Antinociceptive and gastroprotective activities of Bocconia arborea S. Watson and its bioactive metabolite dihydrosanguinarine in murine models

Gaona-Tovar, Emmanuel; Estrada-Soto, Samuel; González-Trujano, María Eva; Vargas, David Martínez; Hernandez-Leon, Alberto; Narváez-González, Fernando; Villalobos‐Molina, Rafael; Almanza-Perez, Julio César

doi:10.1016/j.jep.2022.115492

Cited by 4 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dihydrosanguinarine (DHS) was one of the compounds identified in the AOE. The participation of the GABAergic system in the antinociceptive actions of DHS was recently reported [ 23 ]. DHS (100 mg/kg i.p.)…”

Section: Discussionmentioning

confidence: 99%

“…DHS (100 mg/kg i.p.) showed a moderate reduction in the ambulatory activity of mice, but no sedative, hypnotic, or neurotoxic effects were observed by an electroencephalographic analysis in mice [ 23 ]. The present study corroborates that DHS uses the GABAergic system for its pharmacological actions.…”

Section: Discussionmentioning

confidence: 99%

“…Each test was conducted 1 h after post-treatment administration. Preliminary pharmacological work carried out in the laboratory and the results obtained from the acute toxicity test conducted in this study on the AOE, as well as previous toxicological studies performed with DHS [ 20 , 23 ] allowed for the selection of doses for the AOE and DHS. The ED 50 value (the dose of AOE or DHS necessary to reach 50% of the pharmacological effect) was determined using linear regression analysis.…”

Section: Methodsmentioning

confidence: 99%

“…DHS exerted antinociceptive activity (ED 50 = 85 mg/kg) in the formalin test in mice without inducing gastric damage and inducing low toxicity (LD 50 > 2000 mg/kg i.p.). The antinociceptive activity of DHS was associated with the participation of GABAergic neurotransmission without showing sedative or neurotoxic effects [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Neuropharmacological Effects of the Dichloromethane Extract from the Stems of Argemone ochroleuca Sweet (Papaveraceae) and Its Active Compound Dihydrosanguinarine

Yáñez-Barrientos,

Barragan-Galvez,

Hidalgo-Figueroa

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

Argemone ochroleuca Sweet (Papaveraceae) is used in folk medicine as a sedative and hypnotic agent. This study aimed to evaluate the anxiolytic-like, sedative, antidepressant-like, and anticonvulsant activities of a dichloromethane extract of A. ochroleuca stems (AOE), chemically standardized using gas chromatography–mass spectrometry (GC–MS), and its active compound dihydrosanguinarine (DHS). The anxiolytic-like, sedative, antidepressant-like, and anticonvulsant activities of the AOE (0.1–50 mg/kg p.o.) and DHS (0.1–10 mg/kg p.o.) were evaluated using murine models. A possible mechanism for the neurological actions induced by the AOE or DHS was assessed using inhibitors of neurotransmission pathways and molecular docking. Effective dose 50 (ED50) values were calculated by a linear regression analysis. The AOE showed anxiolytic-like activity in the cylinder exploratory test (ED50 = 33 mg/kg), and antidepressant-like effects in the forced swimming test (ED50 = 3 mg/kg) and the tail suspension test (ED50 = 23 mg/kg), whereas DHS showed anxiolytic-like activity (ED50 = 2 mg/kg) in the hole board test. The AOE (1–50 mg/kg) showed no locomotive affectations or sedation in mice. A docking study revealed the affinity of DHS for α2-adrenoreceptors and GABAA receptors. The anxiolytic-like and anticonvulsant effects of the AOE are due to GABAergic participation, whereas the antidepressant-like effects of the AOE are due to the noradrenergic system. The noradrenergic and GABAergic systems are involved in the anxiolytic-like actions of DHS.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Neuropharmacological Effects of the Dichloromethane Extract from the Stems of Argemone ochroleuca Sweet (Papaveraceae) and Its Active Compound Dihydrosanguinarine

Yáñez-Barrientos,

Barragan-Galvez,

Hidalgo-Figueroa

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…Degradation and metabolism in the intestine may be one of the reasons for the low bioavailability of SAN. Although DHSA has also recently been reported to have antiinflammatory (Xiang et al, 2022) and analgesic (Gaona-Tovar et al, 2022) properties, whether it can regulate intestinal flora still needs to be elucidated. Several pharmacokinetic studies explain that SAN and CHE have low gastrointestinal absorption and bioavailability, and are rapidly metabolized in vivo (Vecera et al, 2007;Hu et al, 2019;Wu et al, 2020;Zhao et al, 2021).…”

Section: Groupsmentioning

confidence: 99%

Preclinical safety evaluation of Macleaya Cordata extract: A re-assessment of general toxicity and genotoxicity properties in rodents

Dong

Tang²,

Ma³

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Macleaya cordata extract (MCE) is widely used for its diverse pharmacological actions and beneficial effects on farm animals. Modern pharmacological studies have shown that it has anti-inflammatory, anti-cancer, and anti-bacterial activities, and is gradually becoming a long-term additive veterinary drug used to improve animal intestinal health and growth performance. Although some evidence points to the DNA mutagenic potential of sanguinarine (SAN), a major component of MCE, there is a lack of sufficient basic toxicological information on the oral route, posing a potential safety risk for human consumption of food of animal origin. In this study, we assessed the acute oral toxicity, repeated 90-day oral toxicity and 180-day chronic toxicity of MCE in rats and mice and re-evaluated the genotoxicity of MCE using a standard combined in vivo and ex vivo assay. In the oral acute toxicity test, the LD50 for MCE in rats and mice was 1,564.55 mg/kg (95% confidence interval 1,386.97–1,764.95 mg/kg) and 1,024.33 mg/kg (95% confidence interval 964.27–1,087.30 mg/kg), respectively. The dose range tested had no significant effect on hematology, clinical chemistry, and histopathological findings in rodents in the long-term toxicity assessment. The results of the bacterial reverse mutation, sperm abnormality and micronucleus test showed negative results and lack of mutagenicity and teratogenicity; the results of the rat teratogenicity test showed no significant reproductive or embryotoxicity. The results indicate that MCE was safe in the dose range tested in this preclinical safety assessment. This study provides data to support the further development of maximum residue limits (MRLs) for MCE.

show abstract

Vasorelaxant and tracheorelaxant effects of Bocconia arborea and their isolated benzophenanthridine alkaloids

Gaona-Tovar,

Estrada-Soto,

Ramírez-Hernández

et al. 2024

Fitoterapia

View full text Add to dashboard Cite

Antinociceptive and gastroprotective activities of Bocconia arborea S. Watson and its bioactive metabolite dihydrosanguinarine in murine models

Cited by 4 publications

References 41 publications

Neuropharmacological Effects of the Dichloromethane Extract from the Stems of Argemone ochroleuca Sweet (Papaveraceae) and Its Active Compound Dihydrosanguinarine

Neuropharmacological Effects of the Dichloromethane Extract from the Stems of Argemone ochroleuca Sweet (Papaveraceae) and Its Active Compound Dihydrosanguinarine

Preclinical safety evaluation of Macleaya Cordata extract: A re-assessment of general toxicity and genotoxicity properties in rodents

Vasorelaxant and tracheorelaxant effects of Bocconia arborea and their isolated benzophenanthridine alkaloids

Contact Info

Product

Resources

About