Antinociceptive Effect of the Extract of Morus nigra Leaves in Mice

Abstract

Morus nigra has been used to relieve pain in Brazilian folk medicine. This study was conducted to establish the antinociceptive properties of dichloromethane extract from leaves of M. nigra. The formalin, hot plate, and tail immersion tests as well as acetic acid-induced writhing were used to investigate the antinociceptive activity in mice. The extract at test doses of 100 and 300 mg/kg, p.o. clearly demonstrated antinociceptive activity in all tests. The extract administered at 300 mg/kg, p.o. had a stronger antinociceptive effect than indomethacin (5 mg/kg, p.o.) and morphine (10 mg/kg, p.o.), which supports previous claims for its traditional use.

Pharmacological Properties of Morus nigra L. (Black Mulberry) as A Promising Nutraceutical Resource

“…Because morusin is also purified from other mulberry plants, such as M. alba [11], M. australis [12] and M. lhou [13], this study result alone is insufficient to fully reflect the analgesic activity of M. nigra . Nine years later, Padilha et al [14] investigated the antinociceptive effect of methylene chloride extract of M. nigra leaves in mice. Similar to the results of de Souza et al [10], M. nigra leaves extract showed significantly and dose-dependently reduced acetic acid-induced writhing and formalin-induced pain and increased response latency period in a tail-immersion test and hot plate test without any acute toxicity when the dose of the extract was up to 300 mg/kg.…”

Bioactive Benzofuran Derivatives from Cortex Mori Radicis, and Their Neuroprotective and Analgesic Activities Mediated by mGluR1

“…Recently, the structures and bioactivities of polyphenols from Morus plants [ 14 ] and bioactive benzofurans [ 15 ] were reviewed. It should be noted that the extracts of some twigs [ 16 ], leaves [ 17 ], or root barks [ 18 ] of Morus species were used to relieve pain in folk medicine. To the best of our knowledge, only two compounds from genus Morus , cis -mulberroside A [ 19 ] and morusin [ 20 ], have been reported with analgesic activity to date, which is related to the traditional antirheumatic usage of Cortex Mori Radicis.…”

Anti-Inflammatory Effects of Campomanesia xanthocarpa Seed Extract Obtained from Supercritical CO2

“…e second phase of the test is associated with the action of inflammatory mediators (prostaglandin, histamine, and bradykinin) [38]. In that phase, the extract-treated group (p < 0.01) as well as the group that received diclofenac potassium (p < 0.05) presented a significant reduction in the time of nociceptive behavior in comparison with the vehicletreated animals (Figure 1(b)).…”