Antinuclear Antibodies in Dogs with Leishmaniasis

Lucena, R.; Ginel, Pedro J.; López, R.; Novales, M.; Martín, E; Molleda, J. M.

doi:10.1111/j.1439-0442.1996.tb00451.x

Cited by 10 publications

(7 citation statements)

References 11 publications

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“…A study from Spain reported ANAs in 15.9% of dogs with disease manifestations that were attributed to leishmaniasis. 4 That study found no cause-and-effect relationship between the presence of ANAs and renal failure in dogs with leishmaniasis. Two subsequent studies from Spain and Italy found the incidence of ANAs in dogs naturally infected with L infantum to be 10 and 47%, respectively.…”

Section: Discussionmentioning

confidence: 84%

“…Numerous reports have identified ANAs in dogs with chronic bacterial and protozoal infections, particularly leishmaniasis. [3][4][5][6][7][8][9] Some evidence also exists to support the theory that transmissible agents may induce ANAs in dogs as well as in people. In one study, an increased occurrence of ANAs was detected in sera from clinically normal dogs owned by humans concurrently diagnosed with SLE.…”

mentioning

confidence: 99%

“…In endemic regions, ANAs have been detected at varying frequencies in dogs that are reactive to L infantum antigens. [4][5][6] Therefore, evaluation of L infantum-infected Foxhounds for the presence of ANAs afforded the opportunity to study this phenomenon in dogs from a nonendemic region.…”

mentioning

confidence: 99%

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Antinuclear Antibodies Can Be Detected in Dog Sera Reactive to Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis, or Leishmania infantum Antigens

Smith¹,

Tompkins²,

Breitschwerdt³

2004

J Vet Int Med

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The presence of antinuclear antibodies (ANAs) is used to support a clinical diagnosis of systemic lupus erythematosus (SLE) in dogs. However, clinicians must interpret the detection of ANAs with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ANA titers. Retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and Bartonella vinsonii (berkhoffii), Ehrlichia canis, or Rickettsia rickettsii antigens between 1990 and 2000. When analyzed on the basis of reactivity to a specific infectious agent, 75% of the B vinsonii (berkhoffii) seroreactors, 16.7% of the E canis seroreactors, and 0% of the R rickettsii seroreactors had concurrent ANAs. Subsequent prospective testing did not detect ANAs in convalescent sera from dogs experimentally infected with B vinsonii (berkhoffii), E canis, or R rickettsii. However, 10-20% B vinsonii (berkhoffii), E canis, or Leishmania infantum reactive sera from naturally infected dogs contained ANAs. In addition, 45% of sera from dogs that are reactive to multiple vectorborne organisms were more likely to contain ANAs when compared to sera from dogs reactive to only 1 test antigen. When interpreting the relevance of seroreactivity to nuclear antigens, clinicians should recognize that dogs with seroreactivity to B vinsonii (berkhoffii), E canis, or L infantum antigens (especially those with seroreactivity to more than one of these pathogens) may produce ANAs.

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

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Antinuclear Antibodies Can Be Detected in Dog Sera Reactive to Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis, or Leishmania infantum Antigens

Smith¹,

Tompkins²,

Breitschwerdt³

2004

J Vet Int Med

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“…Secondary renal disease. Underlying disorders causing secondary renal disease and subsequent increases in P‐creatinine include babesiosis, 166,188,189 leishmaniasis, 190–194 leptospirosis, 195–198 borreliosis, 199–200 trypanosomiasis, 201,202 heartworm disease, 203 encephalitozoonosis, 204 malignant histiocytosis, 205 pyometra, 206–209 experimental intestinal obstruction, 210 gastric dilatation/torsion, 211 diabetes mellitus, 212,213 and hypercalcemia caused by hyperparathyroidism 214 or lymphoma 215 . The magnitude of increase in P‐creatinine differed greatly according to the severity of the underlying disorder.…”

