Antinuclear Antibody Induced by Procainamide

Blomgren, Stephen E.; Condemi, John J.; Bignall, Margaret C.; Vaughan, John H.

doi:10.1056/nejm196907102810203

Cited by 112 publications

(24 citation statements)

References 7 publications

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“…Prospective studies have revealed that PA may induce antinuclear antibodies in 50-83% of individuals, and that 12-29% of PA-treated patients develop a syndrome resembling systemic lupus erythematosus (SLE) (7)(8)(9)(10). The development of autoimmunity induced by PA is influenced by factors that affect the serum level of this agent.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro.

Ochi¹,

Goldings²,

Lipsky³

et al. 1983

J. Clin. Invest.

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A B S T R A C T Procainamide (PA) induces the production of a number of autoantibodies in a high proportion of treated individuals and in some a syndrome closely resembling systemic lupus erythematosus. The mechanism underlying this action of PA is unclear. To examine the possibility that PA might induce autoantibody formation by altering normal immunoregulatory mechanisms, the action of this drug on an in vitro model of antibody formation in man was examined. PA was found to augment the generation of immunoglobulin-secreting cells (ISC) from human peripheral blood mononuclear cells (PBM) in response to pokeweed mitogen but had no effect on pokeweed mitogen-induced tritiated thymidine incorporation. When purified populations of B and T cells were used, PA enhanced the generation of ISC in B-cell cultures supported by untreated T cells but not by T cells treated with mitomycin C. These results indicate that PA augmented B-cell responses by inhibiting suppressor T-cell activity and not by augmenting helper T-cell or B-cell function. N-Acetyl-procainamide had no effect on the generation of ISC in this system.The effect of PA on concanavalin A (Con A)-induced suppressor cell activity was also examined to determine whether PA altered the generation or expression of suppressor T-cell function. PBM were cultured with 30 Aig/ ml of Con A for 48 h to generate suppressor cells. When these were co-cultured with fresh PBM, the number of ISC generated was decreased by 58.1±3.4% (mean ±SEM, n = 6). Cells that had been similarly incubated without Con A were not inhibitory. The addition of PA to the Con A-stimulated cultures inhibited the generation of suppressor cells as indicated by the fact that the response of fresh cells co-cultured with the Con A-stimulated cells was diminished by only 27.2±4.3%. In this system too, N-acetyl-procaimamide had no effect. By contrast, adding PA only to the co-culture of Con A-

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Section: Introductionmentioning

confidence: 99%

Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro.

Ochi¹,

Goldings²,

Lipsky³

et al. 1983

J. Clin. Invest.

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“…An SLE-like syndrome has also been reported following administration of various drugs, e.g. hydrallazine (Perry & Schroeder, 1954), procainamide (Blomgren, Condemi, Bignall & Vaughan, 1969), and some anticonvulsant medications (Benton, Tynes, Register, Alford & Holley, 1962), but in this case the presence of antinuclear antibodies is associated with absence of antibodies directed against DNA (Harpey, 1973). In this investigation the incidence of SLE associated antibodies was assessed in a group of 100 unselected patients with acute psychiatric disease and compared with that in group of 112 osteoarthritic controls matched for age and sex.…”

mentioning

confidence: 99%

The specificity of the action of hyoscine on human learning [proceedings].

Crow¹,

Grove-White²,

Dg³

1975

Brit J Clinical Pharma

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“…Procainamide-treated patients are of special interest because they frequently develop a syndrome similar to idiopathic SLE (22)(23)(24)(25)(26). Anti-dDNA is often found (3,22), but its relationship to the clinical manifestations have not been established.…”

mentioning

confidence: 99%

Anti‐DNA antibody in procainmide‐induced lupus erythematosus. Determination using DNA fractionated by methylated albumin‐kieselguhr chromatography

Winfield

Davis

1974

Arthritis & Rheumatism

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Both putatively native(n) commercial calf thymus DNA and I4C labeled human KB DNA contained significant amounts of denatured DNA (dDNA) as determined by methylated albumin-kieselguhr chromatography. The proportion of dDNA in these preparations was sufficient to cause confusion regarding the antigenic specificity of anti-DNA antibody when unfractionated DNA was used in either the counterimmunoelectrophoresis or the Farr assays for anti-DNA antibody. Serum from patients with systemic lupus erythematosus contained both anti-nDNA and anti-dDNA antibodies. About 50 % of patients with symptomatic procainamide-induced lupus (PLE) exhibited anti-dDNA; none had anti-nDNA. Procainamide treated patients with a positive ANF who did not have symptomatic PLE had neither anti-dDNA nor anti-nDNA. Considerable data sumort the concept & Ithat native DNA (nDNA) and anti-nDNA antibody are of primary pathogenetic im-

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Antinuclear Antibody Induced by Procainamide

Cited by 112 publications

References 7 publications

Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro.

Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro.

The specificity of the action of hyoscine on human learning [proceedings].

Anti‐DNA antibody in procainmide‐induced lupus erythematosus. Determination using DNA fractionated by methylated albumin‐kieselguhr chromatography

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