Antinociceptive and anti-inflammatory effect of Lippia pedunculosa Hayek essential oil and its β-cyclodextrin inclusion complex

Abstract

Lippia pedunculosa Hayek (EOLp) presents tripanocid and amebicid effects. However essential oil needs to be further studied in experimental models of analgesia and inflammation once the prevalence of pain in the population generates great suffering and disability and the drugs most often used have undesirable side effects. We also evaluated whether the inclusion complex formulation EOLp/β-cyclodextrin (β-CD) was able to improve the antinociceptive activity of the EOLp alone. Data were evaluated by analysis of variance (ANOVA), followed by Tukey’s test. Differences were considered significant if p<0.05. EOLp presented better antinociceptive effect when compared to the EOLp/β-CD inclusion complex. Thus, cyclodextrins appear not to be efficient for essential oils with peroxide substances. However, in peritonitis, EOLp reduced total leucocyte migration and IL-1β levels in the peritoneal fluid, which confirmed its anti-inflammatory effect. The observed effects suggest that EOLp is the best promising option for the treatment of inflammation and pain-related disorders.

A 9-aminoacridine derivative induces growth inhibition of Ehrlich ascites carcinoma cells and antinociceptive effect in mice

“…First, motor coordination was assessed by the rota-rod test ( Fajemiroye et al, 2018 ). The rota-rod test is important to ensure that the drug’s antinociceptive effects are not due to neurotoxicity, depression, or non-specific relaxation of the Central Nervous System (CNS), avoiding false-positive results ( Quintans-Júnior et al, 2010 ; Braga et al, 2021 ). Our results showed no changes in motor coordination in mice after ACS-AZ treatment; thus, ACZ-AZ activity in the nociception tests cannot be attributed to myorelaxant or CNS sedative effects.…”

“…Thermal stimulation activates nociceptors (non-myelinated C fibers), which transmit information to specific regions in the CNS, producing an organized nociceptive response ( Sulaiman et al, 2009 ; Mekonnen et al, 2010 ; Singh et al, 2018 ). For this reason, this experimental model is used to assess the effects of opioid, sedative, and hypnotic drugs ( Yeung et al, 1977 ; Braga et al, 2021 ). Similarly to morphine, we observed that ACS-AZ increased the time latency between the contact of the animal’s hind paw with the hot plate and the paw-licking reaction or jumping off the surface.…”