Antinociceptive and anti-inflammatory effects of Memora nodosa and allantoin in mice

Florentino, Iziara Ferreira; Silva, Daiany P.B.; Galdino, Pablinny Moreira; Lino, Roberta Campos; Martins, José Luís Rodrigues; Silva, Dayane M.; Paula, José Realino de; Tresvenzol, Leonice Manrique Faustino; Costa, Élson Alves

doi:10.1016/j.jep.2016.04.010

Cited by 45 publications

(22 citation statements)

References 23 publications

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“…The beneficial effects of allantoin on inflammation, oxidative activities, and wound healing have been well known (8,9). In this study, we showed that allantoin improved gastritis, mucosal thickness, and cell numbers after ethanol-induced gastritis, decreased leukocyte, and vessel numbers and increased the PGE2 and NP-SH levels in the gastric tissue of male rats.…”

Section: Discussionsupporting

confidence: 51%

“…It has also been reported that allantoin decreases IL-4, IL-5, Ig-E levels, and the number of leukocyte cells in the ovalbumin-induced lung inflammation (11). Florentino et al (8) revealed that allantoin has anti-nociceptive and anti-inflammatory effects on the formalin-induced nociception test. Moreover, it has been shown that allantoin activates imidazoline I1-receptor (I1R) in animal and cell line studies (5,22).…”

Section: Introductionmentioning

confidence: 95%

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Allantoin improves histopathological evaluations in a rat model of gastritis

2018

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Purpose Gastritis is found to be one of the most common gastrointestinal diseases worldwide. However, current therapeutic agents cause side effects, interaction, and recurrence. Allantoin has anti-inflammatory and wound healing properties. In this study, the therapeutic effect of allantoin has been assessed on the histopathological indices and gastric mucosal barrier of male rats. Methods Male rats were equally divided into control, ethanol-induced gastritis, and allantoin groups. The therapeutic groups consisted of gastritis plus 12.5 mg/kg allantoin, gastritis plus 25 mg/kg allantoin, and gastritis plus 50 mg/kg allantoin groups. After 5 days of allantoin administration, the rats were sacrificed and a part of their gastric tissue was maintained at −70 °C for prostaglandin E2 (PGE2) and non-protein sulfhydryl (NP-SH) measurements. Another part was stained with hematoxylin and eosin and Masson’s trichrome. Results We found that Allantoin increased parietal and mucosal cell counts and mucosal thickness after gastritis induction. In addition, the number of leukocytes and vessels decreased in both of the mucosal and the submucosal layers. Allatoin improved gastric ulcer in all treatment groups. Gastric levels of PGE2 and NP-SH increased after allantoin treatment. Conclusion This study indicated that allantoin had a considerable effect on gastritis treatment, which seems to result from the reinforcement of gastric mucosal barrier.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 95%

Allantoin improves histopathological evaluations in a rat model of gastritis

2018

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“…The mice were placed in glass beakers and observed for 30 min. The duration of licking and biting of the paw was observed for two phases-early phase (0-5 min) and late phase (15-30 min) [23]. The percentage of licking and biting inhibition was calculated by the following formula:…”

Section: Formalin Testmentioning

confidence: 99%

A mechanistic approach to HPLC analysis, antinociceptive, anti-inflammatory and postoperative analgesic activities of panch phoron in mice

