Antinociceptive and Anti-Inflammatory Effects of Ethanol Extract from Vernonia polyanthes Leaves in Rodents

Abstract

The ethanol extract from Vernonia polyanthes leaves (EEVP) was investigated for antinociceptive and anti-inflammatory effects at the doses (p.o.) of 100, 200 and 400 mg/kg in animal models. The extract reduced the number of abdominal contortions by 16.75% and 31.44% at a dose of 200 and 400 mg/kg, respectively. The results obtained showed that EEVP exerted a significant antinociceptive effect in the two phases of formalin. The EEVP increased the reaction time on a hot plate at the doses of 100, 200 and 400 mg/kg after 90 min of treatment. The paw edema was reduced by EEVP at the doses of 100, 200 and 400 mg/kg after 4 h of application of carrageenan. Doses of 200 and 400 mg/kg, administered 4 h before the carrageenan injection, significantly reduced the exudate volume (29.25 and 45.74%, respectively) and leukocyte migration (18.19 and 27.95%, respectively). These results suggest that V. polyanthes can be an active source of substances with antinociceptive and anti-inflammatory activities.

Evidence of Bioactive Compounds from Vernonia polyanthes Leaves with Topical Anti-Inflammatory Potential

“…HEVP and EAEVP markedly inhibited the capsaicin-induced ear edema probably through the blockade of TRPV1 receptors by preventing the spread of painful stimuli. This data supported the previous report of the antinociceptive activity of V. polyanthes made by our research group [17]. Although dexamethasone is commonly used in this assay, it is not very effective in reducing the edema induced by capsaicin.…”

“…Among the many plants found in Brazilian biodiversity, Vernonia polyanthes Less, belonging to the Asteraceae family, has been traditionally used as a diuretic, hypotensive, antiulcerogenic, antirheumatic, antimicrobial, cicatrizing, cutaneous damage, anti-inflammatory [17], and for the treatment of malaria and fever [18]. Focusing on the pharmacological investigations, this species has shown antiulcer [19], antinociceptive, anti-inflammatory [17], antihypertensive [20], antibacterial [21] and antifungal [22] activities.…”

“…Focusing on the pharmacological investigations, this species has shown antiulcer [19], antinociceptive, anti-inflammatory [17], antihypertensive [20], antibacterial [21] and antifungal [22] activities. These properties can be related to the presence of compounds such as 3,7-dimethoxy-5,3′,4′-trihydroxyflavone, 3′,4′-dimethoxyluteolin, 3,5-di- O -( E )-caffeoylquinic acid, 4,5-di- O -( E )-caffeoylquinic acid, luteolin, quercetin, protocatechuic acid, quercetin-3- O -β-glucoside, apigenin, isorhamnetin, chrysoeriol-7- O -glycuronyl, and acacetin-7- O -glycuronyl; as well as sesquiterpenes lactones glaucolide A, piptocarphin A, and piptocarphin B, identified in V. polyanthes leaves [18,23].…”

“…These properties can be related to the presence of compounds such as 3,7-dimethoxy-5,3′,4′-trihydroxyflavone, 3′,4′-dimethoxyluteolin, 3,5-di- O -( E )-caffeoylquinic acid, 4,5-di- O -( E )-caffeoylquinic acid, luteolin, quercetin, protocatechuic acid, quercetin-3- O -β-glucoside, apigenin, isorhamnetin, chrysoeriol-7- O -glycuronyl, and acacetin-7- O -glycuronyl; as well as sesquiterpenes lactones glaucolide A, piptocarphin A, and piptocarphin B, identified in V. polyanthes leaves [18,23]. In addition, phytochemical screening of the ethanol extract from leaves showed the presence of flavonoids, tannins, coumarins, terpenoids, sterols, saponins, and alkaloids [17]. Natural compounds, such as flavonoids and terpenes, can act by different mechanisms of action, inhibiting the synthesis or expression of inflammatory mediators [24,25,26].…”

Molecular Docking and Molecular Dynamics Simulation Studies of Triterpenes from Vernonia patula with the Cannabinoid Type 1 Receptor

“…The aerial parts of VP have displayed antioxidant and anti-inflammatory activity [ 17 ]. Several Vernonia species, including VP, have also demonstrated antinociceptive and sedative properties [ 18 , 19 , 20 ]. The aerial parts of VP are known to contain flavonoids, simple phenolics, and terpenoids [ 21 , 22 , 23 ].…”