Antiosteoporosis effect of geraniin on ovariectomy‐induced osteoporosis in experimental rats

Wang, Xiuhui; Wang, Minghui; Cui, Xu; Li, Zhuokai; Guo, Shengyang; Gao, Feng; Ma, Ming; Wang, Zhe

doi:10.1002/jbt.22774

Cited by 8 publications

(13 citation statements)

References 22 publications

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“…Ovariectomized rodents are very useful animal models to study geriatric diseases. For example, ovariectomy is a frequently used technique to induce osteoporosis in rodent models (17)(18)(19). Rodrigues et al (20) found that physical exercise could alter the morphology of the liver of ovariectomized Ldlr -/mice.…”

Section: Introductionmentioning

confidence: 99%

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Ovariectomy Impaired Hepatic Glucose and Lipid Homeostasis and Altered the Gut Microbiota in Mice With Different Diets

Lei

Yang

et al. 2021

Front. Endocrinol.

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The lower incidence of metabolic diseases of women than men and the increasing morbidity of metabolic disorders of menopausal women indicated that hormones produced by ovaries may affect homeostasis of glucose and lipid metabolism, but the underlying mechanisms remain unclear. To explore the functions of ovaries on regulating glucose and lipid metabolism in females, 8 weeks old C57BL/6 mice were preformed ovariectomy and administrated with normal food diet (NFD) or high fat diet (HFD). Six weeks after ovariectomy, blood biochemical indexes were tested and the morphology and histology of livers were checked. The expression levels of genes related to glucose and lipid metabolism in liver were detected through transcriptome analysis, qPCR and western blot assays. 16S rDNA sequence was conducted to analyze the gut microbiota of mice with ovariectomy and different diets. The serum total cholesterol (TC) was significantly increased in ovariectomized (OVX) mice fed with NFD (OVXN), and serum low density lipoprotein-cholesterol (LDL-C) was significantly increased in both OVXN mice and OVX mice fed with HFD (OVXH). The excessive glycogen storage was found in livers of 37.5% mice from OVXN group, and lipid accumulation was detected in livers of the other 62.5% OVXN mice. The OVXN group was further divided into OVXN-Gly and OVXN-TG subgroups depending on histological results of the liver. Lipid drops in livers of OVXH mice were more and larger than other groups. The expression level of genes related with lipogenesis was significantly increased and the expression level of genes related with β-oxidation was significantly downregulated in the liver of OVXN mice. Ovariectomy also caused the dysbiosis of intestinal flora of OVXN and OVXH mice. These results demonstrated that hormones generated by ovaries played important roles in regulating hepatic glucose and lipid metabolism and communicating with the gut microbiota in females.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ovariectomy Impaired Hepatic Glucose and Lipid Homeostasis and Altered the Gut Microbiota in Mice With Different Diets

Lei

Yang

et al. 2021

Front. Endocrinol.

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“…The inflammatory immune response occurs through activation of immune cells which secreted proinflammatory cytokines, such as TNF-α, IL-1α and IL-6. Experimental and clinical studies have been indicated that TNF-α, IL-1α and IL-6 are cytokines associated with infection, trauma, and sepsis syndrome (4,20). TNF-α starts and, regulates cytokinesis cascade during the inflammatory response.…”

Section: Resultsmentioning

confidence: 99%

“…Another study showed that cod bone gelatin treatment can positively affect bone improvement by suppressing the release of TNF-α, IL-1 and IL-6, which often increases post-OVX (37). Many studies revealed the anti-inflammatory effects of curcumin, vanillic acid, soybean, geraniin, vicenin in the OVX rats (5,16,20). In this study, we used BA to test the anti-inflammatory effect against the inflammatory changes associated with OVX.…”

Section: Resultsmentioning

confidence: 99%

Anti- inflammatory effect of boric acid on cytokines in ovariectomy-induced rats

Tekeli

Aşıcı

Bildik

2022

Cell Mol Biol (Noisy-le-grand)