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confidence: 99%

Creatinine in the Dog: A Review

Braun

Lefebvre

Watson

2003

Veterinary Clinical Pathol

189

154

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Creatinine is the analyte most frequently measured in human and veterinary clinical chemistry laboratories as an indirect measure of glomerular filtration rate (GFR). Although creatinine metabolism and the difficulties of creatinine measurement have been reviewed in human medicine, similar reviews are lacking in veterinary medicine. The aim of this review is to summarize information and data about creatinine metabolism, measurement, and diagnostic significance in the dog. Plasma creatinine originates from the degradation of creatine and creatine phosphate, which are present mainly in muscle and in food. Creatinine is cleared by glomerular filtration with negligible renal secretion and extrarenal metabolism, and its clearance is a good estimate of GFR. Plasma and urine creatinine measurements are based on the nonspecific Jaffé reaction or specific enzymatic reactions; lack of assay accuracy precludes proper interlaboratory comparison of results. Preanalytical factors such as age and breed can have an impact on plasma creatinine (P-creatinine) concentration, while many intraindividual factors of variation have little effect. Dehydration and drugs mainly affect P-creatinine concentration in dogs by decreasing GFR. P-creatinine is increased in renal failure, whatever its cause, and correlates with a decrease in GFR according to a curvilinear relationship, such that P-creatinine is insensitive for detecting moderate decreases of GFR or for monitoring progression of GFR in dogs with severely reduced kidney function. Low sensitivity can be obviated by determining endogenous or exogenous clearance rates of creatinine. A technique for determining plasma clearance following IV bolus injection of exogenous creatinine and subsequent serial measurement of P-creatinine does not require urine collection and with additional studies may become an established technique for creatinine clearance in dogs.

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“…The production of circulating immune complexes (Lopez et al, 1996) and autoantibodies such as antinuclear antibodies (anti-DNA, RNA, nuclear proteins and their molecular complexes), and anti-mammalian basal membrane glycoproteins and cerebrosides, and anti-Histone, was previously described in L. infantum-infected dog's sera (Pateraki et al, 1983;Lucena and Ginel, 1998;Bell et al, 1997;Smith et al, 2004). Ferrer (1992) and Lucena et al (1996) reported a 30 and 15.9% incidence of antinuclear antibodies in dogs with natural Leishmania infection, respectively. Pateraki et al (1983) demonstrated by ELISA that 250 (95%) and 248 (94%) dogs out of 260 with cVL, all positive for L. donovani antigens, have anti-actin and anti-tubulin IgG autoantibodies, respectively.…”

Section: A C C E P T E Dmentioning

confidence: 95%

Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs

Chaabouni

Elandoulsi

Mhadhbi

et al. 2018

Veterinary Parasitology

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Leishmania (L.) infantum-infected dogs may present with a large range of clinical signs, from apparently healthy with no or few (asymptomatic dogs, AD) to several clinical signs indicators of active infection (symptomatic dogs, SD). The present study is justified by the conflicting reports describing that either L. infantum-specific IgG1 or IgG2 antibodies may be used as isotype marker of the asymptomatic infection status and by the lack, to our knowledge, of previous analysis of the IgG sub-classes autoantibody repertoires of Leishmania-infected dogs. On the basis of clinical evaluation and laboratory testing (IFAT, parasitological examination of Giemsa-stained lymph node smears, L. infantum antigens-ELISA of total (Tot) IgG), 131 dogs were categorized as SD, asymptomatic seronegative (AND) or seropositive dogs (APD) from surrounding areas, and as negative control dogs (CTD). ELISA based on leishmanial native antigens or recombinant LACK and LeIF proteins showed that SD produce higher levels of specific Tot IgG, IgG1 and IgG2 antibodies than APD, and that for both clinical stages, the antibody titers of IgG2 isotype were constantly higher than those of the IgG1. The seroprevalences of Tot IgG, IgG2 did not differ between APD and SD groups (97 and 97% in SD; 100 and 96% in APD, respectively) whereas that of IgG1 was slightly lower in SD (88% of APD versus 82% of SD). The autoantibody repertoires were analyzed by ELISA using HEp-2 extracts, ds-DNA, human albumin and transferrin as self-antigens and by Western blot using HEp-2 proteins. ELISA results' indicated that APD develop higher levels of IgG1 autoantibodies, and higher seroprevalence (50% and 26% in APD and SD, respectively), contrasting with lower levels and seroprevalences of Tot IgG and IgG2 (43 and 68% for APD; 100 and 74% for SD). Interestingly, SD showed a stronger IgG1 and particularly IgG2 reactivity with transferrin, an iron-binding protein, than APD and AND. Western blotting experiments produced heterogeneous IgG1 and IgG2 inter- and intra-groups reactivity profiles towards HEp-2 proteins, to identify a specific antigenic profile. Generated data from competitive HEp-2-ELISA using leishmanial antigens as inhibitors were in favor that IgG1 antibodies are predominantly autoantibodies to self-antigens in APD whereas they are mainly cross-reactive (Leishmania/self-antigens) in SD.

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Antinuclear Antibodies in Dogs with Leishmaniasis

Cited by 10 publications

References 11 publications

Antinuclear Antibodies Can Be Detected in Dog Sera Reactive to Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis, or Leishmania infantum Antigens

Antinuclear Antibodies Can Be Detected in Dog Sera Reactive to Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis, or Leishmania infantum Antigens

Creatinine in the Dog: A Review

Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs

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