Gias

Afsana

Debnath

et al. 2020

BMC Complement Med Ther

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Background: Panch phoron is a mixture of five spices containing an equal proportion of Foeniculum vulgare (fennel), Trigonella foenum-graecum Linn (fenugreek), Nigella sativa (black cumin), Cuminum cyminum (cumin) and Brassica nigra (black mustard). The mixture is commonly used in Bangladeshi cuisine and possesses many pharmacological effects. In this study, we evaluated the antinociceptive and anti-inflammatory activities of aqueous panch phoron extract (PPE) in vivo, its possible mechanism of action and phytochemical analysis by High-Performance Liquid Chromatography (HPLC). We also investigated the effect of PPE on postoperative pain in mice. Methods: HPLC was carried out using LC-20A Modular HPLC system to identify the bioactive compounds present in PPE. Five groups of Swiss albino male mice (n = 6 per group) were orally treated with 10 ml/kg of distilled water or 10 mg/kg of sodium diclofenac or three doses of PPE (100 mg/kg, 300 mg/kg, 500 mg/kg). In vivo assessment was carried out by the writhing test, tail-flick test, formalin test, and carrageenan induced paw edema test. The opioid antagonist, naloxone was used in the acetic acid test to evaluate the involvement of opioid receptors. To assess the effect of PPE in postoperative pain, mice that underwent sciatic nerve surgery were measured for the paw withdrawal latency in a hot water bath. Results: In HPLC analysis, different types of phenolic compounds and flavonoids, including catechin hydrate, paracoumaric acid, vanillic acid, and syringic acid were detected. Treatment with PPE exhibited dose-dependent antinociceptive and anti-inflammatory activities in pain models (p < 0.05). Furthermore, naloxone did not reverse the effect of PPE in the writhing test. Mice that underwent sciatic nerve surgery showed that the paw withdrawal latency increased gradually over 7 days. Conclusions: Our results demonstrate that PPE has significant antinociceptive and anti-inflammatory activities and can provide significant postoperative analgesia.

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“…Rats treated with allantoin at a higher dose (400 mg/kg po) exhibited improved locomotor activity and nerve velocity but the treatment had no significant effect on cold and thermal hyperalgesia, mechano‐tactile allodynia and mechanical hyperlgesia. This may be because of the peripheral antinociceptive activity of allantoin, which has been reported to involve activation of opioid receptor‐mediated ATP‐sensitive channels . Thus at a high dose (400 mg/kg) allantoin may exhibit analgesic activity.…”

Section: Discussionmentioning

confidence: 99%

“…The levels of circulating GLP‐1 have been found to correlate with the concentration of systemic IL‐6 and also with the concentrations of other markers of inflammation, suggesting that the regulation of GLP‐1 is inflammation dependent. It is thus worth noting that treatment with allantoin may promote the release of GLP‐1 and improve the function of β‐cells, thereby maintaining insulin and glucose levels …”

Section: Discussionmentioning

confidence: 99%

Allantoin attenuates deficits of behavioural and motor nerve conduction in an animal model of cisplatin‐induced neurotoxicity in rats

S¹,

Mohamad²,

Razdan³

2019

Anim Models and Exp Med

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Background The aim of the present study was to evaluate the neuroprotective effect of allantoin in cisplatin‐induced toxicity in rats. Methods Adult male Wistar rats weighing 160‐200 g were used. Neuropathy was induced by injecting cisplatin (2 mg/kg, ip, twice a week for 6 weeks) and the rats were concurrently treated with allantoin (200 and 400 mg/kg, po) for 8 weeks. At the end of the study, body weight and hemogram were measured. Behavioural tests were performed, including tests for cold and hot hyperalgesia, motor co‐ordination, locomotor activity, mechano‐tactile allodynia and mechanical hyperalgesia. The rats were then sacrificed and sciatic nerve conduction velocity was determined. The antioxidant enzyme and nitric oxide levels in sciatic nerve homogenates were measured. Results In this study, allantoin restored the motor nerve conduction velocity deficits induced by cisplatin, and the allantoin‐treated rats showed improvement in cold and thermal hyperalgesia, mechano‐tactile allodynia, and mechanical hyperalgesia. Allantoin treatment also improved the rats’ hematological status, increasing haemoglobin, platelet and RBC counts compared to the cisplatin‐treated group. Allantoin treatment also mitigated the functional abnormalities seen in the cisplatin neuropathy group, protecting neurons from the neurotoxic effects of cisplatin. Conclusion Allantoin shows promise for use as an adjuvant drug in cancer treatment to protect against cisplatin‐induced neuropathy.

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Antinociceptive and anti-inflammatory effects of Memora nodosa and allantoin in mice

Cited by 45 publications

References 23 publications

Allantoin improves histopathological evaluations in a rat model of gastritis

Allantoin improves histopathological evaluations in a rat model of gastritis

A mechanistic approach to HPLC analysis, antinociceptive, anti-inflammatory and postoperative analgesic activities of panch phoron in mice

Allantoin attenuates deficits of behavioural and motor nerve conduction in an animal model of cisplatin‐induced neurotoxicity in rats

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