Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats

Abstract

BackgroundRecent studies have reported the preventive effects of probiotics on obesity. Among commensal bacteria, bifidobacteria is one of the most numerous probiotics in the mammalian gut and are a type of lactic acid bacteria. The aim of this study was to assess the antiobesity and lipid-lowering effects of Bifidobacterium spp. isolated from healthy Korean on high fat diet-induced obese rats.MethodsThirty-six male Sprague-Dawley rats were divided into three groups as follows: (1) SD group, fed standard diet; (2) HFD group, fed high fat diet; and (3) HFD-LAB group, fed high fat diet supplemented with LAB supplement (B. pseudocatenulatum SPM 1204, B. longum SPM 1205, and B. longum SPM 1207; 108 ~ 109 CFU). After 7 weeks, the body, organ, and fat weights, food intake, blood serum levels, fecal LAB counts, and harmful enzyme activities were measured.ResultsAdministration of LAB reduced body and fat weights, blood serum levels (TC, HDL-C, LDL-C, triglyceride, glucose, leptin, AST, ALT, and lipase levels), and harmful enzyme activities (β-glucosidase, β-glucuronidase, and tryptophanase), and significantly increased fecal LAB counts.ConclusionThese data suggest that Bifidobacterium spp. used in this study may have beneficial antiobesity effects.

The Effect of Synbiotic Supplementation on Body Composition and Lipid Profile in Patients with NAFLD: A Randomized, Double Blind, Placebo-Controlled Clinical Trial Study

“…Change in cholesterol level was not unexpected as over several years, more and more experimental and clinical trials indicated that probiotics could lead to a decrease in serum cholesterol (22)(23)(24)(25)(26). Findings of a recent metaanalysis indicated that probiotics had a significantly good effect on normalizing total cholesterol, as we observed (27).…”

“…Moreover, fermentation products of lactic acid bacteria inhibit cholesterolsynthesizing enzymes and thus reduce cholesterol production. The bacteria may facilitate elimination of cholesterol in feces and may inhibit its reabsorption into the body; in addition, these bacteria may interfere with the recycling of bile salt (a metabolic product of cholesterol) (22,24). We observed no effect on HDL level, may be HDL requires longterm intervention to change or there are unknown mechanisms in this regard (27).…”

Use of pigs as a potential model for research into dietary modulation of the human gut microbiota

“…Prebiotic components with a positive impact on obesity include oligofructose, which stimulated caecal bifidobacteria numbers in mice fed a high-fat diet and reduced the metabolic disease (202) . In a study with rats, An et al (203) found beneficial anti-obesity effects due to supplementation with the probiotic Bifidobacterium spp. to a highfat diet (Bifidobacterium pseudocatenulatum SPM 1204, Bifidobacterium longum SPM 1205, Bifidobacterium longum 1207), with reduced body and fat weights and blood serum levels (for example, TAG, glucose, leptin) and increased total faecal lactic acid bacteria counts in the probiotic-supplemented high-fat diet compared with the diet devoid of probiotics.…”

Consumption of Vitamin B6 Reduces Fecal Ratio of Lithocholic Acid to Deoxycholic Acid, a Risk Factor for Colon Cancer, in Rats Fed a High-Fat Diet

“…Cecal organic acids were measured by the internal standard method using an HPLC equipped with an Aminex HPX-87H ion exclusion column (7.8 mm i.d.ϫ30 cm, Bio-Rad, Richmond, CA) (27,28). Activities of harmful fecal enzymes, including ␤-glucosidase, ␤-glucuronidase, tryptophanase, and urease, were determined according to the methods described elsewhere (29). Data were expressed as the meansϮSE.…”