Antinociceptive effects of intrathecally administered F8Famide and FMRFamide in the rat

Gouardères, Christine; Sutak, Maaja; Zajac, Jean-Marie; Jhamandas, Khem

doi:10.1016/0014-2999(93)90095-y

Cited by 138 publications

(73 citation statements)

References 35 publications

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“…This confirms our previous observations showing that NPFF exerts an antinociceptive effect at the spinal level (Gouarderes et al, 1993c). Like NPFF, the spinal antinociceptive action of IDMe and 3D was dose-related in the tail-flick but not the in paw-pressure test.…”

Section: Discussionsupporting

confidence: 92%

“…Interestingly, on a molar basis, IDMe appears to be 10 to 20 times more potent than both NPFF and 3D in the thermal antinociception test. Like NPFF (Gouarderes et al, 1993c), its analogues produced sustained antinociception. This prolonged effect is unlikely to result from the action of intact peptide since NPFF analogues are degraded by mouse brain homogenates within 20 min in the absence of peptidase inhibitors (Gicquel et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Like morphine, NPFF decreased colonic bead expulsion in mice (Raffa & Jacoby, 1989;. In rats, intrathecal NPFF injection elicited a long-lasting spinal antinociception that was partly attenuated by naloxone (Gouarderes et al, 1993c).…”

Section: Introductionmentioning

confidence: 92%

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Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Gouardères

Jhamandas

Sutak

et al. 1996

British J Pharmacology

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1 Neuropeptide FF (NPFF) has been shown to produce antinociceptive effects and enhance morphineinduced antinociception after intrathecal (i.t.) injection. In this study, the spinal effects of two NPFF analogues, [D-Tyr',(NMe) (11 nmol 5 The results of this study show that spinal ,-and 6-opioid receptors play a role in antinociception produced by NPFF analogues. These results also suggest a role for NPFF in modulation of nociceptive signals at the spinal level.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Gouardères

Jhamandas

Sutak

et al. 1996

British J Pharmacology

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“…There is a large body of evidence that NPFF exhibits antiopioid properties; in rodents, morphine-induced analgesia decreased following administration of NPFF or NPFF analogues and increased, as stress-induced analgesia, in response to anti-NPFF antibody administration [6][7][8]. In contrast, intrathecal injections of NPFF analogues induced a long-lasting analgesia [9,10] by increasing opioid peptide release in the spinal cord through the functional blockade of presynaptic delta-opioid autoreceptors [11,12]. Recent data provided evidence that opioid and NPFF endogenous systems exert a tonic activity, NPFF counteracting tonic opioid analgesia under resting conditions [13].…”

mentioning

confidence: 99%

“…In contrast, intrathecal injections of NPFF analogues induced a long-lasting analgesia [9,10] by increasing opioid peptide release in the spinal cord through the functional blockade of presynaptic delta-opioid autoreceptors [11,12]. Recent data provided evidence that opioid and NPFF endogenous systems exert a tonic activity, NPFF counteracting tonic opioid analgesia under resting conditions [13].…”

mentioning

confidence: 99%

Identification of proNeuropeptide FF_A peptides processed in neuronal and non‐neuronal cells and in nervous tissue

