Antiobesity Activity of Two Polyherbal Formulations in High-Fat Diet-Induced Obese C57BL/6J Mice

Abstract

Obesity and overweight have posed a severe threat to humanity, needing urgent efforts for the development of safe and effective therapeutic interventions. In this research work, we have developed two polyherbal formulations A and B basically consisting of Helianthus tuberosus root powder (also called inulin of synanthrin) along with other herbs for the treatment of obesity. Evaluation of the antioxidant activity of both formulations using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 ′ -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays showed good antioxidant potentials. Both formulations A and B showed good antiobesity activity on a diet-induced obesity (DIO) model of mice by effectively lowering the body weight of mice compared to the high-fat diet (HFD) control mice, mainly by reducing the food efficiency ratio (FER). Furthermore, both formulations ameliorated lipoprotein misbalances induced by obesity and thus decreased the atherogenic index. Treatment with both formulations significantly decreased the liver and epididymal white adipose tissue (WAT) weight. This was supported by the improvement in steatosis of the liver and reduced hypertrophy in WAT on histological examination. In addition, formulations A and B have been seen as effective in controlling fasting blood glucose levels probably by alleviating HFD-induced insulin resistance. All of these results collectively suggest that formulations A and B serve as potentially safe and effective herbal interventions to control obesity and its comorbidities.

Evaluation of Anti-Obesity and Antidiabetic Activities of Orostachys japonicus in Cell and Animal Models

“…Then, the mice were randomly divided into four groups (6 mice in each group), as shown in Table 4. The mice were provided with their respective diets (normal and high-fat diets) and water ad libitum throughout the experiment (14 weeks) [55]. Samples of butanol fraction (suspension prepared in PBS) were administered orally.…”

Anti-obesity effect of the bacterial product nisin in an NIH Swiss mouse model

“…However, there are some side effects associated with these treatments. Other herbal medications have also been introduced, but there are information gaps that should be filled with further studies [4][5][6][7].…”

Yellow Himalayan Raspberry (Rubus ellipticus Sm.): Ethnomedicinal, Nutraceutical, and Pharmacological Aspects

“…This approach, on one hand, would help validate the use of these plants as traditional medicine and nutraceuticals, and on the other hand, increases the likelihood of the discovery of lead compounds compared to random screening [ 8 , 9 ]. Furthermore, polyherbal formulation synergistic therapeutic effects and reduced adverse effects due to individual ingredients can be developed with low doses of multiple ingredients for the management of diseases [ 10 , 11 , 12 ]. Many bioactive compounds with beneficial effects for various chronic diseases such as diabetes [ 13 ], hypercholesterolemia [ 14 , 15 ], high blood pressure [ 16 ], cirrhosis [ 17 , 18 ], chronic obstructive pulmonary disease (COPD) [ 19 , 20 ], cancer [ 21 ], bacterial infections, aging-related degenerative diseases, and autoimmune diseases [ 22 ] have been identified.…”