Antinociceptive and anti-inflammatory effects of the ethyl acetate stem bark extract of Bridelia scleroneura (Euphorbiaceae)

Abstract

Bridelia scleroneura is a member of the Euphorbiaceae family. In folk medicine in Cameroon, the stem bark of this plant is used for relieving abdominal pain, contortion, arthritis and inflammation. In this study, the antinociceptive and anti-inflammatory activities of the ethyl acetate stem bark extract have been evaluated. The putative analgesic effect of the plant extract was examined in abdominal constriction, hot plate, formalin and on pain using tail immersion mouse models and in carrageenan-induced paw edema in rats. The extract (150-600 mg/kg) exhibited a dose-dependent analgesic effect (46.27-78.97%) in acetic acid-induced abdominal constriction in mice. B. scleroneura extract increased the pain latency of nociceptive response to thermal stimuli at the higher dose of 600 mg/kg. B. scleroneuna induced significant dose-dependent reduction of the nociception in both early and late phases of the formalin test. The extract at the dose of 300 mg/kg, increased significantly, by 63.70% and 52.01% the tail-immersion latency time, 1 and 2 h post-dosing. In the carrageenan test, B. scleroneura (150-600 mg/kg, p.o) had dose-dependent and significant effects at different time intervals. This behaviour was similar to indometacin (10 mg/kg) used as a standard drug. These results show that the ethyl acetate stem bark extract of B. scleroneura possesses peripheral and central analgesic properties as well as anti-inflammatory activity against acute inflammation processes, in support of the folk medicinal use of the plant.

Phytochemical Constituents of Citrus hystrix DC. Leaves Attenuate Inflammation via NF-κB Signaling and NLRP3 Inflammasome Activity in Macrophages

“…Lupeol, a pentacyclic lupane-type triterpene contains significant biological properties such as antiprotozoal activity [27,28], anticancer [29], antimicrobial activity [30] and anti-inflammation [31]. This triterpene is found in various types of plants such as Bridelia scleroneura [32], Millettia versicolor [33] and Euclea natalensis [27]. Our study showed that C. hystrix leaves are a lupeol source, concurring with the findings of Anuchapreeda et al [34].…”

Neoboutonia melleri var velutina Prain: in vitro and in vivo hepatoprotective effects of the aqueous stem bark extract on acute hepatitis models

“…We observed decreased TNFα and IL-8 expression after NVH treatment. Our findings indicate an NVH anti-inflammatory potential in line with anti-inflammatory properties reported in other Euphorbiaceae plant family members [ 25 ]. This anti-inflammatory effect appears to be significant only at 10 μg/mL suggesting an optimal efficient dose of NVH between 1 and 10 μg/mL.…”

Anti-inflammatory, analgesic and antipyretic activity of aqueous extract of fresh leaves of Coccinia indica

“…Further the anti-inflammatory activity could also be associated with antioxidant activity of C. indica (Venkateshwaran and pari 2003a, b) since inflammatory activity of carrageenan has been demonstrated to be accompanied by elevation in the free radicals level (Gamache et al 1986). The triterpenes, lupeol and b-sitosterol have been reported to exert anti-inflammatory activities (Theophile et al 2006). Therefore, this effect may also be attributed to these constituents reported to be present in leaves of C. indica (Rastogi and Mehrotra 1998;Ray and Kundu 1987).…”