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The increase in the rate of inflammation in the post-menopause period also leads to a significant increase in the use of anti-inflammatory agents. This study aimed to investigate the effect of BA supplementation on pro-and anti-inflammatory cytokines in ovariectomy (OVX) induced rats. A total of 48 nonpregnant female Wistar albino rats (80-100 g) were used in the experiment. Forty-eight rats were divided into six equal groups (n=8): Control, OVX, OVX+5 mg/kg BA (OVX+BA5), OVX+10 mg/kg BA (OVX+BA10), 5 mg/kg BA (BA5), 10 mg/kg BA (BA10). Serum TNF-α cytokine levels of rats in the OVX group were higher than in control rats (P<0.05). TNF-α levels were significantly reduced in the OVX-induced rats with 5 mg/kg BA and 10 mg/kg BA supplementation (P<0.05). While serum IL-1α and IL-6 levels were not different between OVX and control rats, serum IL-3 levels were low (P<0.05) and not affected by 5 mg/kg and 10 mg/kg BA supplementation. Serum IL-11 levels increased significantly in the OVX rats with 5 mg/kg and 10 mg/kg BA supplementation (P<0.05). As far as we know, certain doses (5 and 10 mg/kg) of BA are the first study on the prevention of increased inflammation in rats induced by OVX. Results suggest that the supplementation of BA regulates the inflammatory changes associated with OVX and thus has beneficial for menopause management.

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“…In previous studies, selected flavonoids were shown to induce osteoblastic differentiation and to inhibit osteoclastogenesis [ 14 , 15 , 16 , 17 ]. Flavonoids were also reported to induce several anti-OS activities, including cell cycle arrest, induction of apoptosis and inhibition of invasion and metastasis, suggesting a therapeutic potential [ 18 , 19 , 20 , 21 , 22 , 23 ]. Concerning current OS therapy, the anthracycline doxorubicin (Dox) is the most commonly prescribed agent to treat this cancer.…”

Section: Introductionmentioning

confidence: 99%

3′,4′-Dihydroxyflavonol Modulates the Cell Cycle in Cancer Cells: Implication as a Potential Combination Drug in Osteosarcoma

et al. 2021

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New agents are demanded to increase the therapeutic options for osteosarcoma (OS). Although OS is the most common bone cancer in children and adolescents, it is considered a rare disorder. Therefore, finding adjuvant drugs has potential to advance therapy for this disease. In this study, 3′,4′-dihydroxyflavonol (DiOHF) was investigated to assess the effects in OS cellular models in combination with doxorubicin (Dox). MG-63 and U2OS human OS cells were exposed to DiOHF and Dox and tested for cell viability and growth. To elucidate the inhibitory effects of DiOHF, additional studies were conducted to assess apoptosis and cell cycle distribution, gene expression quantification of cell cycle regulators, and cytokinesis-block cytome assay to determine nuclear division rate. DiOHF decreased OS cell growth and viability in a concentration-dependent manner. Its combination with Dox enabled Dox dose reduction in both cell lines, with synergistic interactions in U2OS cells. Although no significant apoptotic effects were detected at low concentrations, cytostatic effects were demonstrated in both cell lines. Incubation with DiOHF altered cell cycle dynamics and resulted in differential cyclin and cyclin-dependent kinase expression. Overall, this study presents an antiproliferative action of DiOHF in OS combination therapy via modulation of the cell cycle and nuclear division.

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Antiosteoporosis effect of geraniin on ovariectomy‐induced osteoporosis in experimental rats

Cited by 8 publications

References 22 publications

Ovariectomy Impaired Hepatic Glucose and Lipid Homeostasis and Altered the Gut Microbiota in Mice With Different Diets

Ovariectomy Impaired Hepatic Glucose and Lipid Homeostasis and Altered the Gut Microbiota in Mice With Different Diets

Anti- inflammatory effect of boric acid on cytokines in ovariectomy-induced rats

3′,4′-Dihydroxyflavonol Modulates the Cell Cycle in Cancer Cells: Implication as a Potential Combination Drug in Osteosarcoma

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