Bonnard

Burlet‐Schiltz

Monsarrat

et al. 2003

European Journal of Biochemistry

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Peptides which should be generated from the neuropeptide FF (NPFF) precursor were identified in a neuronal (human neuroblastoma SH-SY5Y) cell line and in COS-7 cells after transient transfection of the human proNPFF A cDNA and were compared with those detected in the mouse spinal cord. After reverse-phase high performance liquid chromatography of soluble material, NPFF-related peptides were immunodetected with antisera raised against NPFF and identified by using on-line capillary liquid chromatography/ nanospray ion trap tandem mass spectrometry. Neuronal and non-neuronal cells generated different peptides from the same precursor. In addition to NPFF, SQA-NPFF (Ser-Gln-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) and NPAF were identified in the human neuroblastoma while only NPFF was clearly identified in COS-7 cells. In mouse, in addition to previously detected NPFF and NPSF, SPA-NPFF (Ser-Pro-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Pheamide), the homologous peptide of SQA-NPFF, were characterized. These data on intracellular processing of proNeuropeptide FFA are discussed in regard to the known enzymatic processing mechanisms. There is a large body of evidence that NPFF exhibits antiopioid properties; in rodents, morphine-induced analgesia decreased following administration of NPFF or NPFF analogues and increased, as stress-induced analgesia, in response to anti-NPFF antibody administration [6][7][8]. In contrast, intrathecal injections of NPFF analogues induced a long-lasting analgesia [9,10] by increasing opioid peptide release in the spinal cord through the functional blockade of presynaptic delta-opioid autoreceptors [11,12]. Recent data provided evidence that opioid and NPFF endogenous systems exert a tonic activity, NPFF counteracting tonic opioid analgesia under resting conditions [13]. NPFF is also implicated in morphine tolerance, morphine abstinence and also in several physiological processes, such as body thermoregulation, food intake and blood pressure regulation [7,[14][15][16][17][18][19][20][21].These pharmacological effects are mediated by two G-protein-coupled receptors, NPFF 1 and NPFF 2 , cloned in human and rat [22][23][24][25]. Pharmacological characterization of these receptors in recombinant cell lines showed a better selectivity of peptides deduced from proNPFF A sequence for NPFF 2 receptors binding, whereas proN-PFF B -derived peptides displayed a greater affinity for NPFF 1 receptors [26]. Autoradiographic studies performed on rat CNS with highly selective radioligands revealed the localization of both receptors in central nervous areas implicated in pain transmission [27]. The existence of two peptidergic systems of neurotransmission, mediated through NPFF 1 and NPFF 2 receptor stimulation by peptides generated by proNPFF B and proNPFF A processing, respectively, could explain the complex pharmacological effects of NPFF.The characterization of NPFF-related peptides generated by NPFF precursors processing is essential to identify peptides candidate to the role of neurotransmitter. This study f...

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Activation of neuropeptide FF neurons in the brainstem nucleus tractus solitarius following cardiovascular challenge and opiate withdrawal

et al. 1998

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Neuropeptide FF (NPFF), a morphine modulatory peptide, is localized within discrete autonomic regions including the brainstem nucleus tractus solitarius (NTS) and the parabrachial nucleus (PBN). We investigated the activation of NPFF neurons in the NTS of rats induced by cardiovascular challenge and centrally generated opiate withdrawal. For hypotensive stimulation, we used systemic infusions of sodium nitroprusside (NP) or hemorrhage (HEM), and hypertension was achieved by intravenous phenylephrine (PHENYL) or angiotensin II (AII). In rats that received continuous intracerebroventricular injections of morphine, intraperitoneal injections of naloxone precipitated behavioural signs of opioid withdrawal. Activated NTS neurons were identified by using a combined immunohistochemistry for Fos and NPFF, and neurons projecting to the PBN were determined with a retrograde tracer. HEM, administration of vasoactive drugs, and opiate withdrawal produced a very robust activation of NTS neurons. In NP and HEM groups, 25.6+/-3.2% and 7.6+/-1.3% of NPFF neurons were activated, respectively. Lesser numbers of NPFF neurons were activated in the PHENYL (4.6+/-1.6%) and AII (2.4+/-0.8%) groups. However, following opiate withdrawal, virtually no Fos expression was observed in NPFF neurons. NPFF neurons activated during NP infusion constituted the largest number of cells projecting to the PBN. This study shows that NPFF neurons in NTS that project to the PBN respond selectively to NP as opposed to other cardiovascular challenges or opiate withdrawal. These data support an emerging and important role for NPFF in the context of central cardiovascular regulation.

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Antinociceptive effects of intrathecally administered F8Famide and FMRFamide in the rat

Cited by 138 publications

References 35 publications

Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Identification of proNeuropeptide FF_A peptides processed in neuronal and non‐neuronal cells and in nervous tissue

Activation of neuropeptide FF neurons in the brainstem nucleus tractus solitarius following cardiovascular challenge and opiate withdrawal

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Antinociceptive effects of intrathecally administered F8Famide and FMRFamide in the rat

Cited by 138 publications

References 35 publications

Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Role of opioid receptors in the spinal antinociceptive effects of neuropeptide FF analogues

Identification of proNeuropeptide FFA peptides processed in neuronal and non‐neuronal cells and in nervous tissue

Activation of neuropeptide FF neurons in the brainstem nucleus tractus solitarius following cardiovascular challenge and opiate withdrawal

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Identification of proNeuropeptide FF_A peptides processed in neuronal and non‐neuronal cells and in nervous tissue