Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats

Abstract

Aim:The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.Subjects and Methods:Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated. The antinociceptive activity of purified fractions was investigated using formalin and hot plate test. Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.Results:In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg. In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism. Sw FrIV and Ho FrIV significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration at 10 mg/kg, respectively. In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).Conclusion:Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model. However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.

Sargassum Seaweed as a Source of Anti-Inflammatory Substances and the Potential Insight of the Tropical Species: A Review

“…Studies on the anti-inflammatory activity of the Sargassum crude sulfated polysaccharides (CSP) have been carried out by several research groups [39,96,97,98,99,100,101,102,103,104,105,106,107] and are shown in Table 2. Sulfated polysaccharides (SP), such as fucoidan, carrageenan, and ulvan, are recognized as the typical functional compounds derived from various types of marine algae.…”

Sulfated polysaccharides of seagrass Halophila ovalis suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line

“…[12] Similarly, we reported the existence of low-molecular-weight sulfated galactan from seagrass H. ovalis and characterized its structural and functional properties. [13] The monosaccharide composition analysis of sulfated galactans revealed the presence of galactose, xylose, fructose, mannose, fucose, glucose, and arabinose. Similarly, in seagrass Cymodocea nodosa , galactose and mannose were found to be the major sugar components.…”

“…The methodology used for the sequential extraction and purification of polysaccharide was adapted according to our previous study described earlier. [13] The extraction mode includes three main steps – depigmentation, precipitation, and extraction of the polysaccharide with hot water. Purification was achieved in a gradient manner using sodium chloride as an eluant.…”

“…In our previous study, we reported the physicochemical characteristics and existence of the antinociceptive and anti-inflammatory activities of the sulfated polysaccharide fractions extracted from seagrass H. ovalis in vivo. [13] The present study was undertaken to highlight for the first time the antioxidant, cytotoxic effects on peripheral blood mononuclear cells (PBMCs), and cytokine IL-8 secretion on HT-29 cell lines in vitro .…”

Seaweeds: An Extensive Review of Medicinal Values

“…Carrageenan has been widely used as a tool in the screening of novel antiinflammatory drugs [364]. Among others, Porphyra vietnamensis [365,366], Eucheuma Cottonii [367], Dichotomaria obtusata ( Figure 17) [368], Cystoseira sedoides, Cladostephus spongiosis, Padina pavonica ( Figure 18) [369], Ecklonia cava (due to phlorotannins) [370][371][372], Caulerpa racemose [373], Sarcodia ceylanica [374], actinotrichia fragilis [375], Dictyota menstrualis ( Figure 19) [376], Gracilaria cornea [377], Gracilaria birdiae [378], Class Phaeophyceae, Rhodophyceae and Chlorophyceae [379], Caulerpa curpressoides [380,381], Ulva lactuca ( Figure 20) [382], Sargassum wightii and Halophila ovalis [383], Grateloupia lanceolate [384], Sargassum fulvellum and Sargassum thunbergii [385], Briareum excavatum [386], Caulerpa racemose [387], Sargassum hemiphyllum [388], Laurencia obtusa [389], Caulerpa kempfii [390] Caulerpa cupressoides [391] are reported for their analgesic and anti-inflammatory properties.…”