Antinutritional effects of the chitin-binding lectin from Microgramma vacciniifolia rhizome (MvRL) on Sitophilus zeamais

Albuquerque, Lidiane Pereira de; Procópio, Thamara Figueiredo; Guedes, Caio César da Silva; Pontual, Emmanuel Viana; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique

doi:10.1016/j.jspr.2020.101652

Cited by 10 publications

(6 citation statements)

References 46 publications

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“…The insect midgut is a main target of the insecticidal mechanisms of lectins, which can involve the resistance to digestion by enzymes from the insect gut, the ability to interact with N ‐acetylglucosamine residues at the peritrophic membrane, the induction of deformation and death of surface cells in the absorptive gut epithelium, as well as imbalance of digestive enzyme activities (Lima et al, 2017, 2018; Napoleão et al, 2019; Van Damme, 2021). These morphophysiological alterations at midgut level may lead to antinutritional effects, impairment of reproductive fitness, and often to death (Albuquerque et al, 2020; Napoleão et al, 2019). Lectins have shown larvicidal effect against Ae.…”

Section: Introductionmentioning

confidence: 99%

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Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Nova

Almeida

Procópio

et al. 2022

Arch Insect Biochem Physiol

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This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3) larvae to the saline extract of Opuntia ficus‐indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at ¼ LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50; the development and reproduction of the emerged adults were evaluated after a recovery period of 9 days. The resistance of proteins in the extract to hydrolysis by L3 digestive enzymes and histomorphological alterations in the larval midgut were also investigated. The extract contained lectin, flavonoids, cinnamic derivatives, terpenes, steroids, and reducing sugars. It showed a LC50 of 3.71% for 48 h. The data indicated mean survival times similar in control and extract treatments. It was observed development delay in extract‐treated groups, with a lower number of adults than in control. However, the females that emerged laid similar number of eggs in control and treatments. Histological evaluation revealed absence of bacterial and fungal microorganisms in the food content in midguts from larvae treated with cladode extract. Electrophoresis revealed that three polypeptides in the extract resisted to hydrolysis by L3 digestive proteases for 90 min. The lectin activity was not altered even after 24‐h incubation with the enzymes. In conclusion, the extract from O. ficus‐indica can delay the development of Ae. aegypti larvae, which may be linked to induction of an axenic environment at larval midgut and permanence of lectin activity even after proteolysis.

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Section: Introductionmentioning

confidence: 99%

“…These morphophysiological alterations at midgut level may lead to antinutritional effects, impairment of reproductive fitness, and often to death (Albuquerque et al, 2020;Napoleão et al, 2019). Lectins have shown larvicidal effect against Ae.…”

Section: Introductionmentioning

confidence: 99%

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Nova

Almeida

Procópio

et al. 2022

Arch Insect Biochem Physiol

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“…For example, Macedo et al [ 27 ] reported that Bauhinia monandra Kurz (Fabales: Fabaceae) leaf lectin caused significant mortality in Callosobruchus maculatus and Zabrotes subfasciatus . It seems that the deleterious effects of plant lectins on insects are due to the toxicity and antinutritional effects of lectins [ 1 , 27 , 44 , 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Napoleao et al [ 1 ] reported that a lectin from Myracrodruon urundeuva leaf had inhibitory effects on digestive enzymes α-amylase and proteases in adults of Sitophilus zeamais . Albuquerque et al [ 47 ] showed that lectin extracted from Microgramma vacciniifolia rhizome reduced α-amylase and β-glucosidase activities in S . zeamais .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Potential of a Lectin Extracted from Polygonumpersicaria L. as a Biorational Agent against Sitophilusoryzae L.

et al. 2022

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Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), is one of the most destructive stored-product pests that is resistant to a wide range of chemical insecticides. In the present study, we investigated whether a lectin extracted from Polygonum persicaria L. (PPA) can be used as a biorational agent to control such insect pests. Along with the lethal digestive assay, the sub-lethal insecticidal activities of PPA, including the effects on digestive, detoxifying, and antioxidant enzyme activities, were evaluated against S. oryzae adults. The effect of feeding a diet containing PPA and carob extract as a food attractant on the mortality of S. oryzae adults was also investigated. Feeding on the diet containing PPA resulted in a significant mortality of S. oryzae adults with a LC50 (Lethal Concentration to kill 50% of insects) of 3.68% (w/w). The activity of digestive enzymes, including α-amylase, α-glucosidase, TAG-lipase, trypsin, chymotrypsin, elastase, and carboxy- and aminopeptidase, were decreased by the sub-lethal concentration of PPA. Detoxifying and antioxidant enzymes, including esterase, superoxide dismutase, catalase, glutathione-S-transferase, ascorbate peroxidase, glucose 6-phosphate dehydrogenase, and malondialdehyde, were activated in adults affected by PPA. These findings indicated that PPA, in addition to causing digestive disorders, leads to oxidative stress in S. oryzae. The presence of carob extract had no effect on the PPA-induced mortality of the insect. According to the results of the present study, PPA has promising insecticidal efficiency against S. oryzae. In addition, the usage of PPA with a food attractant carob extract in bait traps can be recommended as a new biorational formulation in S. oryzae management.

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“…Plant lectins have been found to be biologically active against a variety of insects. Chitin-binding lectins derived from Microgramma vacciniifolia rhizome lectin (MvRL) showed anti-nutritional effects on survival, feeding, and nutrition of Sitophilus zeamais adults ( de Albuquerque et al, 2020 ). Moringa oleifera seeds containing a water-soluble lectin (WSMoL) negatively impacted the physiology of the pest Sitophilus zeamais , which could have long-term consequences ( de Oliveira et al, 2020 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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Antinutritional effects of the chitin-binding lectin from Microgramma vacciniifolia rhizome (MvRL) on Sitophilus zeamais

Cited by 10 publications

References 46 publications

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Evaluation of the Potential of a Lectin Extracted from Polygonumpersicaria L. as a Biorational Agent against Sitophilusoryzae L.

Biotechnological Approaches for Host Plant Resistance to Insect Pests

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