The affinity analysis of HGA showed that only mucin was able to inhibit the hemagglutinating activity. HGA administered intravenously was tested in classical models of nociception and inflammation. HGA was able to inhibit neutrophil migration into the peritoneal cavity induced by carrageenan. This inhibitory effect was 68% at a dose of 1 mg/kg. In acetic acid-induced writhing tests, a significant antinociceptive effect was observed by treatment with HGA (0.1; 1 or 10 mg/kg) reducing constrictions by 27, 90 and 84%, respectively. In formalin tests, HGA at a dose of 10 mg/kg showed antinociceptive effect only in the inflammatory phase (phase 2). Nevertheless, in hot-plate tests, HGA did not show any nociceptive effect. In rota-rod and open-field tests, HGA did not alter the animals' behavior. The treatment with HGA 10 mg/kg presented diminished myeloperoxidase activity activity (81.6% inhibition) and raised the circulating levels of NO by 50.4% when compared with the carrageenan group. HGA has demonstrated the ability to modulate the inflammatory response in models of inflammation in vivo. HGA is the first marine invertebrate lectin that showed an anti-inflammatory effect. This finding opens a new perspective on the potential of lectins from the marine environment.

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Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice

Cited by 21 publications

References 45 publications

Opioid‐like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis

Opioid‐like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

Holothuria grisea agglutinin (HGA): the first invertebrate lectin with anti‐inflammatory effects

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