Antinociceptive and anti-inflammatory effects of Cuphea aequipetala Cav (Lythraceae)

Alonso-Castro, Ángel Josabad; Arana-Argáez, Víctor; Yáñez-Barrientos, Eunice; Ramírez-Camacho, Mario Alberto; Wróbel, Katarzyna; Torres‐Romero, Julio César; León-Callejas, Carolina

doi:10.1007/s10787-020-00709-3

Cited by 14 publications

(9 citation statements)

References 25 publications

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“…On the other hand, some studies, suggest that, ROS and NO molecules are essential for parasite proliferation and growth, due to provide ideal conditions (e.g., iron availability in macrophages) for parasite-replication 52,53 . In the present study, the amounts of NO and H 2 O 2 molecules reported are similar to those reported in previous studies 36,54,55 , which peritoneal murine macrophages were stimulated with extracts of plants or microorganisms with antiparasitic activity. According to those data, toxic effects were not detected, therefore, we believe that the amounts of molecules produced by the TSA-1-C4 plus Tc24-C4 bivalent recombinant strategy is appropriate.…”

Section: Discussionsupporting

confidence: 91%

TSA-1-C4 plus Tc24-C4 recombinant protein stimulation induce immunomodulatory activity by activation of macrophages

Dzul-Huchim

Alfaro-Chacón

Novelo-Sarabia

et al. 2023

Preprint

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Chagas disease is a chronic infection caused by Trypanosoma cruzi. The first-line drugs approved for treatment have several limitations and are associated with toxicity. The recombinant TSA-1-C4 and Tc24-C4 proteins have been profiled as promising candidates for the formulation of therapeutic vaccines, leading us to propose them in combination as a bivalent recombinant protein strategy. In this study, we evaluated the immunomodulatory effect of TSA-1-C4 plus Tc24-C4 recombinant protein combination by in-vitro assays. Macrophages from naïve BALB/c mice were isolated and stimulated with TSA-1-C4+Tc24-C4 proteins, supernatants were recovered to measure NO, H O , and, TNF-α, IL-1β, IL-6 and IL-10 cytokines. Stimulated macrophages were co-cultured with CD8 T cells from naïve mice, and inflammatory cytokine-profile was measured from supernatants. Additionally, by experimental T. cruzi acute infection model, cytotoxicity was evaluated in mice after the treatment with TSA-1-C4+Tc24-C4 proteins in presence of a TLR-4 agonist adjuvant, E6020-SE. We observed that the bivalent recombinant protein strategy activates macrophages by NO and H O production, also induced a significant Th1 immune-response compared to either TSA-1-C4 or Tc24-C4 stimulated macrophages. Moreover, naïve CD8 T cells in presence of TSA-1-C4+Tc24-C4 stimulated-macrophages similarly boosted Th1 profile by significant production of IFN-γ and TNF-α cytokines. These results support the synergistic effect of the bivalent recombinant protein strategy, which leads activation of peritoneal macrophages and CD8 T cells eliciting the Th1 immune response. Although the administration of TSA-1-C4+Tc24-C4+E6020-SE showed cytotoxic activity in T. cruzi-infected mice, there was not a benefit compared to stimulate with TSA-1-C4 or Tc24-C4 antigens formulated with E6020-SE adjuvant.

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Section: Discussionsupporting

confidence: 91%

TSA-1-C4 plus Tc24-C4 recombinant protein stimulation induce immunomodulatory activity by activation of macrophages

Dzul-Huchim

Alfaro-Chacón

Novelo-Sarabia

et al. 2023

Preprint

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“…Cuphea species in recent research displayed anti‐inflammatory potential. A study with ethanol extract from the leaves and stem of C. aequipetala showed in vitro and in vivo anti‐inflammatory effects by increasing the release of IL‐10 [23] . In another study, an inhibitory effect on the PMNs migration was observed, mainly for C. calophylla and C. racemosa [12] .…”

Section: Resultsmentioning

confidence: 94%

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Santos

Soares

Beltrame

et al. 2021

Chemistry & Biodiversity

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The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves. The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization. The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001 -10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils. The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition. From the nineteen proposed compounds, six of them are described for the first time in this genus. The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations. The ACE-inhibition presented results ranging from 19.58 to 22.82 %. In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential. Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.

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“…Recently, neuroinflammation has been focused on as an important regulator in the pathology of neuropathic pain [ 23 ]. Besides, the overexpression levels of proinflammatory factors were observed in the spinal cord of neuropathic pain animal models, and the administration of anti-inflammatory agents inhibited inflammatory responses and alleviated neuropathic pain [ 24 , 25 ]. NF- κ B is a vital transcription factor, plays an important role in regulating the gene expression of inflammatory factors, and is involved in the initiation, persistence, and severity of neuropathic pain following nerve impairment [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Gastrodia elata Blume Polysaccharides Attenuate Vincristine-Evoked Neuropathic Pain through the Inhibition of Neuroinflammation

Xie

Chen

et al. 2021

Mediators of Inflammation

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Vincristine (Vin) is a well-known antitumor agent that frequently evokes neuropathic pain and decreases the quality of life of patients. Polysaccharides (GBP) extracted from Gastrodia elata Blume have been demonstrated to possess anti-inflammatory and neuroprotective effects in vivo; however, the effects of GBP on Vin-induced neuropathic pain remain unknown. The present study is aimed at exploring the alleviative potential of GBP against chemotherapy-evoked peripheral neuropathy to better understand and extend its pharmacological application. Vin was administered intraperitoneally to evoke neuropathic pain. GBP was orally administered for 21 days. The mechanical allodynia and thermal hyperalgesia were assessed using the Von Frey test and hot-plate test. Histopathological changes were assessed by hematoxylin and eosin staining. ELISA kits were used to measure the levels of inflammatory cytokines in the sciatic nerve, spinal cord, and dorsal root ganglion (DRG). qRT-PCR was employed to examine the expression of inflammatory cytokines and Sirtuin1 (SIRT1) in the sciatic nerve, spinal cord, and DRG. Our findings revealed that GBP treatment enhanced the paw withdrawal latency and paw withdrawal threshold and restored Vin-induced sciatic nerve damage in rats. GBP also attenuated the Vin-induced increase of proinflammatory cytokine levels, including IL-6, IL-8, TNF-α, IL-1β, and NF-κB. On the molecular level, treatment with GBP downregulated the mRNA levels of IL-6, IL-8, TNF-α, and IL-1β in the sciatic nerve, spinal cord, and DRG. Meanwhile, GBP increased SIRT1 activity and mRNA expression levels. Our data indicated that GBP exerted a potential protective effect against chemotherapy-induced neuropathic pain which might be mediated via the inhibition of neuroinflammation.

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Antinociceptive and anti-inflammatory effects of Cuphea aequipetala Cav (Lythraceae)

Cited by 14 publications

References 25 publications

TSA-1-C4 plus Tc24-C4 recombinant protein stimulation induce immunomodulatory activity by activation of macrophages

TSA-1-C4 plus Tc24-C4 recombinant protein stimulation induce immunomodulatory activity by activation of macrophages

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Gastrodia elata Blume Polysaccharides Attenuate Vincristine-Evoked Neuropathic Pain through the Inhibition of Neuroinflammation

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